Article
Biochemistry & Molecular Biology
Caroline Tyavambiza, Mervin Meyer, Adedoja Dorcas Wusu, Abram Madimabe Madiehe, Samantha Meyer
Summary: The synthesis of silver nanoparticles using plants, especially Cotyledon orbiculata, has resulted in the discovery of effective nanoparticles with antimicrobial and anti-inflammatory activities. The nanoparticles also exhibit antioxidant, growth-promoting, and cell migration properties. Gene expression studies reveal that the water extract of Cotyledon orbiculata and the silver nanoparticles synthesized from this extract promote wound healing by upregulating genes involved in cell proliferation, migration, and growth while downregulating pro-inflammatory genes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Jiaojiao Zhu, Tiao Wen, Shuangquan Qu, Qingnian Li, Biwu Liu, Wenhu Zhou
Summary: This study developed a multifunctional silver nanoclusters-based hydrogel dressing for the treatment of infected wounds. The dressing has the capability to simultaneously fight infection and promote wound healing, showing excellent therapeutic efficacy in a mouse model of infected wounds.
Review
Biology
Mateusz Rybka, Lukasz Mazurek, Marek Konop
Summary: Impaired wound healing is a global problem affecting millions of people, leading to chronic wounds that are susceptible to infections. A shift in the antibacterial treatment paradigm is necessary due to the rising bacterial resistance against antibiotics. Silver nanoparticles have emerged as a promising candidate for the treatment of chronic wounds, with their antimicrobial, anti-inflammatory, and anti-oxidative properties. This review article focuses on experimental and clinical studies on dressings containing silver or silver nanoparticles, highlighting their potential in wound healing.
Review
Biochemistry & Molecular Biology
Manoj Singh, Vanita Thakur, Vikas Kumar, Mayank Raj, Shivani Gupta, Nisha Devi, Sushil Kumar Upadhyay, Marketa Macho, Avik Banerjee, Daniela Ewe, Kumar Saurav
Summary: Wounds are disruptions of skin caused by trauma, surgery, or disease. The healing process is complex and involves inflammatory response, proliferation, and tissue remodeling. Understanding and developing novel therapeutics for these processes are important for achieving maximum recovery. Metal nanoparticles, such as silver, gold, and zinc, have shown promise in wound healing and preventing infections.
Article
Biochemistry & Molecular Biology
Jianrui Qi, Jie Zhang, Hang Jia, Xinyuan Guo, Yuan Yue, Yahong Yuan, Tianli Yue
Summary: This study successfully developed a low-cost antibacterial nanobiomaterial with strong antibacterial activity against Escherichia coli and Staphylococcus aureus, as well as good biocompatibility. In vivo assays confirmed that the nanocomposite not only promoted the healing of wound infection caused by bacteria, but also had no toxic effect on mouse organs.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Materials Science, Multidisciplinary
Kuncham Sudhakar, So Yeon Won, Sung Soo Han
Summary: In this study, a simple method was used to prepare lactose crosslinked gelatin-silver nanocomposite hydrogels with potent antibacterial activities against Escherichia coli and staphylococcus aureus, which have potential applications as wound dressing materials.
Article
Materials Science, Multidisciplinary
Kuncham Sudhakar, So Yeon Won, Sung Soo Han
Summary: A simple one-step method was used to prepare lactose crosslinked gelatin-silver nanocomposite (LGAg) hydrogels, which showed potent antibacterial activities against Escherichia coli and staphylococcus aureus. Characterization results confirmed the hydrogel structure, morphology, and silver nanoparticle formation, suggesting the potential applications of the hydrogel nanocomposites as wound dressing materials.
Article
Biochemistry & Molecular Biology
Jakub Toczek, Marcin Sadlocha, Katarzyna Major, Rafal Stojko
Summary: Silver nanoparticles have antimicrobial properties and can improve the wound healing process. However, there is a need for proper treatment guidelines due to the heterogeneous nature of the published literature on their utilization in wound healing.
