Article
Biochemistry & Molecular Biology
Jiang Zhu, Ruinan Qu, Yawei Wang, Ruoyao Ni, Kai Tian, Chan Yang, Mei Li, Michael Kristensen, Xinghui Qiu
Summary: Many insect species have developed various resistance mechanisms in response to insecticide selection. This study focuses on understanding the molecular mechanisms of P450-mediated insecticide resistance using the house fly. The results highlight the common involvement of the CncC pathway in adaptive evolution and provide evidence for parallel evolution in P450-mediated insecticide resistance.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Agronomy
Pei-Rong Li, Yu Shi, Di Ju, Yu-Xi Liu, Wei Wang, Ying-Shi He, Yu-Yun Zhang, Xue-Qing Yang
Summary: The study found that functional redundancy and preference of metabolism by CYP9A genes in the CYP9A subfamily contribute to lambda-cyhalothrin resistance in Cydia pomonella. These genes are highly expressed in the larval midgut and are induced by lambda-cyhalothrin. Knockdown experiments and in vitro assays confirmed the metabolic roles of these genes in lambda-cyhalothrin. These findings reveal the molecular mechanism of P450-mediated lambda-cyhalothrin resistance and its adaptive evolutionary strategy in Cydia pomonella.
PEST MANAGEMENT SCIENCE
(2023)
Article
Agronomy
Shuirong Zhang, Fuchuan Gu, Ying Du, Xuyang Li, Changwei Gong, Jian Pu, Xuemei Liu, Xuegui Wang
Summary: Continuous selection of Laodelphax striatellus on triflumezopyrim resulted in the development of resistance, which did not lead to cross-resistance to other insecticides. The up-regulation of multiple P450 genes may play a role in mediating triflumezopyrim resistance in L. striatellus.
PEST MANAGEMENT SCIENCE
(2022)
Article
Parasitology
Xixi Li, Shengli Hu, Hongbo Zhang, Haitao Yin, Huan Wang, Dan Zhou, Yan Sun, Lei Ma, Bo Shen, Changliang Zhu
Summary: This study investigated the roles of miR-279-3p and its target gene CYP325BB1 in deltamethrin resistance in Culex pipiens pallens. The findings showed that miR-279-3p suppresses CYP325BB1 expression, reducing deltamethrin resistance and increasing mosquito mortality. These results provide important insights for future mosquito control strategies.
PARASITES & VECTORS
(2021)
Article
Entomology
Amir Zaman Shah, Chao Ma, Yuanyuan Zhang, Qiuxin Zhang, Gang Xu, Guoqing Yang
Summary: This study demonstrates that the use of decoyinine as a seed treatment significantly reduces the fecundity of the small brown planthopper (SBPH) and improves rice resistance. Decoyinine enhances rice defense against SBPH by stimulating plant defensive responses, making it a potential environmentally friendly alternative to chemical pest control.
Article
Agriculture, Multidisciplinary
Yixi Zhang, Baojun Yang, Zhiming Yang, Lu Kai, Zewen Liu
Summary: Two P450 enzymes, CYP4C62 and CYP6BD12, in Nilaparvata lugens were found to bioactivate chlorpyrifos into its active ingredient chlorpyrifos-oxon in vivo and in vitro. Knockdown of these two genes reduced sensitivity to chlorpyrifos and the formation of chlorpyrifos-oxon in N. lugens. This study revealed a novel mechanism of insecticide resistance due to the reduction in bioactivation.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Virology
Gang Lu, Xiao-Di Zhang, Zhong-Tian Xu, Zhuang-Xin Ye, Yan Zhang, Jian-Ping Chen, Chuan-Xi Zhang, Jun-Min Li
Summary: A novel reovirus-like virus named Laodelphax striatellus reovirus (LSRV) was identified in the small brown planthopper, L. striatellus. LSRV was widely distributed in various tissues of the planthopper, with the highest expression in adult heads. However, LSRV was unable to replicate in rice plants. Host antiviral RNA interference pathway was found to be involved in LSRV infection. LSRV may be considered as a new species of the genus Fijivirus in the order Reovirales.
Review
Entomology
Ralf Nauen, Chris Bass, Rene Feyereisen, John Vontas
Summary: Insect cytochrome P450 monooxygenases (P450s) play important physiological roles and are particularly involved in the detoxification of xenobiotics. Recent advances in insect genomics and functional approaches have shed light on the evolution of insect P450s, their regulation mechanisms, and the genetic alterations leading to insecticide resistance. Additionally, studies on the sensitivity of beneficial insects to insecticides have provided valuable knowledge for managing P450-mediated resistance and safeguarding their health.
