Journal
IMMUNOLOGIC RESEARCH
Volume 65, Issue 6, Pages 1156-1163Publisher
HUMANA PRESS INC
DOI: 10.1007/s12026-017-8966-3
Keywords
Dendritic cell; CD4(+) T lymphocyte; Salbutamol; beta 2-adrenergic receptor; IL-10
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Adrenergic receptor agonists and antagonists are extensively used as drugs in medicine for a broad spectrum of indications. We examined the consequences of beta 2-adrenergic stimulation of murine dendritic cells (DCs) on CD4(+) T cell activation. We demonstrated in vitro that treatment of LPS-matured DCs with the beta 2-agonist salbutamol reduced their ability to trigger OT-II T cell proliferation specific for ovalbumin antigen. Salbutamol also induced a decrease in MHC class II molecule expression by DC through Gi protein activation. Co-culture of CD4(+) T cells with salbutamol-conditioned mature DC impaired TNF alpha and IL-6 secretion while preserving IL-10 production by T cells. Using a vaccination protocol in mice, we showed that salbutamol favored IL-10-producing CD4(+) T cells. None of these effects was observed when working with beta 2-adrenoreceptor deficient mice. Finally, we suggest that beta 2-adrenergic stimulation of DC could be an interesting way to shape CD4(+) T cell responses for the purposes of immunotherapy.
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