Journal
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
Volume 120, Issue -, Pages 192-202Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.ibiod.2017.02.020
Keywords
Monochlorobenzoate isomer; Degradation; Dechlorination; Dioxygenase
Funding
- National Key Research and Development Program of China [2016YFD0800203]
- Fundamental Research Funds for the Central Universities [KYZ201422]
- joint National Natural Science Foundation of China and the Israel Science Foundation Research Program [31461143009]
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Three monochlorobenzoate isomer-utilizing bacterial strains, Pseudomonas sp. 2-CBA, Pseudomonas sp. 3-CBA, and Hydrogenophaga sp. 4-CBA, were isolated from a haloaromatic-contaminated soil in a disused chemical factory in Nanjing, China. Compared to strain 3-CBA and strain 4-CBA, which could only utilize its corresponding monochlorobenzoate isomer (3-chlorobenzoate and 4-chlorobenzoate) as the sole carbon source for growth, strain 2-CBA could utilize both 2-chlorobenzoate and 3-chlorobenzoate for growth. The three monochlorobenzoate isomers were catabolized via three different dechlorination pathways in the three isolates, respectively. 3-Chlorobenzoate was initially catabolized by the benzoate 1,2-dioxygenase (BenABCD) in strain 3-CBA, whereas 2-chlorobenzoate was catabolized by the hybrid 2-halobenzoate 1,2-dioxygenase system in strain 2-CBA, which comprised of the terminal oxygenase component of 2-halobenzoate dioxygenase system (CbdAB) and the reductase component of benzoate 1,2-dioxygenase system (BenC). For 4-chlorobenzoate, it was catabolized via the classic hydrolytic dehalogenation pathway in strain 4-CBA. While, strain 4-CBA seemed to have a more compact gene cluster of fcbABC compared to previously reported strains. This study provides new insights into the catabolic diversity of structurally similar isomers in a contaminated niche. (C) 2017 Elsevier Ltd. All rights reserved.
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