Article
Biochemistry & Molecular Biology
Xiang Xiao, Yurong Zhou, Cui Tan, Juan Bai, Ying Zhu, Jiayan Zhang, Xinghua Zhou, Yansheng Zhao
Summary: Barley beta-glucan shows potential in defending oxidative stress in C. elegans by reducing ROS levels, enhancing SOD and CAT enzyme activity, decreasing MDA content, and extending lifespan. It participates in anti-oxidative stress partially through the daf-2/daf-16 pathway.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Engineering, Marine
Peiqi Sun, Xuwen Cao, Liusuo Zhang
Summary: This study investigates the effects of different diets on the development and lifespan of nematodes. The results show that the CeMM diet attenuates the development but not the lifespan of marine nematodes. In C. elegans, the CeMM diet significantly increases the expression of genes related to FOXO DAF-16 signaling and lysosome and xenobiotic metabolism, which may contribute to the extension of lifespan.
JOURNAL OF MARINE SCIENCE AND ENGINEERING
(2022)
Article
Immunology
Yi Xiao, Li Zhang, Xinting Zhu, Ying Qin, Changyan Yu, Nian Jiang, Sanhua Li, Fang Liu, Yun Liu
Summary: The DAF-2/DAF-16 insulin-like signaling pathway regulates various aspects of organismal physiology and is conserved throughout evolution. Luteolin, a flavone found in many plants and vegetables, has been shown to have anti-tumor, antioxidant, and neuroprotective effects. This study found that luteolin increased resistance to both Gram-negative and Gram-positive pathogens in a dose-dependent manner and promoted host immune response. Furthermore, it was discovered that luteolin activated the DAF-16/FOXO transcription factor and promoted innate immunity through the DAF-2/DAF-16 insulin-like signaling pathway.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Cell Biology
Huihui Ji, Zhimin Qi, Daniel Schrapel, Monika Le, Yiqiao Luo, Bin Yan, Jury Gladkich, Michael Schaefer, Li Liu, Ingrid Herr
Summary: Sulforaphane can prolong lifespan and healthspan by inducing the expression and nuclear translocation of TRA-1/GLI, and thus support healthy aging by regulating daf-16/FOXO. However, the prolonging effects of sulforaphane on lifespan and healthspan are inhibited when TRA-1 or daf-16 is interfered with by RNA interference or using worms with mutants of tra-1 or daf-16 genes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Medicine, Research & Experimental
Xingzhuo Song, Yu Du, Cen Liu, Wei Wang, Jing Han, Xinlou Chai, Yonggang Liu
Summary: Oxidative stress disrupts redox state homeostasis and induces apoptosis. Prolonged oxidative stress can impair cell, tissue, and organ function, leading to the development of diseases. H-2 synthesized from N-Alkylamides (NAAs) of Anacyclus pyrethrum (L.) DC was investigated for its antioxidant activity and molecular mechanisms using Caenorhabditis elegans (C. elegans) and B16-F10 mouse melanoma cell models. In vivo studies on C57BL/6 mice showed the anti-vitiligo activity of H-2. The results demonstrated that H-2 increased the survival time of nematodes under oxidative stress, promoted the nuclear localization of DAF-16, and enhanced the expression of Superoxide Dismutase 3 (SOD-3) in nematodes, thus activating the antioxidant enzyme system. H-2 also reversed oxidative stress damage in the B16-F10 cell model and increased the number of melanocytes in vitiligo model mice, suggesting its potential in vitiligo treatment.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Biochemistry & Molecular Biology
Zong-Ping Zhang, Xue Bai, Wen-Bo Cui, Xiao-Han Chen, Xu Liu, De-Juan Zhi, Zhan-Xin Zhang, Dong-Qing Fei, Dong-Sheng Wang
Summary: This study found that Caesalmin C can delay the progression of AD in the transgenic C. elegans model by inhibiting A beta deposition, upregulating the expression of genes associated with oxidative stress, inhibiting acetylcholinesterase activity, and upregulating proteasome activity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Zhimin Qi, Huihui Ji, Monika Le, Hanmei Li, Angela Wieland, Sonja Bauer, Li Liu, Michael Wink, Ingrid Herr
Summary: Sulforaphane extends the lifespan of C. elegans by modulating the insulin/IGF-1 signaling pathway, promoting healthspan by increasing mobility and appetite while reducing lipofuscin accumulation.
