Journal
FOOD AND CHEMICAL TOXICOLOGY
Volume 105, Issue -, Pages 285-299Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fct.2017.04.020
Keywords
Fescue toxicosis; Epichloe coenophiala; Ergot alkaloids; Grazing beef cattle; Untargeted metabolomics
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Funding
- USDA, National Institute of Food and Agriculture [67030-25004]
- Interdisciplinary Toxicology Program
- Department of Physiology and Pharmacology in the College of Veterinary Medicine
- Graduate School of the University of Georgia
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Fescue toxicosis (FT) results from consumption of tall fescue (Lolium arundinaceum) infected with an endophyte (Epichloe coenophiala) that produces ergot alkaloids (EA), which are considered key etiological agents of FT. Decreased weight gains, hormonal imbalance, circulating cholesterol disruption, and decreased volatile fatty acid absorption suggest toxic (E+) fescue-induced metabolic perturbations. Employing untargeted high-resolution metabolomics (HRM) to analyze E+ grazing-induced plasma and urine metabolome changes, fescue-naive Angus steers were placed on E+ or non-toxic (Max-Q) fescue pastures and plasma and urine were sampled before, 1, 2, 14, and 28 days after pasture assignment. Plasma and urine catecholamines and urinary EA concentrations were also measured. In E+ steers, urinary EA appeared early and peaked at 14 days. 13,090 urinary and 20,908 plasma HRM features were detected; the most significant effects were observed earlier (2 days) in the urine and later (>= 14 days) in the plasma. Alongside EA metabolite detection, tryptophan and lipid metabolism disruption were among the main consequences of E+ consumption. The E+ grazing-associated metabolic pathways and signatures described herein may accelerate development of novel early FT detection and treatment strategies. (C) 2017 Elsevier Ltd. All rights reserved.
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