Article
Biochemistry & Molecular Biology
Liangfu Zhou, Xixi Zhao, Meixuan Li, Lu Yan, Yao Lu, Chunmei Jiang, Yanlin Liu, Zhongli Pan, Junling Shi
Summary: The study developed a new chitosan composite dressing loaded with iturin-AgNPs, which showed higher antibacterial activity and better promotion of wound healing compared to commercial dressings.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemistry & Molecular Biology
Carolini Mendes, Anand Thirupathi, Rubya Pereira Zaccaron, Maria Eduarda Anastacio Borges Correa, Joao V. S. Bittencourt, Laura de Roch Casagrande, Anadhelly C. S. de Lima, Lara L. de Oliveira, Thiago A. M. de Andrade, Yaodong Gu, Paulo Emilio Feuser, Ricardo A. Machado-de-Avila, Paulo Cesar Lock Silveira
Summary: This study aimed to investigate the effects of iontophoresis and hyaluronic acid (HA) combined with a gold nanoparticle (GNP) solution in an excisional wound model. The results demonstrate that the combination therapy group showed better results in anti-inflammation and tissue repair process compared to the single therapy group.
Article
Pharmacology & Pharmacy
Vajravathi Lakkim, Madhava C. Reddy, VijayaDurga V. V. Lekkala, Veeranjaneya Reddy Lebaka, Mallikarjuna Korivi, Dakshayani Lomada
Summary: Developing an efficient and cost-effective wound-healing substance is urgently needed. Silver nanoparticles synthesized from Azadirachta indica and Catharanthus roseus leaf extracts showed rapid wound healing, increased collagen deposition, and improved antioxidant status in mice. These nanoparticles could be potential natural antioxidants for treating wounds.
Review
Biochemistry & Molecular Biology
Zimkhitha B. Nqakala, Nicole R. S. Sibuyi, Adewale O. Fadaka, Mervin Meyer, Martin O. Onani, Abram M. Madiehe
Summary: Silver nanoparticles (AgNPs) have shown promise in wound healing due to their broad spectrum antimicrobial properties, low toxicity, efficacy at low concentrations, and lack of skin discoloration. They can be combined with other healing-promoting materials to provide optimal healing outcomes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Infectious Diseases
Yogesh Dutt, Ramendra Pati Pandey, Mamta Dutt, Archana Gupta, Arpana Vibhuti, V. Samuel Raj, Chung-Ming Chang, Anjali Priyadarshini
Summary: Silver nanoparticles (AgNPs) have opened up new possibilities in nanobiotechnology due to their large surface area-to-volume ratios and small size. The phytofabrication of AgNPs using plant extracts is a cost-effective and environmentally friendly method with various catalytic activities. The medicinal plant Azadirachta indica possesses phytochemicals that contribute to the pharmaceutical and biological activities of AgNPs. AgNPs have potential applications in drug delivery, bioimaging, cancer treatment, and cell biology.
Article
Nanoscience & Nanotechnology
Reena Badhwar, Bharti Mangla, Yub Raj Neupane, Kushagra Khanna, Harvinder Popli
Summary: The study successfully developed QCT-AgNPs hydrogel as a synergistic treatment for diabetic wounds, demonstrating superior therapeutic efficacy in both in vitro and in vivo studies, thus showing potential for the treatment of diabetic wounds.
Article
Polymer Science
Yasaman Saghafi, Hadi Baharifar, Najmeh Najmoddin, Azadeh Asefnejad, Hassan Maleki, Sayed Mahmoud Sajjadi-Jazi, Alireza Bonkdar, Forough Shams, Kamyar Khoshnevisan
Summary: This study developed gel-based polycaprolactone/chitosan (PCL/CS) nanofibrous dressings containing bromelain (BRO) and silver nanoparticle (Ag NPs) through electrospinning. The resulting nanofibers exhibited uniform morphology without beads, with an average diameter of 176 +/- 63 nm. Adding BRO and Ag NPs improved the tensile strength of the nanofibers and enhanced their antibacterial activity against E. coli and S. aureus bacteria. In vivo experiments demonstrated that the wound healing process was significantly accelerated with the dressings containing BRO and Ag NPs. Therefore, gel-based PCL/CS nanofibrous dressings loaded with BRO and Ag NPs have promising potential for skin wound healing.
Article
Biochemistry & Molecular Biology
Yongbin Li, Zhijia Fang, Xuewei Zhou, Jian Gao, Jingwen Wang, Linru Huang, Yinyan Chen, Lijun Sun, Qi Deng, Ravi Gooneratne
Summary: Cadmium (Cd) pollution in the environment poses a threat to human health as it can easily enter the body through the food chain. Gut microbiota, specifically Escherichia coli, has the potential to reduce Cd-induced intestinal damage and promote Cd excretion. Threonine (Thr) has been found to effectively increase fecal Cd and enhance the abundance of Escherichia-Shigella genus and KF843036_g, leading to reduced Cd accumulation in the liver, kidney, and gut of Cd-exposed mice.