Article
Biochemistry & Molecular Biology
Jiahao Zhu, Yumei Jia, Cong Wang, Wangting Zhou, Yifan Shu, Kaixin Zhang, Xiaoxiong Zeng, Renpeng Guo
Summary: This study investigated the anti-aging activities of Lonicera japonica polysaccharides (LJPs) and revealed their mechanism of action. LJPs were found to prolong the lifespan of Caenorhabditis elegans, improve its fitness, reduce lipofuscin accumulation, and activate stress-related genes and antioxidant system. These effects were mediated by the DAF-16 pathway.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Fangru Wang, Ting Jin, Hongyuan Li, Houfang Long, Ying Liu, Sha Jin, Yuyuan Lu, Yinghua Peng, Cong Liu, Lihui Zhao, Xiaohui Wang
Summary: Cannabidivarin (CBDV), a structural analog of cannabidiol (CBD), inhibits the aggregation of alpha-synuclein (alpha-syn) and protects DAergic neurons from oxidative stress and degeneration in a Caenorhabditis elegans model. The phenolic hydroxyl groups of CBDV are essential for its antioxidant activity and the prevention of alpha-syn aggregation.
Article
Biochemistry & Molecular Biology
Enrico Castroflorio, Joery den Hoed, Daria Svistunova, Mattea J. Finelli, Alberto Cebrian-Serrano, Silvia Corrochano, Andrew R. Bassett, Benjamin Davies, Peter L. Oliver
Summary: Members of the Tre2/Bub2/Cdc16 (TBC), lysin motif (LysM), domain catalytic (TLDc) protein family are associated with neurodevelopmental disorders, including NCOA7 linked to autism. NCOA7 is shown to regulate the function of the vacuolar (V)-ATPase in the brain, with its deletion resulting in neuronal developmental defects and lysosomal dysfunction. Behavioral assessment of Ncoa7 deletion mice revealed anxiety and social defects, indicating the importance of NCOA7 in neurodevelopment.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Geriatrics & Gerontology
Shuju Bai, Wenbo Wang, Zhiwei Zhang, Mengyao Li, Zehan Chen, Jiuqiao Wang, Yanlin Zhao, Lu An, Yuxiang Wang, Shu Xing, Xueqi Fu, Junfeng Ma
Summary: Ethanol can attenuate amyloid-beta toxicity in the Alzheimer's disease model by promoting amyloid-beta degradation and reducing amyloid-beta aggregation.
FRONTIERS IN AGING NEUROSCIENCE
(2021)
Article
Environmental Sciences
Chi-Wei Huang, Wan-Ru Liao, Chun Ming How, Pei-Ling Yen, Chia-Cheng Wei
Summary: The study found that ZEN significantly decreased locomotive behaviors in C. elegans. Lifespan and aging markers were also adversely affected by ZEN, which increased ROS levels and downregulated antioxidant genes. Long-term exposure to ZEN induced aging-related decline in C. elegans, associated with oxidative stress and inhibition of antioxidant defense.
ENVIRONMENTAL POLLUTION
(2021)
Article
Pharmacology & Pharmacy
Zhenghan Dong, Yachao Wang, Cuiting Hao, Yuan Cheng, Xi Guo, Yanyu He, Yueyue Shi, Shuang Wang, Yunqi Li, Wei Shi
Summary: This study found that the extract of S. Sanghuang (SSE) has positive effects on nematode indicators. Different concentrations of SSE can prolong the lifespan of nematodes and increase it by 26.41%. SSE also reduces the accumulation of endogenous aging substances, increases antioxidant capacity and enhances physical fitness. RT-PCR analysis shows that SSE treatment upregulates the expression of a series of genes, prolonging the lifespan of nematodes. This study reveals the new role of S. Sanghuang in promoting longevity and inhibiting stress, providing a theoretical basis for its application in anti-aging treatments.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Abdel Fawaz Bagoudou, Yifeng Zheng, Masahiro Nakabayashi, Saroat Rawdkuen, Hyun-Young Park, Dhiraj A. Vattem, Kenji Sato, Soichiro Nakamura, Shigeru Katayama
Summary: The study identified the neuroprotective activity of Glochidion littorale leaf extract, which showed high bioactivities in ameliorating oxidative stress and protecting against neurodegeneration in Caenorhabditis elegans. The observed effects may be associated with the activation of the transcription factor DAF-16, and potential key phenolic compounds identified in the extract include myricetin, coumestrin, chlorogenic acid, and hesperidin. This novel neuroprotective activity of GLE may be utilized in developing treatments for neurodegenerative diseases like Parkinson's syndrome.
