Journal
GENOMICS
Volume 109, Issue 1, Pages 16-26Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ygeno.2016.11.008
Keywords
Human induced pluripotent stem cells; Hepatocytes; Liver; Bioreactor
Funding
- LIMMS internal grant
- PEPS CNRS/Universite de Lille (3D-muHepato-Epi-iPS)
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We have compared the transcriptomic profiles of human induced pluripotent stem cells after their differentiation in hepatocytes like cells in plates and microfluidic biochips. The biochips provided a 3D and dynamic support during the cell differentiation when compared to the 2D static cultures in plates. The microarray have demonstrated the up regulation of important pathway related to liver development and maturation during the culture in biochips. Furthermore, the results of the transcriptomic profile, coupled with immunostaining, and RTqPCR analysis have shown typical biomarkers illustrating the presence of responders of biliary like cells, hepatocytes like cells, and endothelial like cells. However, the overall tissue still presented characteristic of immature and foetal patterns. Nevertheless, the biochip culture provided a specific micro-environment in which a complex multicellular differentiation toward liver could be oriented. (C) 2016 Elsevier Inc. All rights reserved.
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