Article
Microbiology
Amal Senevirathne, Jehee Lee, Mahanama De Zoysa, Chamilani Nikapitiya
Summary: Bacteriophage KPP-1 is highly effective against multidrug-resistant K. variicola and can significantly suppress its growth, working synergistically with ampicillin. Further research is needed to develop a strategy for controlling the emerging pathogen K. variicola using bacteriophages such as KPP-1.
Article
Microbiology
Joo-Yeon Lim, Ye-Eun Jung, Hye-Eun Hwang, Cheol-Hee Kim, Nese Basaran-Akgul, Sri Harshini Goli, Steven P. Templeton, Hee-Moon Park
Summary: Deletion of SvfA in Aspergillus nidulans leads to decreased cell wall components, biofilm formation, and protease activity, and increased extracellular signal-regulated kinase (ERK) activation, resulting in reduced virulence.
Editorial Material
Biochemistry & Molecular Biology
Seong-Kyu Choe, Cheol-Hee Kim
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agriculture, Multidisciplinary
Yijia Deng, Rundong Wang, Xuepeng Li, Xiqian Tan, Yuhao Zhang, Ravi Gooneratne, Jianrong Li
Summary: The study examined the effects of fish oil (FO) supplementation on colonic function, immunity, and microbiota in Vibrio parahaemolyticus (Vp)-infected C57BL/6J mice. FO supplementation significantly reduced Vp infection, improved colonic barrier function, and enhanced immune response. It also promoted the growth of beneficial gut bacteria and their metabolites, leading to the alleviation of Vp infection.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Jianzhen Liao, Siyuan Bi, Zhijia Fang, Qi Deng, Yinyan Chen, Lijun Sun, Yongqing Jiang, Linru Huang, Ravi Gooneratne
Summary: Cadmium, a common harmful pollutant, poses a significant threat to human health as it can easily enter the human body through the food chain. Gut microbiota play a key role in cadmium absorption. Docosahexaenoic acid (DHA) is believed to have a potential role in treating cadmium poisoning. This study investigated the therapeutic effect and mechanism of DHA in cadmium-exposed mice by focusing on the gut microbiota. The results showed that DHA increased cadmium excretion and reduced cadmium accumulation in the organs of mice. Restoration of Parabacteroides abundance in the gut microbiota by DHA was also observed. Parabacteroides distasonis, a representative strain, showed capabilities of reducing cadmium toxicity and restoring gut damage caused by cadmium exposure. Furthermore, it significantly reduced cadmium content in various organs and increased cadmium content in feces. The succinic acid produced by P. distasonis played an important role in promoting cadmium excretion. These findings suggest a potential role of P. distasonis in DHA-mediated cadmium excretion in cadmium-exposed mice.
Article
Virology
Amal Senevirathne, E. H. T. Thulshan Jayathilaka, D. K. Haluwana, Kiramage Chathuranga, Mahinda Senevirathne, Ji-Soo Jeong, Tae-Won Kim, Jong-Soo Lee, Mahanama De Zoysa
Summary: This study investigated the antiviral activity of aqueous leaf extract of Costus speciosus (TB100) against influenza A. Pretreatment of TB100 enhanced antiviral activity and inhibited viral replication in murine and human cells. TB100 induced the phosphorylation of transcriptional activators associated with interferon pathways, indicating the activation of antiviral defenses. Safety and efficacy tests confirmed that TB100 was effective against different strains of influenza A. Potential antiviral chemicals, including cinnamic, caffeic, and chlorogenic acids, were identified through high-performance liquid chromatography.
Article
Food Science & Technology
Meifang Hua, Qi Deng, Mei Qiu, Yijia Deng, Lijun Sun, Zhijia Fang, Jianmeng Liao, Jian Zhao, Ravi Gooneratne
Summary: In this study, it was found that iturin A, a cyclic lipopeptide produced by Bacillus subtilis, has a significant inhibitory effect on the growth and synthesis of the T-2 toxin of Fusarium oxysporum. Iturin A was shown to disrupt cell membrane integrity and increase ROS levels. Transcriptomic analysis revealed changes in gene expression related to oxidation-reduction processes, mycelial growth, cell integrity, transmembrane transport, and energy metabolism. This study provides theoretical guidance for the application of iturin A in the preservation of dried aquatic products.
