4.7 Article

Alkaline deacetylation as a strategy to improve sugars recovery and ethanol production from rice straw hemicellulose and cellulose

Journal

INDUSTRIAL CROPS AND PRODUCTS
Volume 106, Issue -, Pages 65-73

Publisher

ELSEVIER SCIENCE BV
DOI: 10.1016/j.indcrop.2016.08.053

Keywords

Rice straw; Deacetylation; Dilute acid pretreatment; Hemicellulosic hydrolysate; SSF; Ethanol

Funding

  1. Fundacao de Amparo a Pesquisa do Estado de sao Paulo (FAPESP) of Brazil [2013/13953-6, 2015/24813-6]

Ask authors/readers for more resources

A mild alkaline pretreatment (deacetylation) prior to the dilute acid pretreatment was evaluated as a strategy to improve the sugars recovery and ethanol production from both hemicellulose and cellulose fractions of rice straw. This pretreatment was carried out using different conditions of temperature (50-70 degrees C) and NaOH loading (20-80 mg NaOH/g biomass), which were combined according to a 22 central composite design. In this step the removal of acetyl groups as well as the impact of this step on biomass composition were evaluated. In order to assess the impact of the deacetylation on hemicellulosic hydrolysate composition, the influence of the reaction time (30-90 min) and sulfuric acid concentration (0.5-1.5% w/v) was also studied, using the alkaline-pretreated solid (deacetylated) and rice straw in natura. The best sequential pretreatment conditions were scaled-up to 50-L reactor, being obtained a cellulose-rich pretreated solid (cellulignin) and a hemicellulosic hydrolysate, which was concentrated to 70 g/L xylose to be used as fermentation medium. A significant improvement on ethanol production from xylose by Scheffersomyces stipitis NRRL Y-7124 was observed when the biomass was submitted to deacetylation (about 4-fold). The enzymatic conversion of cellulose was also improved (from 73 to 89%) when the deacetylated cellulignin was used, resulting in an enhancement of the ethanol production (from 12.7 to 20.4g/L) during the simultaneous saccharification and fermentation with Kluyveromyces marxianus NRRL Y-6860. In brief, biomass deacetylation prior to dilute acid pretreatment was an efficient strategy for rice straw processing, substantially improving the ethanol production from both pentose and hexose sugars. (c) 2016 Elsevier B.V. All rights reserved.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.7
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

Article Multidisciplinary Sciences

Extrusion of process flavorings from methionine and dextrose using modified starch as a carrier

Sirinapa Sasanam, Benjawan Thumthanaruk, Sombat Wijuntamook, Vasan Rattananupap, Savitri I. Vatanyoopaisarn, Chureerat Puttanlek, Dudsadee Uttapap, Solange Mussatto, Vilai Rungsardthong

Summary: This study aimed to produce process flavorings using the Maillard reaction by extrusion method with methionine and glucose. A carrier, modified starch, was used to reduce torque during production. Different formulations with varying ratios of methionine, glucose, modified starch, and water were prepared and fed into an extruder. Parameters such as appearance, torque, pH, color, volatile compounds, and sensory evaluation were measured. The extrudate with the highest amount of modified starch (MS45) had the highest L* value of 88.00, increasing to 93.00 after grinding. All formulations showed similar sensory scores in terms of aroma, taste, and solubility, with slight differences in color. The torque varied among the formulations, with MS25, MS35, and MS45 having lower torque than MS5 and MS15. Gas chromatography-mass spectrometry analysis of the extruded flavorings from MS25 revealed the presence of various volatile compounds, including sulfurous and vegetable odor compounds. The successful production of process flavorings using modified starch as a carrier was demonstrated.

PLOS ONE (2023)

Article Food Science & Technology

Microbial meat: A sustainable vegan protein source produced from agri-waste to feed the world

Samara Cardoso Alves, Erick Diaz-Ruiz, Bruna Lisboa, Minaxi Sharma, Solange I. Mussatto, Vijay Kumar Thakur, Deepak M. Kalaskar, Vijai Kumar Gupta, Anuj K. Chandel

Summary: In the modern world, the sustainability of animal and plant protein is being called into question due to the need for arable land and potable water. Finding alternative protein sources for human consumption is urgent, especially in developing countries. Microbial bioconversion of valuable materials into nutritious microbial cells offers a sustainable alternative, known as microbial protein (SCP), which can be used for both human and animal food. Producing SCP not only provides a sustainable protein source but also helps reduce waste disposal problems and production costs. However, addressing challenges such as awareness and regulatory acceptance is crucial for SCP to become a viable alternative.