Article
Food Science & Technology
Jiaqian Hong, Yiming Song, Jiayan Xie, Jianhua Xie, Yi Chen, Ping Li, Danyang Liu, Xiaobo Hu, Qiang Yu
Summary: This study used Caenorhabditis elegans as a model organism to investigate the toxic effects and mechanisms of acrolein (ACR) exposure. The results showed that ACR exposure shortened the lifespan of C. elegans, reduced reproductive capacity and locomotive behavior, and increased oxidative stress levels. ACR also affected the expression of antioxidant-related genes. The findings suggest that ACR exposure induces aging and oxidative stress in C. elegans, activating the DAF-16/FOXO pathway.
Article
Genetics & Heredity
Ata Ghavidel, Kunal Baxi, Vladimir Ignatchenko, Martin Prusinkiewicz, Terra G. Arnason, Thomas Kislinger, Carlos E. Carvalho, Troy A. A. Harkness
Article
Genetics & Heredity
Kunal Baxi, Ata Ghavidel, Brandon Waddell, Troy A. Harkness, Carlos E. de Carvalho
Article
Genetics & Heredity
Ata Ghavidel, Kunal Baxi, Martin Prusinkiewicz, Cynthia Swan, Zach R. Belak, Christopher H. Eskiw, Carlos E. Carvalho, Troy A. Harkness
G3-GENES GENOMES GENETICS
(2018)
Article
Multidisciplinary Sciences
Kunal Baxi, Carlos E. de Carvalho
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2018)
Article
Biology
Myron S. Ignatius, Madeline N. Hayes, Finola E. Moore, Qin Tang, Sara P. Garcia, Patrick R. Blackburn, Kunal Baxi, Long Wang, Alexander Jin, Ashwin Ramakrishnan, Sophia Reeder, Yidong Chen, Gunnlaugur Petur Nielsen, Eleanor Y. Chen, Robert P. Hasserjian, Franck Tirode, Stephen C. Ekker, David M. Langenau
Article
Multidisciplinary Sciences
Silvia Pomella, Prethish Sreenivas, Berkley E. Gryder, Long Wang, David Milewski, Matteo Cassandri, Kunal Baxi, Nicole R. Hensch, Elena Carcarino, Young Song, Hsien-Chao Chou, Marielle E. Yohe, Benjamin Z. Stanton, Bruno Amadio, Ignazio Caruana, Cristiano De Stefanis, Rita De Vito, Franco Locatelli, Yidong Chen, Eleanor Y. Chen, Peter Houghton, Javed Khan, Rossella Rota, Myron S. Ignatius
Summary: SNAI2 is highly expressed in FN-RMS, activated by MYOD through super enhancers, competes with MYOD at certain myogenic enhancers, and blocks differentiation.
NATURE COMMUNICATIONS
(2021)
Article
Developmental Biology
Amanda Lipsitt, Nicole R. Hensch, Rodrigo Moreno-Campos, Kunal Baxi, Jiangfei Chen, Anil K. Challa, Eleanor Y. Chen, Myron S. Ignatius
Summary: Angiosarcoma, an aggressive tumor with high mortality rate, can spontaneously develop in zebrafish mutant models. A new tumor grafting technique has been developed to address the low engraftment rates of traditional protocols, offering superior engraftment rates while maintaining the tumor microenvironment.