Article
Immunology
Sen-Hao Jiang, Lin-Xin Wu, Yu-Ting Cai, Rui-Ting Ma, Hua-Bin Zhang, Dai-Zhen Zhang, Bo-Ping Tang, Qiu-Ning Liu, Li-Shang Dai
Summary: This study analyzed the transcriptomic spectrum of yellow catfish head kidney challenged by Vibrio cholerae using high-throughput sequencing methods. A total of 45,544 unique transcript fragments and 674 differentially expressed genes were identified. Functional analysis showed that these differentially expressed genes were involved in immune response, response to external stimuli, and regulation of immune-related pathways. Real-time quantitative reverse transcription-PCR results confirmed the upregulation of 10 immune response genes after 6 hours of V. cholerae challenge. Overall, this study provides insights into the defense mechanisms and immune system responses of yellow catfish to bacterial challenges.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Fisheries
Senhao Jiang, Yuting Lei, Yanxuan Li, Wanyan Sun, Ti Wang, Ruiting Ma, Qiuning Liu, Boping Tang
Summary: This study investigated the ITLN gene in yellow catfish and quantified its gene expression in response to PAMP stimulation. The results showed that the PfITLN gene was expressed in various tissues, with the highest expression in the liver. After stimulation, the expression levels of PfITLN were upregulated, suggesting its involvement in the innate immune response.
Article
Fisheries
Hua Liu, Jia-fang Xie, Hui Yu, Zheng Ma, Ying-Ying Yu, Ying Yang
Summary: This study sequenced the spleens of E. tarda-infected and non-infected yellow catfish to obtain miRNA and mRNA expression profiles, identifying large numbers of differentially expressed miRNAs and mRNAs and annotating key genes. Through integrated miRNA-mRNA analysis, immune-related miRNA-mRNA pairs were identified, and some differentially expressed miRNAs and mRNAs were verified. Further verification of selected mRNAs using in situ hybridization provided valuable information for understanding the mechanisms of yellow catfish defense against E. tarda infection.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2021)
Article
Physiology
Dexiang Feng, Yangping Yu, Kaifang Liu, Yi Su, Tianyu Fan, Xusheng Guo, Ming Li
Summary: This experiment aimed to investigate the effects of dietary leucine on growth, antioxidant capacity, immune response, and inflammation in juvenile yellow catfish. The results showed that weight gain rate, specific growth rate, and activities of liver superoxide dismutase, glutathione peroxidase, and serum lysozyme, as well as immunoglobulin M content, increased significantly with increasing dietary leucine levels up to 26.00 g kg(-1), but decreased with further increase in dietary leucine. Conversely, the lowest malondialdehyde content was found in the 26.00 and 33.00 g kg(-1) leucine groups. The expression levels of IGF 1 and MYF 5 genes in muscle were upregulated with increasing dietary leucine levels up to 26.00 g kg(-1), while the expression of MSTN level showed the opposite trend. The lowest expression levels of IL 8 and TNF alpha genes in the liver were found in the 26.00 g kg(-1) leucine groups. The quadratic regression analysis indicated that the optimal dietary leucine requirement was estimated to be 26.84-27.00 g kg(-1) of the dry diet.
FRONTIERS IN PHYSIOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Shengtao Guo, Mengsha Zeng, Wenxue Gao, Fan Li, Xiuying Wei, Qiong Shi, Zhengyong Wen, Zhaobin Song
Summary: Yellow catfish is an important fish in China's rivers. Viral and bacterial diseases have caused serious problems in domestic fisheries and threaten aquaculture production. This study investigated the role of TLR3 in the immune response of hybrid yellow catfish to viral infection. The results showed that TLR3 played a significant role and provide a basis for further research and cultivation of disease-resistant yellow catfish.
Review
Virology
Caroline Fernandes-Santos, Elzinandes Leal de Azeredo
Summary: Dengue is a mosquito-borne viral disease caused by the dengue virus, with a wide range of clinical manifestations. In the innate antiviral response, Toll-like receptors (TLRs) play a critical role in pathogen recognition and the production of inflammatory cytokines and interferons essential for preventing viral replication.
