Article
Biochemistry & Molecular Biology
Yoshimi Shimizu, Takehiro Shinoda, Yoshitaka Shirasago, Masuo Kondoh, Naoko Shinya, Kentaro Hanada, Kiyohito Yagi, Tetsuro Suzuki, Takaji Wakita, Tomomi Kimura-Someya, Mikako Shirouzu, Masayoshi Fukasawa
Summary: The study demonstrates that the conversion of monoclonal antibodies to monovalent antigen-binding fragments and single-chain variable fragment antibodies maintains high binding specificity and affinity, and also shows inhibition of HCV infection. These functional monovalent OCLN-binding probes have potential as drug candidates and tools for studies of OCLN in biology and pharmaceuticals.
Article
Biochemistry & Molecular Biology
Chihiro Aikawa, Kiyosumi Kawashima, Chihiro Fukuzaki, Makoto Nakakido, Kazunori Murase, Takashi Nozawa, Kouhei Tsumoto, Ichiro Nakagawa
Summary: Group A Streptococcus causes various diseases, with toxins like streptolysin O playing a key role in severe infections. Research has identified an anti-SLO single-chain variable fragment, SLO-I4, which effectively inhibits GAS-induced cell lysis and may be beneficial in combating GAS infections.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Medicine, Research & Experimental
Somayeh Piri-Gavgani, Mostafa Ghanei, Abolfazl Fateh, Seyed Davar Siadat, Leila Nematollahi, Fatemeh Rahimi-Jamnani
Summary: The research demonstrates that SP192 and SP220 are antibodies that can effectively bind to alpha-hemolysin, neutralize its toxicity, and significantly inhibit alpha-hemolysin-mediated lysis.
IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES
(2022)
Article
Fisheries
Chuang Cui, Liangyin He, Xiaoqian Tang, Jing Xing, Xiuzhen Sheng, Heng Chi, Wenbin Zhan
Summary: In this study, eight monoclonal antibodies (mAbs) against VP28 of WSSV were developed, and three of them were determined to have the ability to delay mortality in WSSV-infected shrimp. These mAbs specifically reacted with native VP28 of WSSV and recognized the virions in the gills of infected shrimp. Three biologically active single chain variable fragment (scFv) recombinant proteins were produced and capable of recognizing native VP28 and delaying the mortality of WSSV-infected shrimp. Eukaryotic expression plasmids of scFv genes were constructed and partially neutralized WSSV infection. Therefore, the production of neutralizing mAbs and recombinant scFv antibodies against WSSV shows promising therapeutic potential in preventing and treating white spot disease of shrimp.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Immunology
Kai-Thomas Schneider, Toni Kirmann, Esther Veronika Wenzel, Jan-Hendrik Grosch, Saskia Polten, Doris Meier, Marlies Becker, Paul Matejtschuk, Michael Hust, Giulio Russo, Stefan Duebel
Summary: Generation of sequence defined antibodies from universal libraries by phage display has been established as a robust method. ScFv-Fcs can be effective as neutralizing antibodies against a panel of pathogens and toxins, but may have variable stability. A generic lyophilization protocol has been developed to improve their shelf life, showing preservation of initial properties without compromising binding activity after storage.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Oncology
Shima Shabani, Mehdi Forouzandeh Moghadam, Seyed Latif Mousavi Gargari
Summary: In this study, a single-chain variable domain (scFv) specific for the C-terminal domain of recombinant human GRP78 was isolated using ribosome display technique. The selected scFv, GSF3, exhibited high affinity to CGRP and potential for cancer immunotherapy and diagnosis. The findings suggest that ribosome display is a reliable method for specific and high-affinity scFv isolation, with GSF3 showing promise for further evaluation in preclinical and clinical settings.
Article
Radiology, Nuclear Medicine & Medical Imaging
Philipp Diebolder, Cedric Mpoy, Jalen Scott, Truc T. Huynh, Ryan Fields, Dirk Spitzer, Nilantha Bandara, Buck E. Rogers
Summary: Glycoprotein CD44 and its splice variants are overexpressed in many cancers and cancer stem cells, with hyaluronic acid binding to CD44 to activate cell signaling pathways. Developing a CD44-specific antibody fragment for imaging CD44-positive cancers using PET shows potential clinical utility in cancer therapy.
JOURNAL OF NUCLEAR MEDICINE
(2021)
Article
Immunology
Chia-Yu Chang, Yong-Sheng Wang, Jou-Fei Wu, Tzu-Jing Yang, Yen-Chen Chang, Chanhee Chae, Hui-Wen Chang, Shang-Te Danny Hsu
Summary: The emergence of the G2 genotype and re-emergence of G1 porcine epidemic diarrhea virus have caused severe economic impacts, and the development of new variant vaccines has been challenging. The scFv specific to G2b PEDV could potentially be used as a therapeutic candidate against the virulent PEDV.