ANNUAL REVIEW OF ENTOMOLOGY
(2022)
Article
Agronomy
Tianfei Peng, Xuemei Liu, Fayi Tian, Hongfei Xu, Fengting Yang, Xuewei Chen, Xiwu Gao, Yuntong Lv, Jianyi Li, Yiou Pan, Qingli Shang
Summary: The study revealed that the upregulation of certain cytochrome P450 genes is associated with spirotetramat resistance in A. gossypii, and some lncRNAs may regulate the expression of these P450 genes to affect resistance. These findings provide a molecular basis for further investigating cotton aphid P450 genes and lncRNA functions in spirotetramat resistance development.
PEST MANAGEMENT SCIENCE
(2022)
Article
Entomology
Jing Yang, Buli Fu, Peipan Gong, Chengjia Zhang, Xuegao Wei, Cheng Yin, Mingjiao Huang, Chao He, Tianhua Du, Jinjin Liang, Shaonan Liu, Yao Ji, Hu Xue, Chao Wang, Jinyu Hu, He Du, Rong Zhang, Xin Yang, Youjun Zhang
Summary: In this study, we investigated the role of CYP6CX2 and CYP6CX3 in mediating whitefly thiamethoxam resistance. Our results showed that the mRNA levels of these two genes were up-regulated after exposure to thiamethoxam. Transcriptional levels were overexpressed in resistant strains and positively correlated with resistance levels. Silencing these genes increased whitefly susceptibility to thiamethoxam. These findings provide insights into the role of cytochrome P450 monooxygenases in neonicotinoid resistance and suggest their potential as target genes for pest management.
JOURNAL OF ECONOMIC ENTOMOLOGY
(2023)
Article
Entomology
Wenjing Yu, Qingqing Ren, Lingyao Jin, Min Wu
Summary: The study uncovers the cross-resistance mechanism of the small brown planthopper to pyrethroids and identifies CYP4FB1 and CYP301B1 as the detoxification enzymes responsible for this resistance.
JOURNAL OF APPLIED ENTOMOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Yuanxue Yang, Aiyu Wang, Yun Zhang, Chao Xue, Ming Zhao, Jianhua Zhang
Summary: Frequent use of insecticide can lead to environmental hazards and insecticide resistance in pests. This research identifies three endogenous microRNAs that are necessary for the small brown planthopper to develop tolerance to triflumezopyrim, an insecticide. These microRNAs activate metabolic detoxification pathways and interact with specific genes and proteins involved in resistance. Understanding these mechanisms is crucial for delaying the development of insecticide resistance.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biochemistry & Molecular Biology
Xia Zhao, Xiang Xu, Xue-gui Wang, Yong Yin, Ming-yang Li, Ya-qiong Wu, Ying-hong Liu, Qing-hua Cheng, Chang-wei Gong, Li-tao Shen
Summary: The study found that populations of Chilo suppressalis in different regions of Sichuan Province, China, exhibited low to moderate resistance levels to three insecticides, with the XW19 showing a potential association between resistance to triazophos and cytochrome P450 monooxygenase. The overexpression of CYP324A12, CYP321F3, and CYP9A68 in resistant populations, particularly in XW19, suggests a possible link to resistance development in C. suppressalis.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2021)
Article
Parasitology
Xixi Li, Shengli Hu, Haitao Yin, Hongbo Zhang, Dan Zhou, Yan Sun, Lei Ma, Bo Shen, Changliang Zhu
Summary: This study revealed that miR-4448 is involved in deltamethrin resistance in mosquitoes by targeting CYP4H31, leading to decreased expression of CYP4H31. These findings contribute to a better understanding of the mechanisms underlying deltamethrin resistance.
PARASITES & VECTORS
(2021)
Article
Biochemistry & Molecular Biology
Melanie Nolden, Mark J. I. Paine, Ralf Nauen
Summary: Pyrethroid resistance in Anopheles funestus is a major threat to the eradication of malaria. This study identified the enzymes CYP6P9a and CYP6P9b as major drivers of pyrethroid resistance in An. funestus, and investigated their interactions with azole-fungicides and pyrethroids. The study found that the mutation V310I in CYP6P9b increased its metabolic activity towards pyrethroids, particularly permethrin. Additionally, the study discovered the first metabolites of permethrin and deltamethrin, which were also suitable substrates for the CYP6P9 variants and were depleted to a higher extent than their parent compounds. Furthermore, it was confirmed that CYP6P9a and CYP6P9b are capable of metabolizing pyrethroids. These findings provide new insights into the molecular mechanisms of pyrethroid resistance in An. funestus.
