Journal
INDUSTRIAL CROPS AND PRODUCTS
Volume 95, Issue -, Pages 170-174Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.indcrop.2016.10.019
Keywords
Cellulose; Soy protein isolate; Hybrid; Calcium phosphate; Mineralization
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Funding
- National Research Center, Cairo, Egypt [P100108]
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The current study is a new approach to prepare an environmentally frieridly and bioactive material from cellulose grafted soy protein isolate (SPI) for biomimetic calcium phosphate mineralization. Neat cellulose was oxidized using periodate then chemically modified by reacting with SPI followed by soaking in doubly concentrated simulated body fluid (2xSBF) solution. Scanning electron microscopy/energy dispersive Xray spectroscopy, X-ray diffraction and transmission electron microscopy suggested the formation of uniform hydroxyapatite rod-like nanocrystals with similar to 50 nm diameter. The chemical composition of the prepared materials was further investigated using infrared spectroscopy and thermogravimetric analysis. The cytotoxicity of cellulose/SPI/calcium phosphate hybrid was evaluated using animal fibroblast baby hamster kidney cells (BFIK-21). The cytotoxicity results suggested cellulose/SPI/calcium phosphate hybrid as potentially useful scaffold for regenerative therapies. The current article suggests a green strategy for preparing a new biohybrid material for tissue engineering. (C) 2016 Elsevier B.V. All rights reserved.
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