FOOD RESEARCH INTERNATIONAL (2023)

Article Biotechnology & Applied Microbiology

Valorizing industrial side streams through microalgae cultivation: A roadmap for process scale-up

Sandra Beatriz Castillo Castaneda, Rafael Emilio Altamirano Torres, Miguel Angel Dominguez Vizcaino, Elda M. Melchor-Martinez, Mariel Araceli Oyervides-Munoz, Juan Eduardo Sosa Hernandez, Roberto Parra-Saldivar, Thomas Bisgaard, Omid Tavakoli, Irini Angelidaki, Solange I. Mussatto, Eduardo Krebs Kleingesinds, Seyed Soheil Mansouri

Summary: Currently, there is a growing demand for more sustainable production processes and a shift towards zero-waste manufacturing. Microalgae have emerged as an alternative to traditional remediation techniques and have the advantage of producing highly valuable products. This review aims to provide guidance on the use of microalgae, including biomass productivity, removal of pollutants, and composition of microalgae biomass, to help academics and industrial stakeholders optimize and valorize their side-streams.

ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS (2023)

Article Biotechnology & Applied Microbiology

Solid feeding and co-culture strategies for an efficient enzymatic hydrolysis and ethanol production from sugarcane bagasse

Juliana P. Sandri, Julen Ordenana, Thais S. Milessi, Teresa C. Zangirolami, Solange I. Mussatto

Summary: This study focuses on a feeding strategy to enhance high solid load enzymatic hydrolysis of sugarcane bagasse and sugar co-fermentation using a non-recombinant yeast co-culture. The findings provide significant contribution to improving the feasibility of second-generation biomass energy production.

ENVIRONMENTAL TECHNOLOGY & INNOVATION (2023)

Article Biotechnology & Applied Microbiology

Bioconversion Study of Olive Tree Biomass Hemicellulosic Hydrolysates by Candida guilliermondii at Different Scales for Ethanol and Xylitol Production

Juan Gabriel Puentes, Soledad Mateo, Sebastian Sanchez, Ines C. Roberto, Alberto J. Moya

Summary: This study utilized hemicellulosic biomass from olive-tree pruning to produce a hemicellulosic hydrolysate for fermentation. The non-traditional yeast Candida guilliermondii FTI 20037 was used to obtain ethanol and xylitol. The research focused on studying relevant kinetic parameters and comparing different fermentation scenarios.

FERMENTATION-BASEL (2023)

Article Biotechnology & Applied Microbiology

Adaptive laboratory evolution of Bacillus subtilis to overcome toxicity of lignocellulosic hydrolysate derived from Distiller's dried grains with solubles (DDGS)

Jasper L. S. P. Driessen, Josefin Johnsen, Ivan Pogrebnyakov, Elsayed T. T. Mohamed, Solange I. Mussatto, Adam M. Feist, Sheila I. Jensen, Alex T. Nielsen

Summary: The tolerance of microorganisms to toxic compounds generated during biomass pretreatment is a major challenge in the cost-effective production of bio-based products from lignocellulose. The understanding of tolerance mechanisms is limited, making rational engineering difficult. In this study, adaptive laboratory evolution was used to obtain 20 tolerant strains of Bacillus subtilis, capable of utilizing Distiller's Dried Grains with Solubles-derived hydrolysate. These evolved strains showed improved growth and retained heterologous enzyme production using a medium based on 100% hydrolysate, while the starting strains showed minimal growth. Whole-genome resequencing revealed mutations in the global regulator codY in 15 of the 19 sequenced isolates, as well as mutations in genes related to oxidative stress and flagella function. Overall, tolerance adaptive laboratory evolution proved to be valuable for the utilization of lignocellulose.

METABOLIC ENGINEERING COMMUNICATIONS (2023)

Article Biotechnology & Applied Microbiology

Screening of yeast co-culture using crude hydrolysate for co-fermentation of pentose and hexose

Juliana P. Sandri, Thais S. Milessi, Teresa C. Zangirolami, Solange I. Mussatto

Summary: Co-culture of Saccharomyces cerevisiae and evolved Kluyveromyces marxianus strains was evaluated for the co-fermentation of mixed pentoses and hexoses. Reliable data from the screening confirmed the effectiveness of this approach for biomass-derived sugar assimilation, with aeration control being highlighted as crucial for xylose assimilation.

