Article
Food Science & Technology
Zixin Zhu, Qiuyun Shi, Jianwei Wu, Kangli He, Jianguo Feng, Sa Dong
Summary: Acetamiprid (ACE) is a widely used chemical in vegetables to control pests, but its residues pose a threat to human health. To rapidly detect ACE residues in vegetables, researchers have developed an indirect competitive chemiluminescence enzyme immunoassay (ic-CLEIA), which is simple, sensitive, and accurate.
Article
Chemistry, Analytical
Xinxin Xu, Lingling Guo, Hua Kuang, Liguang Xu, Chuanlai Xu, Liqiang Liu
Summary: In this study, novel haptens were designed and a broad-specific monoclonal antibody was developed for the rapid detection of anthranilic diamide insecticides in vegetables and fruits, demonstrating the convenience and practicality of this method.
Article
Electrochemistry
Inga Morkvenaite-Vilkonciene, Aura Kisieliute, Wojciech Nogala, Anton Popov, Benediktas Brasiunas, Mindaugas Kamarauskas, Arunas Ramanavicius, Steven Linfield, Almira Ramanaviciene
Summary: In this research, an electrochemical sandwich immunoassay using scanning electrochemical microscopy on a gold micro-disc array was proposed for the detection of human growth hormone. It was shown that hGH concentrations as low as 40 nM could be detected using this method.
ELECTROCHIMICA ACTA
(2023)
Article
Chemistry, Applied
Peng Dai, Yan Zhang, Yanping Hong, Jianhua Xiong, Huaying Du, Luying Duan, Dan Wang, Yubo Wang, Weijie Deng, Bruce D. Hammock, Wuying Yang
Summary: A sensitive and reliable icCLEIA method was developed to detect gentamicin residues in animal tissues. By preparing an anti-gentamicin monoclonal antibody and utilizing immunological analysis techniques, gentamicin can be detected rapidly and accurately.
Article
Chemistry, Analytical
Lu Lin, Shanshan Song, Xiaoling Wu, Liqiang Liu, Hua Kuang, Jing Xiao, Chuanlai Xu
Summary: This study developed an ic-ELISA and immunochromatographic strip assay for rapid screening of robenidine hydrochloride (ROBH), showing high specificity and good recovery rates. Both methods yielded results consistent with LC-MS/MS analysis, making them effective for detecting ROBH in shrimp, chicken breast, and chicken liver samples.
Article
Chemistry, Analytical
Dmitriy V. Sotnikov, Anatoly V. Zherdev, Boris B. Dzantiev
Summary: The detection of disease-specific antibodies in blood (serodiagnosis) is an effective method in medical analytical chemistry. Serodiagnostics in lateral flow immunoassay format meet modern requirements for rapid testing, but the presence of nonspecific immunoglobulins in samples poses limitations that need to be addressed.
Article
Chemistry, Analytical
Xinfa Cai, Meijuan Liang, Fei Ma, Sherif Ramzy Mohamed, Amira Abdel Goda, Dawood H. Dawood, Li Yu, Peiwu Li
Summary: A direct competitive nanozyme-linked immunosorbent assay based on MnO2 nanosheets as a nanozyme label was developed for highly sensitive determination of fumonisin B-1. The method showed a wide linear range, low detection limit, high accuracy, and good consistency with the reference method for screening FB1 in cereals.
ANALYTICAL METHODS
(2021)
Article
Biochemical Research Methods
Salome Yakubu, Boyuan Jia, Yujia Guo, Yanmin Zou, Ninghui Song, Jianxuan Xiao, Kunlong Liang, Yuanqing Bu, Zhen Zhang
Summary: An indirect competitive immunosensor has been developed for TBBPA detection based on a signal amplification system. The Pd nanospheres used as the label for the secondary antibody and the MWCNTs/Au-TB platform for the immunosensor contributed to the improved sensitivity and stability of the sensor. Furthermore, the strategy showed promising results for analyzing emerging pollutants in real water samples with acceptable accuracy and recoveries.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Food Science & Technology
Ying Ying, Xueyan Cui, Hui Li, Lingyi Pan, Ting Luo, Zhen Cao, Jing Wang
Summary: A direct competitive ELISA and a magnetic lateral flow immunoassay (MLFIA) were developed to detect residues of Halosulfuron-methyl (HM) in tomato and maize matrices and paddy water, respectively. These methods showed high sensitivity and accuracy in the rapid detection of HM residues.
