Journal
GENESIS
Volume 55, Issue 10, Pages -Publisher
WILEY
DOI: 10.1002/dvg.23067
Keywords
DIO; Dppa3; DTA; En1; loxP; Rosa26
Categories
Funding
- US National Institutes of Health, National Institute of Environmental Health Sciences [ES102805, ES102965]
Ask authors/readers for more resources
Recombinase responsive mouse lines expressing diphtheria toxin subunit A (DTA) are well established tools for targeted ablation of genetically defined cell populations. Here we describe a new knock-in allele at the Gt(Rosa)26Sor locus that retains the best features of previously described DTA allelesincluding a CAG promoter, attenuated mutant DTA cDNA, and ubiquitous EGFP labeling with the addition of a Cre-dependent FLEx switch for tight control of expression. The FLEx switch consists of two pairs of antiparallel lox sites requiring Cre-mediated recombination for inversion of the DTA to the proper orientation for transcription. We demonstrate its utility by Cre-dependent ablation of both a broad domain in the embryonic nervous system and a discrete population of cells in the fetal gonads. We conclude that this new DTA line is useful for targeted ablation of genetically-defined cell populations.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available