Article
Biochemical Research Methods
Manman Lin, Yuan Liu, Xiao Zhang, Jianfeng Zhong, Xiaodan Hu, Chongxin Xu, Yajing Xie, Cunzheng Zhang, Ying Liang, Xianjin Liu, Johnson Lin
Summary: The study obtained seven anti-Cry2Aa toxin anti-idiotypic scFv antibodies from a phage-displayed mutant library, with mutant 2-12B showing the best binding ability. 2-12B and Cry2Aa toxin shared a potential receptor of polycalin in P. xylostella BBMVs and had seven common binding amino acid residues in polycalin. Insect bioassay results indicated that 2-12 had insecticidal efficacy against P. xylostella larvae, suggesting that the 2-12B antibody partially mimics the structure and function of Cry2Aa toxin.
ANALYTICAL BIOCHEMISTRY
(2021)
Article
Fisheries
Siyou Huang, Yujia Wu, Lianpan Su, Taowen Su, Qiong Zhou, Jing Zhang, Zhiying Zhao, Shaoping Weng, Jianguo He, Junfeng Xie
Summary: In this study, an anti-NNV strategy using a single-chain variable fragment (ScFv) antibody named Sc3F9 was applied to eliminate the possibility of NNV infection during fertilization. The antibody showed broad neutralizing capacity and stable use in seawater, making it suitable for NNV decontamination, especially in obtaining NNV-free grouper eggs. Furthermore, the antibody demonstrated strong neutralization of OGNNV.
Review
Oncology
Paola Munoz-Lopez, Rosa Maria Ribas-Aparicio, Elayne Irene Becerra-Baez, Karla Fraga-Perez, Luis Fernando Flores-Martinez, Armando Alfredo Mateos-Chavez, Rosendo Luria-Perez
Summary: Recombinant antibody fragments, particularly the single-chain fragment variable (scFv), have shown great potential in cancer diagnosis and therapy with their high specificity, affinity, and ability to penetrate tumor tissues. They offer a promising alternative for patients who fail to respond to conventional treatments.
Article
Medicine, Research & Experimental
Sanne A. M. van Lith, Fokko J. Huizing, Gerben M. Franssen, Bianca A. W. Hoeben, Jasper Lok, Sofia Doulkeridou, Otto C. Boerman, Martin Gotthardt, Paul M. P. van Bergen en Henegouwen, Johan Bussink, Sandra Heskamp
Summary: This study compared variants of CAIX-binding VHH B9 with different affinities (low and high) for SPECT imaging of CAIX expression. The results showed that B9 variants with ABD demonstrated better tumor uptake, but some uptake was CAIX-independent, limiting their use in hypoxia assessment.
MOLECULAR PHARMACEUTICS
(2022)
Article
Biochemistry & Molecular Biology
Karin N. Westlund, Marena A. Montera, Aleyah E. Goins, Sascha R. A. Alles, Nikita Suri, Sabrina L. McIlwrath, Robyn Bartel, Ravi V. Durvasula, Adinarayana Kunamneni
Summary: Non-opioid engineered scFv antibodies targeting the P2X4R receptor show potential as biotherapeutic interventions for chronic pain, with enhanced binding affinity and tissue penetrability leading to persistent pain relief in animal models.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Cell Biology
Yan Yang, Guangming Zhu, Li Yang, Yun Yang
Summary: This article reviews the role of CD24 in tumorigenesis and cancer progression, its potential as a therapeutic target, and summarizes different therapeutic agents or strategies targeting CD24 in solid cancers.
CELL COMMUNICATION AND SIGNALING
(2023)
Article
Neurosciences
Sen Li, Yushan Yi, Ke Cui, Yanqiu Zhang, Yange Chen, Dou Han, Ling Sun, Xiaohui Zhang, Fei Chen, Yixin Zhang, Yufeng Yang
Summary: A new potential therapeutic agent against AD, the scFv T1 antibody, was screened in this study. It showed strong ability to inhibit tau aggregation in dilute solutions and under conditions of macromolecular crowding, and could depolymerize preformed tau aggregates in vitro, indicating its potential for treating neurodegenerative diseases.
