Journal
CRUSTACEANA
Volume 88, Issue 10-11, Pages 1065-1078Publisher
BRILL
DOI: 10.1163/15685403-00003480
Keywords
14-3-3 gene; Scylla paramamosain; salinity adaption
Categories
Funding
- major science and technology agricultural projects of Zhejiang province [2008C12008]
- application research funds for non-profit projects of Zhejiang province [2013C32052]
Ask authors/readers for more resources
Two full-length cDNA sequences of 14-3-3 genes were isolated from the mud crab, Scylla paramamosain Estampador, 1949, using RT-PCR and RACE (rapid-amplification of cDNA ends). Sequence analysis indicates that both 14-3-3 genes contain an open reading frame of 744 bp with a deduced 247-amino-acid protein. The gene and protein sequences of Sp14-3-3 genes show 96 and 97% identity, respectively, and both Sp14-3-3 cluster together with other animal 14-3-3 proteins in phylogenetic tree analysis. Tissue specific expression analysis reveals that both Sp14-3-3 genes are ubiquitously expressed, however, their expression patterns are different from each other. Expressions of both Sp14-3-3 genes are sensitive to salinity decrease, even a 5 ppt drop from 30 ppt to 25 ppt, indicating that Sp14-3-3 genes are involved in osmoregulation. Our observations may contribute to a better understanding of the molecular and functional evolution of the 14-3-3 family in both crustaceans and (in) vertebrates as a whole.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available