BIOFUELS BIOPRODUCTS & BIOREFINING-BIOFPR (2023)

Review Energy & Fuels

Impact of Product Diversification on the Economic Sustainability of Second-Generation Ethanol Biorefineries: A Critical Review

Vinicius P. Shibukawa, Lucas Ramos, Monica M. Cruz-Santos, Carina A. Prado, Fanny M. Jofre, Gabriel L. de Arruda, Silvio S. da Silva, Solange I. Mussatto, Julio C. dos Santos

Summary: The replacement of fossil-based products with renewable alternatives, such as second-generation ethanol, is a major research topic. However, second-generation biorefineries face challenges in pretreatment, hydrolysis, and biomass utilization, hindering their economic sustainability. This article discusses how product diversification in cellulosic biorefineries enhances their economic sustainability, providing stakeholders with important information for investing in 2G ethanol biorefineries and better planning future ventures.

ENERGIES (2023)

Article Agricultural Engineering

Extraction of hydroxycinnamic acids (ferulic and p-coumaric) from rice straw alkaline black liquor using Pluronic F-127 for potential applications in the cosmetics industry

Alana M. M. Maia, Adalberto Pessoa-Junior, Ines C. Roberto

Summary: In this study, ferulic acid (FA) and p-coumaric acid (pCA) were extracted from rice straw using an aqueous two-phase system (ATPS) based on the cloud point of surfactant/copolymer Pluronic F-127 (PF-127). The extraction process resulted in 75.8% yield for FA and 74.3% yield for pCA at a concentration of 9.4% w/w of PF-127. Furthermore, the extracted FA and pCA were concentrated 2.3 and 2.5-fold, respectively, compared to their original concentrations, resulting in a PF-127 gel containing 0.12% w/w of hydroxycinnamic acids (HAs). This material shows potential for application in the cosmetics industry for high value-added products in rice straw-based biorefineries.

INDUSTRIAL CROPS AND PRODUCTS (2023)

Article Biochemistry & Molecular Biology

Enzymatic hydrolysis cocktail optimization for the intensification of sugar extraction from sugarcane bagasse

Eva Balaguer Moya, Berta Syhler, Julen Ordenana Manso, Giuliano Dragone, Solange I. Mussatto

Summary: This study evaluated the optimization of enzymatic hydrolysis cocktail to maximize sugar extraction from pretreated sugarcane bagasse. It was found that adding hydrogen peroxide at the beginning of hydrolysis increased glucose and xylose concentrations, while the addition of hemicellulase increased glucose and xylose production. These findings suggest that using an appropriate enzymatic cocktail supplemented with additives can enhance sugar extraction from lignocellulosic biomass and contribute to the development of a more sustainable and competitive process.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2023)

Article Green & Sustainable Science & Technology

Reduced-pressure alkaline pretreatment as an innovative and sustainable technology to extract protein from brewer's spent grain

Yasmim Arantes da Fonseca, Leandro Vinicius Alves Gurgel, Bruno Eduardo Lobo Baeta, Giuliano Dragone, Solange I. Mussatto

Summary: This study investigated the reduced-pressure alkaline pretreatment (RPAP) as an innovative approach for efficient protein extraction from lignocellulosic biomass. Optimal conditions were determined to achieve high protein yield, selectivity, and environmental sustainability. This research opens up new opportunities for the valorization of protein-rich lignocellulosic materials in biorefineries.

JOURNAL OF CLEANER PRODUCTION (2023)

Article Multidisciplinary Sciences

Improving Saccharomyces cerevisiae acid and oxidative stress resistance using a prokaryotic gene identified by functional metagenomics

Luana de Fatima Alves, Jonata Bortolucci, Valeria Reginatto, Maria-Eugenia Guazzaroni, Solange I. Mussatto

Summary: Innovations in obtaining products from lignocellulosic biomass have focused on improving microorganisms and enzymes for degrading these materials. To complete the process, microorganisms must ferment sugars and withstand various stresses. In this study, we engineered Saccharomyces cerevisiae strains to enhance acid and oxidative stress resistance by introducing a gene (hu) and using different promoters. The strains showed improved survival rates at low pH and increased tolerance to high concentrations of H2O2.