Article
Food Science & Technology
Yijia Deng, Rundong Wang, Yuhao Zhang, Jianrong Li, Ravi Gooneratne
Summary: Free amino acids formed in fermented meat products are crucial for the survival and metabolism of contaminating fungi, and their regulation is mainly controlled by the TORC1-Tap42 signaling pathway. This study investigated the effect of 18 amino acids on the growth, sporulation, toxin synthesis, and gene expression of Fusarium oxysporum. The results showed that certain amino acids positively regulated Fo17 growth and sporulation, while others significantly inhibited growth, not regulated by Tap42. This study contributes to our understanding of amino acid regulation in fermented foods and provides insights into controlling fungal contamination.
Article
Infectious Diseases
Dinusha Chathurangi Rajapaksha, Shan Lakmal Edirisinghe, Chamilani Nikapitiya, Ilson Whang, Mahanama De Zoysa
Summary: In this study, it was found that the antimicrobial peptide octopromycin can inhibit the biofilm and quorum-sensing of Acinetobacter baumannii. Octopromycin reduced biofilm mass, caused a collapse in biofilm architecture, and successfully killed antibiotic-resistant persister cells. It also inhibited violacein production in Chromobacterium violaceum and alginate production, surface movements, and twitching motility of A. baumannii.
Article
Biology
Hannah C. Rudolph, April M. Stafford, Hye-Eun Hwang, Cheol-Hee Kim, Jeremy W. Prokop, Daniel Vogt
Summary: There are several rare genes, including the WAC gene, that are disrupted and contribute to neurological dysfunction. In this study, we explored the role of the WAC protein in brain cells associated with DeSanto-Shinawi syndrome (DESSH), a rare disorder characterized by cranio-facial changes, autism, and attention deficit hyperactivity disorder. We identified novel regions in the WAC protein necessary for its localization to the nucleus and found that the amino-terminus of the protein is responsible for localizing it to distinct cell regions in brain neurons.
Article
Multidisciplinary Sciences
Muhammad Syafiq Akmal Mohd Fahmi, Puspanjali Swain, Amirah Hani Ramli, Wan Norhamidah Wan Ibrahim, Nur Atikah Saleh Hodin, Noraini Abu Bakar, Yee Seng Tan, Siti Munirah Mohd Faudzi, Cheol-Hee Kim
Summary: Epilepsy is a common brain disease and the available antiepileptic drugs often have side effects. In this study, 18 newly designed fluorine-containing pyrrolylated chalcones were tested as potential new AEDs. Compound 8 showed good drug-like properties and binding affinity to GABAA receptor. It effectively reduced convulsive behavior in zebrafish models and had no cardiotoxic effects. Pyrrolylated chalcones could be alternative AEDs and further studies are needed to understand their mechanism of action.
Article
Microbiology
Chamilani Nikapitiya, H. P. S. U. Chandrarathna, Mawalle Kankanamge Hasitha Madhawa Dias, Jehee Lee, Mahanama De Zoysa
Summary: A novel phage (KPP-1) infecting Klebsiella variicola was isolated and characterized from a natural water stream. The phage showed inhibitory effects on the growth of K. variicola both in vitro and in vivo. The study suggests that KPP-1 has the potential to be developed as a biocontrol agent against multidrug-resistant K. variicola.
BRAZILIAN JOURNAL OF MICROBIOLOGY
(2023)
Article
Fisheries
Mawalle Kankanamge Hasitha Madhawa Dias, E. H. T. Thulshan Jayathilaka, Shan Lakmal Edirisinghe, Jae-Woong Lim, Chamilani Nikapitiya, So Young Kang, Ilson Whang, Mahanama De Zoysa
Summary: In this study, octominin, an antimicrobial peptide derived from Octopus minor, was preliminarily tested for its antiviral activity and immune response in fish cells. The results suggest that octominin has potential as an antiviral agent, but further research is needed to understand its mechanism of action.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
J. N. C. Jayasinghe, Ilson Whang, Mahanama De Zoysa
Summary: In this study, Octominin was modified into Octominin II, which showed inhibitory effects on drug-resistant Candida albicans by employing different modes of action and reducing its pathogenicity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
