Journal
GENE
Volume 636, Issue -, Pages 23-29Publisher
ELSEVIER
DOI: 10.1016/j.gene.2017.08.036
Keywords
Fat deposition; Pork quality; PPARG; miR-130a
Categories
Funding
- National Key Technology Research and Development Program of the Ministry of Science and Technology [2015BAD03B01]
- Natural Science Foundation of Jiangsu Province [BK20160727]
- National Natural Science Foundation of China [31272423]
Ask authors/readers for more resources
To investigate the molecular mechanism underlying differential lipid deposition between intramuscular (IM) and subcutaneous (SC) fat tissues of pigs, peroxisome proliferator-activated receptor gamma (PPARG) expression levels were compared in these two types of adipose tissues. The results showed that both mRNA and protein levels of PPARG were significantly higher in SC fat than in IM fat. Correspondingly, the expression levels of miR-128 and miR-130a, which potentially targeting PPARG 3'-untranslated region (3'-UTR), in IM fat were 8.37 and 2.30-fold of those in SC fat respectively. Further dual luciferase activity assay showed that only miR-130a directly targeting PPARG. Moreover, over-expression of miR-130a in preadipocytes significantly inhibited its differentiation by suppressing PPARG expression. In conclusion, tissue variance of miR-130a levels results in the diverse of PPARG, and might be the reason for differential fat deposition between IM and SC fat tissues in pigs. Our study would provide the molecular foundation for IM fat deposition increase and therefore contribute to pork quality improvement.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available