HELIYON (2023)

Article Biotechnology & Applied Microbiology

Dry Anaerobic Digestion of Brewer's Spent Grains toward a More Sustainable Brewery: Operational Performance, Kinetic Analysis, and Bioenergy Potential

William Gustavo Sganzerla, Josiel Martins Costa, Miriam Tena-Villares, Luz Selene Buller, Solange I. Mussatto, Tania Forster-Carneiro

Summary: This study evaluated the dry anaerobic digestion of brewer's spent grains (BSG) for bioenergy recovery as a solution toward a more sustainable brewery. The experimental results showed high biodegradation and methane yield, with the Cone model fitting the methane evolution better than the other models. The biogas produced from the dry AD of BSG could generate electricity and heat, mitigating CO2 emissions and supplying a portion of the energy required for beer production.

FERMENTATION-BASEL (2023)

Article Biotechnology & Applied Microbiology

Intensification of corn fiber saccharification using a tailor made enzymatic cocktail

Julen Ordenana Manso, Martin B. Nielsen, Eva Balaguer Moya, Juliana P. Sandri, Celina K. Yamakawa, Solange I. Mussatto

Summary: This study focuses on the development of an enzymatic cocktail for the conversion of corn fiber into monosaccharides. The results reveal that the recalcitrance and unique chemical composition of corn fiber require a tailored enzymatic cocktail for degradation, and attention should also be given to the pretreatment step.

ENZYME AND MICROBIAL TECHNOLOGY (2024)

Article Biochemistry & Molecular Biology

Enzyme-constrained metabolic model and in silico metabolic engineering of Clostridium ljungdahlii for the development of sustainable production processes

Antonio Caivano, Wouter van Winden, Giuliano Dragone, Solange I. Mussatto

Summary: Constraint-based genome-scale models (GEMs) are a powerful tool for predicting and analyzing microbial phenotypes. This study implemented enzymatic constraints into a GEM of Clostridium ljungdahlii, improving the predictive ability of growth rate and product profile. In silico metabolic engineering was then performed to enhance the production of desired fermentation products, resulting in different strategies for overproduction without redundant knockouts. The study highlights the potential of mixotrophic growth for improving cell growth and productivity with CO2 fixation.

COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL (2023)

Article Agricultural Engineering

Structural changes of poplar lignin during the ternary deep eutectic solvent (DES) treatment and synergetic alkali-DES treatment

Jingmo Zhou, Shubin Wu, Ying Liu

Summary: Expenditure isolation of lignin and the understanding of its structural variations during different isolation processes are crucial for the high-value utilization of lignin. This study proposed a green deep eutectic solvent (DES) consisting of choline chloride, ethylene glycol, and 4-chlorobenzene sulfonic acid for rapid lignin separation from poplar. The structural variations of DES lignin and its relationship with alkali pre-extraction of hemicellulose were investigated. The results showed that alkali pretreatment enhanced the DES separation of lignin and promoted the cleavage of specific linkages, resulting in reduced molecular weight and thermal stability of isolated DES lignin. Moreover, DES lignin obtained from integrated utilization displayed higher purity and S/G ratio compared to lignin obtained from direct DES treatment.

INDUSTRIAL CROPS AND PRODUCTS (2024)

Article Agricultural Engineering

Integrated metabolomic and transcriptomic analysis provides insights into the flavonoid formation in different Glycyrrhiza species

Yuping Li, Ziyan Xie, Yun Huang, Jiangyi Zeng, Chao Yang, Ling Yuan, Ying Wang, Yongqing Li

Summary: In this study, the characteristic flavonoids (CFs) of three licorice species were identified through integrated metabolic and transcriptomic analysis. The key regulators of CF biosynthesis were also identified. These findings provide valuable information for understanding the biosynthesis of CFs and future breeding of Glycyrrhiza varieties.

INDUSTRIAL CROPS AND PRODUCTS (2024)

Article Agricultural Engineering

Construction of robust and fully bio-based epoxy vitrimers with facile malleability, self-healing ability, and recyclability from peach gum polysaccharide and itaconic acid

Xianjie Pan, Zhaoyi Luo, Fanqi Liu, Quanqian Yi, Yanning Zeng

Summary: This study presents a novel strategy for fabricating fully bio-based epoxy vitrimers using peach gum and itaconic acid, which exhibit superior mechanical properties and thermostability. The materials were characterized and the self-healing and recycling capabilities were evaluated. The results demonstrate the potential of using these materials for sustainable industrial applications.

