Article
Biochemistry & Molecular Biology
Silvia Barbon, Elena Stocco, Martina Contran, Federico Facchin, Rafael Boscolo-Berto, Silvia Todros, Deborah Sandrin, Filippo Romanato, Piero Pavan, Veronica Macchi, Vincenzo Vindigni, Franco Bassetto, Raffaele De Caro, Andrea Porzionato
Summary: This study considers the use of cellular human diaphragm as a scaffold material for the treatment of volumetric muscle loss (VML). After decellularization through four detergent-enzymatic protocols, cells, DNA, and muscle fibers were effectively removed, while collagen/elastin and the majority of the glycosaminoglycan component were preserved. Adipose-derived stem cells cultured with decellularized samples demonstrated high viability, indicating the biosafety of the scaffolds. Acellular diaphragmatic grafts treated with SDS+Tergitol (TM) showed better collagen preservation and integrated well with host tissue upon implantation in mice.
Article
Biochemistry & Molecular Biology
Flavia Carton, Dalila Di Francesco, Luca Fusaro, Emma Zanella, Claudio Apostolo, Francesca Oltolina, Diego Cotella, Maria Prat, Francesca Boccafoschi
Summary: The study evaluated the myogenic potential of dECM substrate derived from decellularized bovine pericardium on murine muscle cells and found that dECM could support and enhance the myogenic potential of muscle cells compared to cells grown on a standard plastic surface. This suggests that extracellular matrix proteins could be a promising therapeutic strategy for skeletal muscle tissue engineering.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Naoki Ito, Yuko Miyagoe-Suzuki, Shin'ichi Takeda, Akira Kudo
Summary: In this study, the protective role of periostin, a matricellular protein, in skeletal muscle regeneration was identified. Periostin-null mice showed decreased muscle weight due to the loss of muscle fibers during repeated muscle regeneration. The decrease in CD31-positive blood vessels during muscle regeneration in periostin-null mice suggests that the decreased nutritional supply may be the cause of muscle fiber loss.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Wenhai Cao, Xiaotong Meng, Fangming Cao, Jinpeng Wang, Maowei Yang
Summary: Diabetic non-healing wounds pose a significant burden on patients and society. Platelet-rich plasma (PRP) has been widely used for tissue regeneration and PRP-derived exosomes (PRP-Exos) have shown to be more effective in tissue regeneration. This study investigated the therapeutic potential of PRP-Exos in diabetic wound healing and found that they can promote fibroblast functions and accelerate wound healing through a specific signaling pathway.
Article
Food Science & Technology
Khurshid Ahmad, Jeong-Ho Lim, Eun-Ju Lee, Hee-Jin Chun, Shahid Ali, Syed Sayeed Ahmad, Sibhghatulla Shaikh, Inho Choi
Summary: Cultured meat production is a method that uses tissue engineering techniques to produce animal meat, with key factors including cells, chemical factors, and scaffolds. Scaffolding is crucial for developing organized meat products resembling steaks, with animal-derived polymers like collagen considered the gold standard for producing ECM-like scaffolds.
Article
Immunology
Atomu Yamaguchi, Noriaki Maeshige, Jiawei Yan, Xiaoqi Ma, Mikiko Uemura, Mami Matsuda, Yuya Nishimura, Tomohisa Hasunuma, Hiroyo Kondo, Hidemi Fujino, Zhi-Min Yuan
Summary: This study found that myotube-derived extracellular vesicles (EVs) can suppress macrophage inflammatory responses while activating the IRG1-itaconate pathway. The miRNA composition of EVs may be related to their anti-inflammatory effects.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Wan-Jing Chen, I-Hsuan Lin, Chien-Wei Lee, Yi-Fan Chen
Summary: Aging leads to decline in skeletal muscle function, with reduced regenerative capacity and changes in extracellular matrix structure. This can result in increased collagen deposition and decreased ECM degradation in aged skeletal muscle.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Geriatrics & Gerontology
Yixiao Wang, Yunnan Liu, Siyan Zhang, Na Li, Changyang Xing, Chen Wang, Jia Wang, Mengying Wei, Guodong Yang, Lijun Yuan
Summary: This study demonstrates that exercise-induced extracellular vesicles (EVs) derived from skeletal muscle can improve metabolism and reduce the risk of obesity and atherosclerosis. These EVs can be taken up by major metabolic organs, such as the liver and adipose tissue, and remodel metabolism towards beneficial cardiovascular outcomes. Therapeutic delivery of these EVs or their analogues may hold promise for preventing certain cardiovascular and metabolic diseases.
Article
Biochemistry & Molecular Biology
Ivan J. Vechetti, Bailey D. Peck, Yuan Wen, R. Grace Walton, Taylor R. Valentino, Alexander P. Alimov, Cory M. Dungan, Douglas W. Van Pelt, Ferdinand von Walden, Bjorn Alkner, Charlotte A. Peterson, John J. McCarthy
Summary: Regular physical activity may improve health by releasing factors from skeletal muscle through extracellular vesicles (EVs) containing miR-1, promoting adipose tissue metabolic adaptations. EVs are preferentially taken up by eWAT, enhancing adrenergic signaling and lipolysis, ultimately benefiting overall health.
