Journal
FOOD CONTROL
Volume 80, Issue -, Pages 290-296Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodcont.2017.05.016
Keywords
Cyperus rotundus; Essential oil; Antibacterial activity; Membrane disruption; Apoptosis pathway; Staphylococcus aureus
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Funding
- Natural Science Foundation of Shanxi Province, China [201601D011070]
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The chemical composition, antibacterial activity and mechanism of essential oil from Cyperus rotundus rhizomes against Staphylococcus aureus were investigated in this study. Results showed that alpha-cyperone, cyperene and alpha-selinene were the major components of the essential oil. The essential oil exhibited strong antibacterial activity against S. aureus with the minimum inhibitory concentration (MIC) and minimum bactericide concentration (MBC) were 10 and 20 mg/rnL respectively, and the antibacterial effects increased with increasing essential oil concentrations and treatment time. The electric conductivity, cell membrane integrity, NPN uptake, and membrane potential assays demonstrated that essential oil disrupted the membrane integrity of S. aureus. Electron microscope observations further confirmed that essential oil destroyed cell membrane. Moreover, we found that essential oil could induce cells death of S. aureus through apoptosis pathway based on apoptosis analysis. These findings suggested that essential oil mainly exerted antibacterial activity by damaging cell membrane and membrane-mediated apoptosis pathway. (C) 2017 Elsevier Ltd. All rights reserved.
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