Article
Environmental Sciences
Wan-Hong Zeng, Xiu-Ying Wei, Wei Qin, Chuan-Jie Qin, Qiong Shi, Sheng-Tao Guo, Panita Prathomya, Shi-Yong Zhang, Peng Fu, Wei Hu, Han-Wen Yuan, Zheng-Yong Wen
Summary: This study identified and characterized the elovl8 gene from yellow catfish. The gene was highly conserved and played a crucial role in HUFA biosynthesis during early development stages. The expression of elovl8 was inhibited by diets rich in HUFAs.
FRONTIERS IN MARINE SCIENCE
(2023)
Article
Biotechnology & Applied Microbiology
Ying-Yu Tang, Yue-Tian Li, Xiao-Han Zha, Dai-Zhen Zhang, Bo-Ping Tang, Qiu-Ning Liu, Sen-Hao Jiang, Li-Shang Dai
Summary: This study isolated and characterized the PfCFI gene from yellow catfish, showing its differential expression in various tissues and indicating its important role in the fish's immune response to pathogens.
Article
Fisheries
Yu Xia, Cui Liu, Shuzhan Fei, Haokun Liu, Dong Han, Junyan Jin, Yunxia Yang, Xiaoming Zhu, Shouqi Xie
Summary: The experiment showed that dietary supplementation of 1% Arthrospira platensis significantly improved the growth performance and food intake of hybrid yellow catfish, while decreasing the crude lipid content and increasing antioxidant capacity.
AQUACULTURE REPORTS
(2021)
Article
Fisheries
Linyi Bai, Guanliu Yu, Yujie Liu, Muhammad Aizaz, Guiwen Yang, Lei Chen
Summary: In this study, cITLN3 was cloned from common carp and found to play an important role in infection and bacterial stimulation, including bacterial aggregation, inhibition of bacterial proliferation, and potential involvement in intestinal integrity and Toll-like receptors signaling pathway activation.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Physiology
Li'ang Li, Ran Xu, Lingfeng Jiang, Elvis Genbo Xu, Man Wang, Jie Wang, Bo Li, Menghong Hu, Lei Zhang, Youji Wang
Summary: Research on microplastics (MPs) in freshwater organisms is less compared to marine organisms, but the toxic effects of MPs on typical aquaculture fish need to be clarified. Hypoxia complexly interacts with MPs in fish, potentially exacerbating the negative effects on immune factors. Further studies should focus on cellular pathways of immune cells in fish to better understand the impacts of MPs and hypoxia on aquatic organisms.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Chemistry, Medicinal
Ming-Hsiu Yang, Jamie L. Russell, Yuto Mifune, Ying Wang, Hexin Shi, Eva Marie Y. Moresco, Daniel J. Siegwart, Bruce Beutler, Dale L. Boger
Summary: Diprovocims are a new class of TLR agonists that act through the TLR1/TLR2 mechanism, exhibit structure-activity relationships, and have in vivo adjuvant activity. They have potent agonist activity towards human TLR1/TLR2 and can be functionalized without impacting this activity. Improvement in potency and efficacy in the murine system has also been achieved.
JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Fisheries
Kai Lun Wang, Shan Nan Chen, Hui Jun Huo, Pin Nie
Summary: Sixteen Toll-like receptors were identified and characterized in mandarin fish, showing high expression levels in immune-related tissues and mucosa-related tissues and significant up-regulation after stimulation. This study provides valuable information for further research on TLR genes in mandarin fish.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2021)
Article
Immunology
Tao Zheng, Zhuo Song, Jun Qiang, Yifan Tao, Haojun Zhu, Junlei Ma, Pao Xu
Summary: This study investigated the skin responses to transport stress in hybrid yellow catfish through transcriptome and biochemical analyses. The results showed that the skin activated immune signaling pathways and regulated mucus secretion to cope with transport stress.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Fisheries
Tao Zheng, Zhuo Song, Yifan Tao, Jun Qiang, Junlein Ma, Siqi Lu, Pao Xu
Summary: Transport stress has significant effects on the gill tissues and blood of hybrid yellow catfish, altering enzyme activities and blood indicators. However, after a recovery period, some indicators returned to the control levels. These findings provide new insights into the immune response of yellow catfish to transport stress and offer theoretical support for optimizing transportation practices.
FISH & SHELLFISH IMMUNOLOGY
(2022)