Journal
GENETICS
Volume 207, Issue 3, Pages 949-959Publisher
GENETICS SOCIETY AMERICA
DOI: 10.1534/genetics.117.300317
Keywords
telomere; terminin; Drosophila; double strand break; hiphop; HOAP; FLP; dicentric chromosome
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Funding
- National Institute of General Medical Sciences of the NIH [RO1GM065604]
- Southeast Missouri State University
- Epigenomics Flagship Project EpiGen
- Italian Ministry of Education and Research, National Research Council
- Intramural Program of the National Cancer Institute
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The addition of a new telomere onto a chromosome break, a process termed healing, has been studied extensively in organisms that utilize telomerase to maintain their telomeres. In comparison, relatively little is known about how new telomeres are constructed on broken chromosomes in organisms that do not use telomerase. Chromosome healing was studied in somatic and germline cells of Drosophila melanogaster, a nontelomerase species. We observed, for the first time, that broken chromosomes can be healed in somatic cells. In addition, overexpression of the telomere cap component Hiphop increased the survival of somatic cells with broken chromosomes, while the cap component HP1 did not, and overexpression of the cap protein HOAP decreased their survival. In the male germline, Hiphop overexpression greatly increased the transmission of healed chromosomes. These results indicate that Hiphop can stimulate healing of a chromosome break. We suggest that this reflects a unique function of Hiphop: it is capable of seeding formation of a new telomeric cap on a chromosome end that lacks a telomere.
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