Journal
FEBS LETTERS
Volume 591, Issue 15, Pages 2251-2260Publisher
WILEY
DOI: 10.1002/1873-3468.12721
Keywords
chloride channel; mean-variance analysis; mitochondria
Funding
- Scientific Grant Agency of MESRaS and SAS [VEGA 2/0094/12, VEGA 2/0146/16, VEGA 2/0095/15]
- Slovak Research and Development Agency [APVV-15-0371]
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Recently, it has been discovered that isoforms of intracellular chloride channels (CLIC) are present in cardiac mitochondria. By reconstituting rat cardiac mitochondrial chloride channels into bilayer lipid membranes, we detected three equally separated subconductance states with conductance increment of 45 pS and < 2% occupancy. The observed rare events of channel decomposition into substates, accompanied by disrupted gating, provide an insight into channel quaternary structure. Our findings suggest that the observed channels work as four functionally coupled subunits with synchronized gating. We discuss the putative connection of channel activity from native mitochondria with the recombinant CLIC channels. However, conclusive evidence is needed to prove this connection.
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