INDUSTRIAL CROPS AND PRODUCTS (2024)

Article Agricultural Engineering

Colonization of Satureja hortensis L. (Summer savory) with Trichoderma harzianum alleviates salinity stress via improving physio-biochemical traits and biosynthesis of secondary metabolites

Mohsen Sabzi-Nojadeh, Mohammad Pouresmaeil, Mina Amani, Mehdi Younessi-Hamzekhanlu, Filippo Maggi

Summary: This study shows that inoculating Summer savory plants with endophytic fungus Trichoderma harzianum can improve plant growth and increase salt tolerance. The fungus enhances growth, restores chlorophyll and antioxidant levels, reduces harmful substances, and increases proline and soluble sugar levels, thereby increasing crop productivity in saline conditions.

INDUSTRIAL CROPS AND PRODUCTS (2024)

Article Agricultural Engineering

Sustainable fragrance release wax oil coating for wood substrate based on peppermint essential oil microcapsules

Yu Liu, Lei Li, Aichen Zhao, Xiuming Song, Lulu Wei, Mengdi Fang, Chuchu Zheng, Xiaodong Zhu

Summary: This study investigates the impact of peppermint essential oil microcapsules on the unpleasant odor emissions from wax oil coated wood products. The results show that microcapsules can improve the time and stability of odor release.

INDUSTRIAL CROPS AND PRODUCTS (2024)

Article Agricultural Engineering

Land suitability assessment for rapeseed potential cultivation in upper Tigris basin of Turkiye comparing fuzzy and boolean logic

Mesut Budak, Mirac Kilic, Hikmet Guenal, Ismail Velik, Mesut Sirri

Summary: This study assessed land suitability for rapeseed production using topography, climate, and soil data by analytical hierarchy process (AHP) and the Mamdani Fuzzy Inference System (MFIS). The study area covered land in the Diyarbakir province of southeastern Turkiye. The results indicated the proportions of different land suitability classes and showed the compatibility of AHP and MFIS methods in assessing suitability.

INDUSTRIAL CROPS AND PRODUCTS (2024)

Article Agricultural Engineering

Strong, UV-blocking, and hydrophobic PVA composite films containing tunable lignin nanoparticles

Rui Tian, Yue Liu, Chao Wang, Weikun Jiang, Srinivas Janaswamy, Guihua Yang, Xingxiang Ji, Gaojin Lyu

Summary: In this study, hollow and solid lignin nanoparticles were synthesized through self-assembly and their application in PVA nanocomposite films was investigated. The results showed that the presence of hollow lignin nanoparticles in the films resulted in a room-like network structure, while the solid lignin nanoparticles induced different structural changes. The inclusion of LNPs improved the mechanical properties of the films, and both types of LNPs exhibited good hydrophobicity.

INDUSTRIAL CROPS AND PRODUCTS (2024)

Article Agricultural Engineering

Alternative for natural sweeteners: Improving the use of stevia as a source of steviol glycosides

Marcos Vinicius Silva de Andrade, Simone Ribeiro Lucho, Renato Delmondez de Castro, Paulo Roberto Ribeiro

Summary: Stevia rebaudiana Bertoni, commonly known as stevia, is a widely cultivated shrub with highly sweetening metabolites. It has gained significant interest in the food and beverage industries due to its extremely sweet taste and various health benefits. Researchers are currently focused on studying biotechnological strategies to enhance the biosynthesis of stevia plant metabolites for improved cultivation and production.

INDUSTRIAL CROPS AND PRODUCTS (2024)

Article Agricultural Engineering

Characterization of an Arundo donax-based composite: A solution to improve indoor comfort

Eleonora Cintura, Paulina Faria, Luisa Molari, Luca Barbaresi, Dario D'Orazio, Lina Nunes

Summary: This study investigated the properties of Arundo donax as a building material and found that it has strong adaptability, high hygroscopicity, and unique sound absorption behavior. The mechanical tests also demonstrated the applicability of the Arundo donax-based composite. This opens up new possibilities for the further application of Arundo donax-based materials.

