Journal
FUTURE MICROBIOLOGY
Volume 12, Issue 5, Pages 379-392Publisher
FUTURE MEDICINE LTD
DOI: 10.2217/fmb-2016-0127
Keywords
16S rRNA; biofilm; exacerbation; lung microbiome; P. aeruginosa; severe COPD
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Funding
- Fundacio Catalana de Neumologia
- Sociedad Catalana de Neumologia
- Sociedad Espanola de Neumologia y Cirugia Toracica
- CIBER de Enfermedades Respiratorias (CIBERES)
- CERCA Programme/Generalitat de Catalunya
- ISCIII-General Evaluation Branch
- European Regional Development Fund (ERDF)
- [PI09/1904]
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Aim: The bronchial microbiome of severe chronic obstructive pulmonary disease patients colonized by Pseudomonas aeruginosa was analyzed using 16S rRNA gene sequencing to identify differences related to biofilm-forming capacity. Patients & methods: Patient sputum samples from 21 patients were studied. Results: Statistically significant differences related to biofilm-forming capacity were only found for genera with relative abundances < 1%, and Fusobacterium was over-represented when biofilm-forming capacity was high. Genera with relative abundances > 50% which increased from baseline were observed in 10/14 exacerbations, but corresponded to Pseudomonas only in three episodes, while other pathogenic genera were identified in seven. Conclusion: The bronchial microbiome shows differences according with P. aeruginosa biofilm-forming capacity. Pathogenic microorganisms other than P. aeruginosa cause a significant part of the exacerbations in colonized chronic obstructive pulmonary disease patients.
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