Article
Agriculture, Multidisciplinary
Ziang Zhang, Hong Lin, Jianxin Sui, Xiangning Han, Luefeng Wang, Xun Sun, Limin Cao
Summary: Chlorophyll was identified as a significant source of matrix interference in the immunoassay of procymidone in vegetables, and a new pretreatment method using 5-sulfosalicylic acid was developed to eliminate chlorophyll and improve the analysis efficiency.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2022)
Article
Chemistry, Analytical
Meng Dang, Zizhe Li, Yexuan Mao, Xianqing Huang, Lianjun Song, Wenfeng Li, Ruxiang Ma, Yang Liu, Liye Wang, Xuezhi Yu, Huijuan Yang, Xiya Zhang
Summary: A new highly sensitive lateral flow immunoassay (LFA) based on amorphous carbon nanoparticles (ACNs) was developed for high-throughput screening of sulfonamides (SAs) residues in milk samples, especially sulfamethazine (SM2). This LFA utilized a group-specific monoclonal antibody 10H7 (mAb 10H7) conjugated to ACNs as an immune probe, achieving a sensitive detection with a cut-off value of 2 ng/mL for 25 SAs. The LFA was successfully applied for screening SAs residues in real milk samples, showing consistent results with HPLC-MS/MS.
Article
Entomology
Sanower Warsi, Ana M. Chicas-Mosier, Rammohan R. Balusu, Alana L. Jacobson, Henry Y. Fadamiro
Summary: This study examined the interaction between two egg parasitoids, Paratelenomus saccharalis and Ooencyrtus nezarae. The results showed that simultaneous release of both parasitoids led to higher total host egg parasitism compared to sequential release. P. saccharalis displayed aggressive competitive behavior, while O. nezarae larvae outcompeted in multiparasitized eggs. These findings have important implications for the success of biological control programs targeting the kudzu bug.
Article
Food Science & Technology
Zile Wang, Liang Zhang, YanHong Yang, Huixia Zhang, Weichunbai Zhang, Pimiao Zheng, Haiyang Jiang
Summary: This study aimed to establish a broad-spectrum immunoassay for rapid detection and monitoring of benzimidazoles (BZs) in milk. An ultra-sensitive and broad-spectrum antibody was obtained through hapten modification. The established indirect competitive enzyme-linked immunosorbent assay (icELISA) could simultaneously identify 18 BZs in milk with good accuracy and precision.
FOOD QUALITY AND SAFETY
(2023)
Article
Chemistry, Applied
Chia-Lin Tsai, I-Ning Chen, Yi-Tien Chen
Summary: Mollusks, a major allergenic food, must be declared on food products to reduce the risk of allergies. However, there is currently no reliable immunoassay for detecting edible mollusks. In this study, a sandwich enzyme-linked immunosorbent assay (sELISA) was developed to detect 32 edible mollusk species without cross-reaction with non-mollusk species. The assay proved effective in detecting various cooked and raw mollusk samples, as well as commercial mollusk products.
Article
Chemistry, Multidisciplinary
Abiola Adesina, Philani Mashazi
Summary: This work presents two simple and sensitive label-free impedimetric immunoassays for the detection of C-reactive protein (CRP). The direct immunoassay exhibited a linear curve for a concentration range up to 100 ng ml(-1), with a limit of detection (LoD) of 2.9 ng ml(-1). XPS and electrochemical impedance confirmed the immobilization of anti-CRP mAb. The sandwich immunoassay improved the LoD to 1.2 ng ml(-1) and enhanced the sensitivity to 0.885 k omega ng(-1) ml cm(-2) for the detection of the CRP antigen.
