Journal
INDIAN JOURNAL OF MICROBIOLOGY
Volume 57, Issue 3, Pages 365-369Publisher
SPRINGER
DOI: 10.1007/s12088-017-0665-1
Keywords
Colony PCR; DNA extraction; High throughput screening; Sonication; Sequencing
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Funding
- Ministry of Trade, Industry and Energy (MOTIE, Korea) [N042600010]
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Current colony PCR methods are not suitable for screening genes encoded in genomic DNA and are limited to E. coli host strains. Here, we describe an ultra-high efficient colony PCR method for high throughput screening of bacterial genes embedded in the genomic DNA of any bacterial species. This new technique expands colony PCR method to several hosts as well as offers a rapid, less expensive and reliable bacterial genomic DNA extraction.
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