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2022)
Article
Entomology
Shi-Wei Liu, Mohammed Esmail Abdalla Elzaki, Christian Staehelin, Zhi-Hui Ma, Zhong Qin, Rui-Long Wang
Article
Biochemistry & Molecular Biology
Mohammad Asaduzzaman Miah, Mohammed Esmail Abdalla Elzaki, Asmaul Husna, Zhaojun Han
ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Cheng-Zhen Gu, Sheng-Hao Yuan, Jing Lu, Yi-Jun Qiao, Yuan-Yuan Song, Mohammed Esmail Abdalla Elzaki, Chong-Ren Yang, Ying-Jun Zhang, Ren-Sen Zeng
CHEMISTRY & BIODIVERSITY
(2019)
Article
Biochemistry & Molecular Biology
Shi Chen, Mohammed Esmail Abdalla Elzaki, Chaohui Ding, Zheng-fang Li, Jie Wang, Ren-sen Zeng, Yuan-Yuan Song
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Xiao-Long Liu, Si-Jie Sun, Sajjad Ali Khuhro, Mohammed Esmail Abdalla Elzaki, Qi Yan, Shuang-Lin Dong
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2019)
Article
Virology
Rui-Long Wang, Keyan Zhu-Salzman, Mohammed Esmail Abdalla Elzaki, Qiao-Qiao Huang, Shi Chen, Zhi-Hui Ma, Shi-Wei Liu, Jia-En Zhang
Article
Biochemistry & Molecular Biology
Mohammed Esmail Abdalla Elzaki, Rong-rong Xue, Lin Hu, Jin-da Wang, Ren-sen Zeng, Yuan-yuan Song
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2019)
Article
Agronomy
Z. H. Ma, M. E. A. Elzaki, K. J. Guo, C. X. Fang, Z. Qin, H. Y. Ji, Y. J. Su, R. L. Wang
ALLELOPATHY JOURNAL
(2019)
Article
Entomology
Qianqian Xiao, Lei Deng, Mohammed Esmail Abdalla Elzaki, Lin Zhu, Yanfei Xu, Xiangyu Han, Chunyu Wang, Zhaojun Han, Min Wu
JOURNAL OF ECONOMIC ENTOMOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Yingchuan Peng, Kangxu Wang, Guanheng Zhu, Qunxin Han, Jiasheng Chen, Mohammed Esmail Abdalla Elzaki, Chengwang Sheng, Chunqing Zhao, Subba Reddy Palli, Zhaojun Han
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2020)
Article
Engineering, Environmental
Bo Hu, Miaomiao Ren, Jianfeng Fan, Sufang Huang, Xia Wang, Mohammed Esmail Abdalla Elzaki, Chris Bass, Subba Reddy Palli, Jianya Su
JOURNAL OF HAZARDOUS MATERIALS
(2020)
Article
Ecology
Jie Wang, Rongrong Xue, Xueyang Ju, Hui Yan, Zhou Gao, Mohammed Esmail Abdalla Elzaki, Lin Hu, Rensen Zeng, Yuanyuan Song
ECOLOGY AND EVOLUTION
(2020)
Article
Engineering, Environmental
Mohammed Esmail Abdalla Elzaki, Zhen-fang Li, Jie Wang, Lu Xu, Nannan Liu, Ren-sen Zeng, Yuan-yuan Song
JOURNAL OF HAZARDOUS MATERIALS
(2020)
Article
Genetics & Heredity
Bo Hu, He Huang, Songzhu Hu, Miaomiao Ren, Qi Wei, Xiangrui Tian, Mohammed Esmail Abdalla Elzaki, Chris Bass, Jianya Su, Subba Reddy Palli
Summary: The overexpression of P450 gene CYP321A8 in the insect Spodoptera exigua, resistant to multiple insecticides, was identified as a major factor contributing to resistance. Two independent mechanisms, constitutive upregulation of transcription factors and mutations in the promoter region allowing for binding of an orphan nuclear receptor, were found to be involved in the overexpression of CYP321A8 and resistance in S. exigua.
Article
Biochemistry & Molecular Biology
Wen-Tao Hou, Christian Staehelin, Mohammed Esmail Abdalla Elzaki, Muhammad Hafeez, Yu-Sen Luo, Rui-Long Wang
Summary: Spodoptera litura is a widely distributed, highly polyphagous pest that has developed resistance to various insecticides. This study found that elevated cytochrome P450 monooxygenase enzyme activity, particularly the overexpression of the novel gene CYP6AE68, in populations with resistance to indoxacarb may play a significant role in detoxification processes.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2021)