INDUSTRIAL CROPS AND PRODUCTS (2024)

Article Agricultural Engineering

The morphology, inflorescence yield, and secondary metabolite accumulation in hemp type Cannabis sativa can be influenced by the R:FR ratio or the amount of short wavelength radiation in a spectrum

Stiina Kotiranta, Juha-Matti Pihlava, Titta Kotilainen, Pauliina Palonen

Summary: The effect of light spectra, particularly far-red and short wavelength radiation, on the growth and secondary metabolites of cannabis is significant. Altering the R:FR ratio can increase the concentration and yield of secondary metabolites, while short wavelength radiation does not significantly affect plant morphology and secondary metabolite concentrations.

INDUSTRIAL CROPS AND PRODUCTS (2024)

Article Agricultural Engineering

Investigation of a Na2S-assisted deep eutectic solvent pretreatment for enhanced cellulose/xylan saccharification and high-quality lignin with abundant ether bond

Xuze Liu, Fangmin Liang, Jinyuan Cheng, Xuelian Zhou, Jia Wang, Guigan Fang, Kuizhong Shen, Xianzhi Meng, Chen Huang, Arthur J. Ragauskas

Summary: A novel Na2S-facilitated DES pretreatment method was proposed to fractionate moso bamboo, which achieved high lignin removal and preserved most cellulose and hemicellulose with excellent enzymatic digestibility.

INDUSTRIAL CROPS AND PRODUCTS (2024)

Article Agricultural Engineering

Advancing multi-stimuli 4D printing of polylactic acid with rapid shape memory and enhanced toughness using lignin-coated Fe3O4 nanoparticles

Zechun Ren, Xinyuan Zhou, Kejiao Ding, Tong Ji, Hao Sun, Xiang Chi, Yunzhao Wei, Min Xu

Summary: This study prepared a thermal/magnetic/light-triggered multi-stimuli responsive shape memory polymer composite with excellent properties by adding magnetic nanoparticles into a polyurethane composite. The composite showed high shape recovery rate, fast response behavior, and suitability for 3D printing. Controllable external stimuli allowed for dynamic, remote, and precise control of 4D transformations. This work is significant for the design of smart materials and devices.

INDUSTRIAL CROPS AND PRODUCTS (2024)

Article Agricultural Engineering

Mathematical models for predicting the higher heating value and ultimate analysis of biomass

Yurany Camacho Ardila, Jaiver Efren Jaimes Figueroa, Maria Regina Wolf Maciel

Summary: The higher heating value (HHV) and elemental composition are important properties of biomass, and their prediction using proximate and ultimate analysis is accurate.

INDUSTRIAL CROPS AND PRODUCTS (2024)

Article Agricultural Engineering

Three-dimensional structural characterization and mechanical properties of bamboo parenchyma tissue

Xiaohan Chen, Xianke Wang, Lili Shang, Xinxin Ma, Changhua Fang, Benhua Fei, Huanrong Liu, Shuqin Zhang

Summary: This study focuses on the comprehensive characterization of the three-dimensional (3D) structure of bamboo parenchyma and the evaluation of its tensile and compressive properties, as well as the analysis of its failure mechanisms. The study found that the measured tensile strength and elastic modulus of the parenchyma tissue are 48.81 MPa and 706.48 MPa, respectively. Compression failure modes in parenchyma tissue encompass compression bending and cell fracture, shear failure of the middle lamella, and detachment and debonding of the cell wall.

INDUSTRIAL CROPS AND PRODUCTS (2024)

Article Agricultural Engineering

Structural, defense enzyme activity and chemical composition changes in the xylem of Aquilaria sinensis during fungus induction

Tianfeng Liu, Yuxin Liu, Yunlin Fu, Mengji Qiao, Penglian Wei, Zhigao Liu, Yingjian Li

Summary: This study explored the formation mechanism of agarwood induced by fungus and its potential application in high-quality and high-yielding agarwood production. The results showed that the transition layer played a critical role in the continuous accumulation of agarwood, and the parenchyma cells of the xylem rays and interxylary phloem were the main action site of fungus. The accumulation and distribution of chemical composition in different layers were also investigated. The study identified the drivers of specific compounds and provided a theoretical foundation for future agarwood production.

INDUSTRIAL CROPS AND PRODUCTS (2024)