Article
Microbiology
Kaixuan Huang, Yanqing Zhuang, Zhou Wang, Linjian Ou, Jingyi Cen, Songhui Lu, Yuzao Qi
Summary: Karenia mikimotoi is a well-known harmful bloom species in temperate coastal waters, and its characteristics in hydrolysis of different forms of phosphorus substrates, growth, and physiological responses were investigated in this study. The algae produced comparable quantities of enzymes to hydrolyze organic phosphorus substrates, with faster hydrolysis of phosphomonoesters compared to phosphodiesters. The presence of organic phosphorus substrates significantly influenced the hemolytic activities of K. mikimotoi, suggesting their potential important role in blooms.
Article
Plant Sciences
Hiroaki Matsui, Hisashi Harada, Kanako Maeda, Toshiki Sugiyama, Yohei Fukuchi, Nanae Kimura, Hermanus Nawaly, Yoshinori Tsuji, Yusuke Matsuda
Summary: Marine diatoms express genes encoding potential phosphate transporter and alkaline phosphatase under phosphate-limited condition. The study reveals the importance of phosphate transporters in the initial acclimation stage to phosphate-limited condition and the increasing role of alkaline phosphatase in prolonged phosphate-starved condition.
Article
Chemistry, Analytical
Hongchen Li, Ning Huang, Jing Cheng, Min Ouyang, Jingbo Sun, Chenzhan Peng, Xuan Cao, Dong Xu
Summary: A novel fluorometric method for determining ALP activity using RME as a substrate was proposed to verify the pasteurization of milk products. The method is simple, cost-effective, and shows a good linear relationship between the fluorescence intensity and ALP activity under optimal conditions.
MICROCHEMICAL JOURNAL
(2023)
Article
Oceanography
Xia Zhang, Jingping Zhang, Huamao Yuan, Yuan Shen, Changhao Zhou, Xiaoping Huang
Summary: The study presented the seasonal distributions of alkaline phosphatase activity (APA) in Jiaozhou Bay, North China, which showed variations in different seasons along with phosphorus concentrations, planktonic biomass, and other environmental factors. The findings suggested relief from phosphate limitation for phytoplankton and bacteria in winter, while persistent phosphate stress in other three seasons. Additionally, it was hypothesized that plankton might compensate for phosphate deficiency by promoting enzyme production and improving enzyme affinity.
CONTINENTAL SHELF RESEARCH
(2021)
Review
Endocrinology & Metabolism
Jose A. Riancho
Summary: Increased serum levels of alkaline phosphatase (ALP) are recognized as a marker for liver or bone disorders, while the approach for patients with low ALP is not well-established. Low serum ALP can be caused by severe injuries or diseases, drug therapy, malnutrition, vitamin and mineral deficiencies, and endocrine disorders. Hypophosphatasia, caused by pathogenic variants of the ALPL gene, is the most common genetic cause of low ALP. Severe cases in children often show marked bone hypomineralization, while adult forms typically present with milder symptoms.
CALCIFIED TISSUE INTERNATIONAL
(2022)
Article
Cell Biology
Cara-Lesley Bartlett, Eleanor Margaret Cave, Nigel John Crowther, William Frank Ferris
Summary: Tissue-nonspecific alkaline phosphatase (TNAP) is an isozyme with various isoforms. Its function in many tissues is largely unknown, except for the bone isoform which is well characterized in mineralization. Recent studies have shown that TNAP is expressed in preadipocytes and may play a role in lipid accumulation, although the exact mechanism is unclear.
MOLECULAR AND CELLULAR BIOCHEMISTRY
(2022)
Review
Chemistry, Inorganic & Nuclear
Yudong Wu, Wujun Chen, Chao Wang, Dongming Xing
Summary: Alkaline phosphatase (ALP) is a zinc-containing metalloprotein widely distributed in mammalian tissues that plays a vital role in clinical diagnosis. It is widely used for signal amplification in enzyme-linked assays and is a promising biomarker for disease diagnosis. Assays using L-ascorbic acid 2-phosphate (AAP) as a substrate have been developed to detect ALP activity, providing new insights into the construction of advanced sensing platforms for clinical diagnosis.
COORDINATION CHEMISTRY REVIEWS
(2023)
Article
Chemistry, Analytical
Dong-En Wang, Shangqi You, Wenjing Huo, Xiang Han, Huiyun Xu
Summary: A colorimetric assay based on polydiacetylenes nano-liposomes was developed for the detection of alkaline phosphatase (ALP) activity. The method utilized the interaction between pyridoxal phosphate and the nano-liposomes, causing distinct color changes that were correlated with ALP level. The proposed approach showed good sensitivity and accuracy, and was successfully applied to real human serum samples.