Article
Biochemistry & Molecular Biology
Yanmiao Fan, Mads Luchow, Adel Badria, Daniel J. Hutchinson, Michael Malkoch
Summary: In this study, placenta powder (PP) made from decellularized human placenta was incorporated into synthetic poly(ethylene glycol) (PEG)-based hydrogels. The PP-incorporated PEG hydrogels showed tunable storage moduli and reduced swelling ratios compared to pristine hydrogels. The hydrogels demonstrated good biocompatibility and the ability to support cell adhesion and proliferation.
Review
Biotechnology & Applied Microbiology
Eugenia Carraro, Lucia Rossi, Edoardo Maghin, Marcella Canton, Martina Piccoli
Summary: This review discusses the importance of generating human skeletal muscle three-dimensional models through tissue engineering approaches, including the study of the most severe myopathies and the comparison of different in vitro models, as well as their effectiveness in simulating disease mechanisms and investigating therapeutic effects.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Engineering, Biomedical
Charlot Philips, Lisanne Terrie, Lieven Thorrez
Summary: A review is presented on the progress and challenges in skeletal muscle decellularization, discussing different decellularization methods and applications of the scaffolds. Decellularized skeletal muscle has been primarily studied for regenerative purposes, focusing on tissue-specific morphological features and biochemical cues for muscle regeneration. However, this review expands the potential applications of decellularized skeletal muscle to demonstrate its versatility as a biomaterial.
Article
Neurosciences
Sarah E. Brashear, Ross P. Wohlgemuth, Gabriella Gonzalez, Lucas R. Smith
Summary: The amount of fibrotic material in muscle is related to contractile function and passive stiffness, but excessive fibrosis does not directly lead to decreased muscle function. Anti-fibrotic therapies should target collagen architecture, particularly large collagen fibers and their alignment, to enhance muscle function and reduce excessive stiffness.
JOURNAL OF PHYSIOLOGY-LONDON
(2021)
Article
Engineering, Biomedical
Stephan Kohn, Kay Leichsenring, Ramachandra Kuravi, Alexander E. Ehret, Markus Boel
Summary: This study reports the first comprehensive dataset on the anisotropic mechanical properties of isolated endo- and perimysial extracellular matrix of skeletal muscle, along with protocols for preparation and testing. The results contribute to understanding muscle mechanics and load transfer mechanisms within muscle tissue, and are relevant to constitutive formulation of muscle tissue and tissue-engineering grafts.
ACTA BIOMATERIALIA
(2021)
Article
Geriatrics & Gerontology
Zachary R. Hettinger, Kyoko Hamagata, Amy L. Confides, Marcus M. Lawrence, Benjamin F. Miller, Timothy A. Butterfield, Esther E. Dupont-Versteegden
Summary: The study suggests that older adults have difficulty recovering lost muscle mass following disuse atrophy, with mechanotherapy showing potential for enhancing recovery but with reduced effectiveness in older adults compared to adults. Age-related transverse muscle stiffness may hinder the growth response to mechanotherapy, indicating the importance of considering age-related factors in designing rehabilitation strategies for muscle disuse atrophy.
JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES
(2021)
Article
Orthopedics
Jeffrey R. Talarek, Alex N. Piacentini, Alexis C. Konja, Susumu Wada, Jacob B. Swanson, Samuel C. Nussenzweig, Joshua S. Dines, Scott A. Rodeo, Christopher L. Mendias
JOURNAL OF ORTHOPAEDIC RESEARCH
(2020)
Article
Physiology
Andrew C. Noah, Thomas M. Li, Leandro M. Martinez, Susumu Wada, Jacob B. Swanson, Nathaniel P. Disser, Kristoffer B. Sugg, Scott A. Rodeo, Theresa T. Lu, Christopher L. Mendias
JOURNAL OF APPLIED PHYSIOLOGY
(2020)
Article
Neurosciences
Nathaniel P. Disser, Gregory C. Ghahramani, Jacob B. Swanson, Susumu Wada, Max L. Chao, Scott A. Rodeo, David J. Oliver, Christopher L. Mendias
JOURNAL OF PHYSIOLOGY-LONDON
(2020)
Article
Orthopedics
Michael T. Curran, Asheesh Bedi, Christopher L. Mendias, Edward M. Wojtys, Megan Kujawa, Riann M. Palmieri-Smith
AMERICAN JOURNAL OF SPORTS MEDICINE
(2020)
