Prostaglandin E2 promotes BeWo spheroids implantation in RL95-2 cell monolayers

Purpose: Integrin v3 (ITG v3) participates in the process of implantation between the embryo and the endometrium. This study investigated the effects of prostaglandin E-2 (PGE(2)) on endometrial receptivity and implantation efficiency of the embryo, and their possible mechanisms.Methods: Quantitative real-time reverse transcription PCR (qRT-PCR) and western blotting were used to detect the changes in mRNA and protein levels of ITG v3 in RL95-2 cells after administering PGE(2). BeWo trophoblast cells and RL95-2 endometrial epithelial cells were used to establish an in vitro model, which was used to observe the adhesion rate and spreading efficiency between BeWo spheroids and RL95-2 cell monolayers after pretreatment with different concentrations of PGE(2).Results: PGE(2) at 200nM increased the mRNA and protein levels of ITG v significantly (p<0.05); 100nM PGE(2) increased the mRNA and protein levels of ITG 3 significantly (p<0.05). PGE(2) at 200nM increased significantly the adhesion and spreading efficiency of BeWo spheres to RL95-2 cell monolayers.Conclusions: An appropriate concentration of PGE(2) might increase the expression of ITG v3, which would, promote embryo adhesion and spreading efficiency. This study provides further evidence that increased expression of ITG v3 might promote implantation by improving endometrial receptivity.