Article
Chemistry, Analytical
Haiyan Li, Shangqing Zhang, Mingli Chen, Jianhua Wang
Summary: Bimetallic dual-ligands terbium nanocomplexes with unique internal structure were fabricated through in situ coordination assembly. By regulating the proportion of internal ligand SSA, the nanocomplexes showed excellent sensing specificity for detecting phosphate and pyrophosphate, with low detection limits.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Genetics & Heredity
Tobias Schmidt, Constantin Schmidt, Michael Amling, Jan Kramer, Florian Barvencik
Summary: This study estimates the prevalence of low AP activity and elevated PLP levels in a population in northern Germany, suggesting that 0.52% of subjects show laboratory signs of HPP. The data support the notion that the prevalence of moderate forms of HPP may be higher than expected.
ORPHANET JOURNAL OF RARE DISEASES
(2021)
Article
Chemistry, Analytical
Liu Huang, Xiaozheng Cao, Tang Gao, Bin Feng, Xueyan Huang, Rong Song, Tao Du, Shijun Wen, Xueping Feng, Wenbin Zeng
Summary: A fluorescent probe THP consisting of AIE and ESIPT was developed for highly selective and sensitive detection of ALP activity, showing potential for biomedical applications in monitoring ALP activity in living cells.
Article
Limnology
Lingling Wan, Chunlei Song, Yiyong Zhou, Xiuyun Cao
Summary: In this study, we review the current knowledge of extracellular phosphatase excreted by cyanobacteria, highlighting the development of detection method and its ecological roles in regulating phosphorus cycling in freshwater systems. Recommendations for the study of extracellular phosphatase produced by bloom-forming cyanobacteria are suggested, followed by a discussion of future prospects.
JOURNAL OF OCEANOLOGY AND LIMNOLOGY
(2022)
Article
Soil Science
Yue Yuan, Shuang Gai, Chunyu Tang, Yongxu Jin, Kui Cheng, Markus Antonietti, Fan Yang
Summary: This study investigates the effects of artificial humic substances (A-HS) on maize agronomic traits and soil phosphorus transformation. The results show that A-HS has positive effects on maize growth and phosphorus uptake. Furthermore, A-HS influences the structure of soil microbial communities and enhances soil phosphorus effectiveness and nutrient uptake by maize.
APPLIED SOIL ECOLOGY
(2022)
Article
Plant Sciences
Jiajia Luo, Zhijian Chen, Rui Huang, Yuanhang Wu, Chun Liu, Zeping Cai, Rongshu Dong, Jacobo Arango, Idupulapati Madhusudana Rao, Rainer Schultze-Kraft, Guodao Liu, Pandao Liu
Summary: In this study, transcriptomic and proteomic analyses identified Pi starvation-inducible APase genes in stylo. Among them, SgPAP7 was found to be a key gene that plays a major role in stylo adaptation to low-Pi conditions by facilitating the utilization of both exogenous and endogenous organic phosphorus sources.
Article
Engineering, Environmental
Jing Zhou, Ruijie Fu, Haoran Liu, Yanlin Liu, Yiwen Wang, Bining Jiao, Yue He, Hongwu Tang
Summary: The study introduces a high-resolution colorimetric biosensor based on multiple hybridization chain reactions (HCRs) and gold nanoparticles (AuNP) for ultrasensitive detection of the Staphylococcus aureus (S. aureus) mecA gene. The method demonstrates high specificity and sensitivity, capable of detecting target DNA concentrations as low as 2.71 pM with rainbow-like colors associated with different DNA concentrations. This approach has potential applications in clinical diagnosis.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Marine & Freshwater Biology
Chao Zhang, Senjie Lin
Article
Agriculture, Multidisciplinary
Yong Xia, Taek Won Kang, Young Do Jung, Chao Zhang, Sen Lian
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2019)
Review
Medicine, Research & Experimental
Chao Zhang, Si Tong Huo, Zhiyonf Wu, Lina Chen, Chang Wen, Honghao Chen, William W. Du, Nan Wu, Daogang Guan, Sen Lian, Burton B. Yang
MOLECULAR THERAPY-NUCLEIC ACIDS
(2020)
Review
Medicine, Research & Experimental
Chao Zhang, RongFang Ding, YiCheng Sun, Si Tong Huo, Alina He, Chang Wen, HongHao Chen, William W. Du, WeiNan Lai, Huijun Wang
Summary: Circular RNAs regulate gene expression through various mechanisms and play important roles in tumorigenesis and metastasis in cancer.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2021)
Review
Biochemistry & Molecular Biology
Yining Gao, Jiawen Gao, Fei Lin, Ting Wang, Sitong Huo, Jiefang Wu, Qi Zhou, Chao Zhang
Summary: Circular RNAs (circRNAs) are endogenous, non-coding RNAs that can play a role in the progression of various diseases. They mainly act as RNA sponges and have been found to regulate transcription and translation, host genes, and the radiosensitivity of tumors.
Article
Toxicology
Youqin Xu, Lina Chen, Mengyi Liu, Yanfang Lu, Yanwei Yue, Yue Liu, Honghao Chen, Fuliang Xie, Chao Zhang
TOXICOLOGY RESEARCH
(2019)