JOURNAL OF ALZHEIMERS DISEASE
(2021)
Article
Immunology
Fatemeh Abarghooi-Kahaki, Abbas Basiri, Mojgan Bandehpour, Bahram Kazemi
IMMUNOLOGY LETTERS
(2019)
Article
Public, Environmental & Occupational Health
Leila Masoori, Reza Falak, Kobra Mokhtarian, Mojgan Bandehpour, Elham Razmjou, Nahid Jalallou, Farzane Jafarian, Lame Akhlaghi, Ahmad Reza Meamar
TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE
(2019)
Article
Biochemistry & Molecular Biology
Armina Alagheband Bahrami, Mojgan Bandehpour, Bahman Khalesi, Bahram Kazemi
INTERNATIONAL JOURNAL OF PEPTIDE RESEARCH AND THERAPEUTICS
(2020)
Article
Oncology
Roshanak Shams, Mahnaz Seifi-Alan, Mojgan Bandehpour, Mir Davood Omrani, Soudeh Ghafouri-Fard
PATHOLOGY & ONCOLOGY RESEARCH
(2020)
Article
Biochemistry & Molecular Biology
Amin Barkhordari, Abbas Behzad-Behbahani, Vahid Jajarmi, Mojgan Bandehpour, Gholamreza Rafiei-Dehbidi, Fatemeh Safari, Fereydoun Mahboudi, Bahram Kazemi
INTERNATIONAL JOURNAL OF PEPTIDE RESEARCH AND THERAPEUTICS
(2020)
Article
Biochemistry & Molecular Biology
Akram Jalali, Mojgan Bandehpour, Rouzbeh Chegeni, Hossein Ghanbarian, Yasaman Mardani-Dashti, Sayyed Hamid Zarkesh-Esfahani, Bahram Kazemi
Summary: Using Leishmania tarentolae as an expression system for producing therapeutic recombinant proteins is cost-effective, efficient, and requires less labor.
INTERNATIONAL JOURNAL OF PEPTIDE RESEARCH AND THERAPEUTICS
(2021)
Article
Biotechnology & Applied Microbiology
Reyhaneh Hoseinpoor, Bahram Kazemi, Masoumeh Rajabibazl, Azam Rahimpour
JOURNAL OF BIOTECHNOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Nasrin Mohammadi, Mojgan Bandehpour, Fattah Sotoodehnejadnematalahi, Bahram Kazemi
Summary: The study successfully designed and expressed the Ecarin metalloproteinase, and verified its enzyme activity through experiments. Combining the predictive results from the I-TASSER server, it helped to better understand the relationship between the structure and function of the Ecarin metalloproteinase.
PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS
(2022)
Article
Pharmacology & Pharmacy
Aref Shiralipour, Babak Khorsand, Leila Jafari, Mohammad Salehi, Mahsa Kazemi, Javad Zahiri, Vahid Jajarmi, Bahram Kazemi
Summary: This study analyzed the problem of drug resistance in breast cancer using computational and systems biology approaches. Six key lysosome-related genes were identified as potential therapeutic targets for drug-resistant breast cancer.
IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH
(2022)
Article
Chemistry, Physical
Maryam Rad, Gholamhossein Ebrahimipour, Mojgan Bandehpour, Omid Akhavan, Fatemeh Yarian
Summary: This study synthesized gold nanoparticles and investigated their ability to bind to single-chain variable fragment antibodies. The results showed that the gold nanoparticles can bind to the antibodies, highlighting their potential application in designing rapid diagnostic tests to identify infectious microorganisms promptly.
Article
Plant Sciences
Elnaz Bafandeh, Farnaz Kheirandish, Zeinab Hosseini, Saeed Veiskarami, Marzieh Rashidipour, Bahram Kazemi, Atiyeh Darbandi, Shirzad Fallahi, Arian Karimi Rouzbahani
Summary: Olive leaf extract (OLE) can significantly reduce the expression of DHFR gene and have a slight effect on the expression of PTR1 gene in Leishmania major parasite.
SOUTH AFRICAN JOURNAL OF BOTANY
(2023)
Article
Infectious Diseases
Farideh Tohidi, Zahra Babaei, Bahram Kazemi, Mojgan Bandehpour, Iraj Sharifi, Mohammad Reza Rabiei, Ebrahim Saedi Dezaki
JOURNAL OF VECTOR BORNE DISEASES
(2019)
Article
Biochemistry & Molecular Biology
H. Sobati, A. Dalimi, B. Kazemi, F. Ghaffarifar
MOLECULAR GENETICS MICROBIOLOGY AND VIROLOGY
(2019)
Article
Cell Biology
Alaleh Ghazifard, Mohammad Salehi, Marefat Ghaffari Novin, Mojgan Bandehpour, Somayeh Keshavarzi, Vahid Fallah Omrani, Maryam Dehghani-Mohammadabadi, Reza Masteri Farahani, Ahmad Hosseini