Article
Orthopedics
Christopher L. Mendias, Elizabeth R. Sibilsky Enselman, Adam M. Olszewski, Jonathan P. Gumucio, Daniel L. Edon, Maxwell A. Konnaris, James E. Carpenter, Tariq M. Awan, Jon A. Jacobson, Joel J. Gagnier, Ariel L. Barkan, Asheesh Bedi
AMERICAN JOURNAL OF SPORTS MEDICINE
(2020)
Article
Orthopedics
Nathaniel P. Disser, Andrea J. De Micheli, Martin M. Schonk, Maxwell A. Konnaris, Alexander N. Piacentini, Daniel L. Edon, Brett G. Toresdahl, Scott A. Rodeo, Ellen K. Casey, Christopher L. Mendias
JOURNAL OF BONE AND JOINT SURGERY-AMERICAN VOLUME
(2020)
Article
Orthopedics
Drake G. LeBrun, Maxwell A. Konnaris, Gregory C. Ghahramani, Ajay Premkumar, Chris J. DeFrancesco, Jordan A. Gruskay, Aleksey Dvorzhinskiy, Milan S. Sandhu, Elan M. Goldwyn, Christopher L. Mendias, William M. Ricci
JOURNAL OF ORTHOPAEDIC TRAUMA
(2020)
Article
Cell Biology
Douglas W. Van Pelt, Yalda A. Kharaz, Dylan C. Sarver, Logan R. Eckhardt, Justin T. Dzierzawski, Nathaniel P. Disser, Alex N. Piacentini, Eithne Comerford, Brian McDonagh, Christopher L. Mendias
Summary: This study utilized multiomic approaches to investigate the pathological changes in Duchenne muscular dystrophy at the transcriptome, proteome, metabolome, and lipidome levels. Despite gross pathological changes in muscles of mdx/mTR mice, there were no differences in muscle fiber contractility compared to wild type mice. There was a moderate agreement between the proteome and transcriptome but also significant differences, with notable changes in markers of muscle metabolism observed in dystrophic muscles.
CONNECTIVE TISSUE RESEARCH
(2021)
Article
Medicine, Research & Experimental
Jonathan P. Gumucio, Martin M. Schonk, Yalda A. Kharaz, Eithne Comerford, Christopher L. Mendias
Article
Cell Biology
Andrea J. De Micheli, Jacob B. Swanson, Nathaniel P. Disser, Leandro M. Martinez, Nicholas R. Walker, David J. Oliver, Benjamin D. Cosgrove, Christopher L. Mendias
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2020)
Article
Anatomy & Morphology
Nathan S. Jeffery, Dylan C. Sarver, Christopher L. Mendias
Summary: This study investigates the competing spatial demands of brain and masticatory muscle growth within hypermuscular myostatin-deficient mouse model and computational simulations, exploring the network linking single genes to multiple phenotypic outcomes. Findings reveal different muscle and brain volume changes in myostatin-deficient mice at different growth stages, and simulations show skeletal morphological changes associated with muscle expansion and endocranial expansion.
JOURNAL OF ANATOMY
(2021)
Article
Biochemistry & Molecular Biology
Haiyin Li, Antonion Korcari, David Ciufo, Christopher L. L. Mendias, Scott A. A. Rodeo, Mark R. R. Buckley, Alayna E. E. Loiselle, Geoffrey S. S. Pitt, Chike Cao
Summary: This study found that Ca2+ signaling through Ca(V)1.2 voltage-gated Ca2+ channel plays a role in tendon formation. Ca(V)1.2 is highly expressed in developing tendons but downregulated in adult homeostasis. Mutant tendons with increased Ca2+ signaling display hypertrophic characteristics, increased collagen fibrillogenesis, and alterations in ECM proteins and growth factors.
Article
Sport Sciences
Jonathan S. Yu, David M. Dare, Daniel Edon, Alec L. Sinatro, Dylan C. Sarver, Scott Rodeo, Joshua S. Dines, Christopher L. Mendias
Summary: This exploratory study aimed to investigate the relationship between biomarkers of cartilage turnover and inflammation and specific shoulder lesions in patients with shoulder instability. The findings suggest that inflammatory biomarkers were not associated with specific shoulder lesions, while biomarkers of cartilage turnover were only elevated in Hill-Sachs lesions.
TRANSLATIONAL SPORTS MEDICINE
(2022)
Article
Orthopedics
Jordan A. Gruskay, Aleksey Dvorzhinskiy, Maxwell A. Konnaris, Drake G. LeBrun, Gregory C. Ghahramani, Ajay Premkumar, Christopher J. DeFrancesco, Christopher L. Mendias, William M. Ricci
JOURNAL OF BONE AND JOINT SURGERY-AMERICAN VOLUME
(2020)
Article
Physiology
Dylan C. Sarver, Kristoffer B. Sugg, Jeffrey R. Talarek, Jacob B. Swanson, David J. Oliver, Aaron C. Hinken, Henning F. Kramer, Christopher L. Mendias
PHYSIOLOGICAL REPORTS
(2019)