FRONTIERS IN CHEMISTRY
(2021)
Article
Infectious Diseases
Yury A. Surovoy, Maksim A. Burkin, Inna A. Galvidis, Pavel O. Bochkov, Armen V. Oganesyan, Sergei V. Tsarenko
Summary: This study aimed to describe the pharmacokinetics of polymyxin B in patients receiving veno-venous extracorporeal membrane oxygenation (ECMO) compared to critically ill patients without ECMO support. The results showed that the currently recommended dosage of polymyxin B is sufficient for ECMO patients and no dosage increase is required.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2022)
Article
Food Science & Technology
Maksim A. Burkin, Inna A. Galvidis, Sergei A. Eremin
Summary: The study found that the presence of avidin and biotin in food matrices may interfere with the accuracy of (strept)avidin-biotin-based immunoanalytical systems for food safety. Competitive ELISA methods based on hapten-protein coating conjugates and biotinylated analytes bound to immobilized streptavidin showed improved sensitivity in the determination of bacitracin and colistin. These methods also allowed for the assessment of avidin and biotin content in food matrices.
Article
Chemistry, Analytical
Maksim A. Burkin, Anastasia G. Moshcheva, Inna A. Galvidis
Summary: This work focuses on developing an immunoassay to investigate the widespread use of natamycin (NAT) in food preservation. By designing immunogens and optimizing the extraction procedure, a sensitive ELISA variant was developed for the detection of NAT. The screening examination revealed the undeclared use of NAT in yogurt products.
Article
Chemistry, Analytical
Maksim A. Burkin, Yuri A. Surovoy, Vera G. Arzumanian, Inna A. Galvidis
Summary: This study developed a novel immunoassay for ATB in human serum samples, providing crucial support for clinical dosing and monitoring in patients.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2022)
Article
Chemistry, Analytical
Inna A. Galvidis, Yury A. Surovoy, Gleb S. Perevoznyuk, Sergei V. Tsarenko, Maksim A. Burkin
Summary: Polymyxins are used to treat multidrug resistant bacteria, but their narrow therapeutic window and variable pharmacokinetics in critically ill patients pose risks of toxicity and underdosing. This study explored methods for determining unbound polymyxin B (PMB) and proposed limited sampling strategies for clinical application. Rapid equilibrium dialysis demonstrated lower non-specific binding compared to ultrafiltration, making it a more reliable tool. The study also suggested using 1-3 sampling points and Bayesian estimation as an alternative approach.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2022)
Article
Pharmacology & Pharmacy
Yury A. Surovoy, Maksim A. Burkin, Inna A. Galvidis, Mikhail A. Sobolev, Onur Can Rende, Sergei Tsarenko
Summary: This study assesses the pharmacokinetics of polymyxin B in patients with varying degrees of renal dysfunction and in patients requiring continuous veno-venous hemodialysis. The results show that patients with renal insufficiency had higher serum concentrations of polymyxin B, indicating a higher risk of toxicity. For patients on CVVHD, a fixed maintenance dose of 100 mg q12h is recommended.
EUROPEAN JOURNAL OF CLINICAL PHARMACOLOGY
(2023)
Article
Chemistry, Analytical
Inna A. Galvidis, Yury A. Surovoy, Sergei V. Tsarenko, Maksim A. Burkin
Summary: Tigecycline (TGC) is a potent and broad-spectrum antibiotic that can overcome tetracycline resistance. To improve safety and efficacy, an immunodetection method using TGC selective antibodies and group-specific antibodies was developed for therapeutic drug monitoring. The method showed high sensitivity and satisfactory recovery rates in healthy volunteers and COVID-19 patients with secondary bacterial infections. Pharmacokinetic study conducted in critically ill patients supported the suitability of the test for individual therapy monitoring and population pharmacokinetic modeling.
Article
Chemistry, Analytical
Maksim A. Burkin, Anna N. Tevyashova, Elena N. Bychkova, Artem O. Melekhin, Inna A. Galvidis
Summary: Macrolide antibiotics are widely used in medicine, veterinary medicine, and agriculture, resulting in their presence as environmental pollutants worldwide. This study presents an underexploited strategy to enhance the detectability of macrolides in environmental samples by analyzing larger volumes of test samples. The proposed method significantly improved assay sensitivity and detection limit by more than 50-fold.
