Review
Cell Biology
Natallia Makarava, Ilia V. Baskakov
Summary: This review article discusses the role of sialylation in prion diseases. It demonstrates that the sialylation status of prion protein is associated with the spread of PrPSc in the brain and the outcomes of prion infection. Moreover, sialic acid residues also play a role in prion replication, cross-species transmission, and strain competition.
CELL AND TISSUE RESEARCH
(2023)
Article
Oncology
Siyaporn Putthisen, Atit Silsirivanit, Orasa Panawan, Akiko Niibori-Nambu, Yuki Nishiyama-Ikeda, Prasertsri Ma-In, Sukanya Luang, Kunimasa Ohta, Kanha Muisuk, Sopit Wongkham, Norie Araki
Summary: A new type of glioma stem cell has been discovered, which expresses a unique sialic acid-modified glycan. This glycan is highly expressed in glioma stem cells and its inhibition can induce apoptosis of the stem cells, making it a potential marker and therapeutic target for glioma treatment.
EXPERIMENTAL CELL RESEARCH
(2022)
Article
Health Care Sciences & Services
Mircea Tampa, Ilinca Nicolae, Cristina Iulia Mitran, Madalina Irina Mitran, Cosmin Ene, Clara Matei, Simona Roxana Georgescu, Corina Daniela Ene
Summary: The study found significantly increased levels of serum sialic acid in patients with cutaneous squamous cell carcinoma, correlating with tumor aggressiveness, while the sialic acid levels in patients with actinic keratoses only showed significant differences compared to the control group.
JOURNAL OF PERSONALIZED MEDICINE
(2021)
Article
Chemistry, Multidisciplinary
Sara Bertuzzi, Francesca Peccati, Sonia Serna, Raik Artschwager, Simona Notova, Michel Thepaut, Gonzalo Jimenez-Oses, Franck Fieschi, Niels C. Reichardt, Jesus Jimenez-Barbero, Ana Arda
Summary: The molecular recognition features of LSECtin towards asymmetric N-glycans were scrutinized by NMR and compared to glycan microarrays. It was found that both asymmetric LDN3 and LDN6 N-glycans are recognized by LSECtin with similar affinities in solution, contrary to the results obtained on microarrays, where only LDN6 was efficiently recognized by the lectin.
ACS CENTRAL SCIENCE
(2022)
Article
Biochemical Research Methods
Dinko Soic, Toma Keser, Jerko Stambuk, Domagoj Kifer, Flemming Pociot, Gordan Lauc, Grant Morahan, Mislav Novokmet, Olga Gornik
Summary: Recent studies have shown that children with early-onset type 1 diabetes (T1D) have a higher proportion of oligomannose glycans in their plasma protein N-glycome compared to healthy siblings. This study aimed to characterize the glycosylation profile of complement component C3 in T1D and found significant changes in C3 N-glycan profiles associated with T1D. The study also confirmed the involvement of C3 N-glycome in T1D development.
MOLECULAR & CELLULAR PROTEOMICS
(2022)
Article
Microbiology
Natali Nakic, Thanh Hoa Tran, Mislav Novokmet, Olivier Andreoletti, Gordan Lauc, Giuseppe Legname
Summary: This article discusses the controversy surrounding prion diseases and the impact of the structure of the prion protein and its glycan structures on the disease. By comparing the glycan structures of two different sheep prion strains, it was found that there were no major differences, suggesting that glycans may not be responsible for the differences in the two analyzed prion strains.
Review
Chemistry, Multidisciplinary
Xiaoya Zhao, Yan Huang, Siai Zhou, Jiaming Ao, Hui Cai, Katsunori Tanaka, Yukishige Ito, Akihiro Ishiwata, Feiqing Ding
Summary: Glycosylation is a major form of protein post-translational modification, and N-glycans play a crucial role in intercellular interaction and immune function. Efficient methods to provide homogeneous complex-type N-glycans have been urgently needed for analytical and medicinal purposes. Significant progress has been made in both chemical and chemoenzymatic strategies for the synthesis of N-glycans.
FRONTIERS IN CHEMISTRY
(2022)
Review
Pharmacology & Pharmacy
Renato Mastrangeli, Maria Concetta Audino, Wolf Palinsky, Herve Broly, Horst Bierau
Summary: The review discusses the structural and functional considerations of Neu5Gc-containing glycans in therapeutic recombinant proteins, including their potential impact on drug clearance, recognition by pre-existing antibodies, and recent hypotheses regarding tolerance to low Neu5Gc levels. Recommendations are provided for standardization of analysis and reporting, relevant analytical aspects for risk assessment associated with Neu5Gc-containing biotherapeutics, and approaches to minimize Neu5Gc incorporation in recombinant protein manufacturing.
TRENDS IN PHARMACOLOGICAL SCIENCES
(2021)
Article
Microbiology
Kotaro Ishida, Hirokazu Yagi, Yukinari Kato, Eiji Morita
Summary: This study investigates the role of N-glycosylation of flaviviral structural proteins in viral particle formation and secretion and finds that the amount of glycosylation regulates the release of subviral particles. Glycosylation mainly affects pre-Golgi transport.
Review
Biochemistry & Molecular Biology
Sarah Okun, Allyson Peek, Suleiman A. Igdoura
Summary: Sialidases are enzymes that are present in various organisms, including viruses, bacteria, fungi, avians, and mammals. Mammalian sialidases exhibit differences in specificity, pH optimum, subcellular localization, and tissue expression. This review focuses on NEU4 sialidase and its impact on multiple physiological and cellular processes, including cancer, lysosomal storage disease, and cellular differentiation. Modulating NEU4 expression may play a crucial role in the breakdown of sialylated glycoconjugates and the regulation of important cellular events.
Article
Multidisciplinary Sciences
Chatchai Phoomak, Wei Cui, Thomas J. Hayman, Seok-Ho Yu, Peng Zhao, Lance Wells, Richard Steet, Joseph N. Contessa
Summary: This study demonstrates that cells lacking the SSR3 or SSR4 subunits can restore N-glycosylation over time and reduce the transcriptional signature of ER stress. The protein levels of the SSR3 subunit are dependent on ER stress and UBE2J1, coordinating upstream N-glycosylation proficiency with downstream ER-associated degradation and proteostasis. The TRAP complex plays a regulatory role in maintaining ER glycoprotein quality control under stress conditions in both non-transformed and tumor cells.
Article
Neurosciences
Laura Cracco, Gianfranco Puoti, Antonio Cornacchia, Katie Glisic, Seong-Ki Lee, Zerui Wang, Mark L. Cohen, Brian S. Appleby, Ignazio Cali
Summary: This study investigates the biochemical properties and phenotypic expression of sCJD MV1 and MV2 cases. The MV2 subtype shows four different histotypes, with the highest prevalence for 2C-K and the lowest for 2C-PL and 2K. MV1 cases mainly exhibit the 1C histotype, with a rapid disease progression and short survival time.
ACTA NEUROPATHOLOGICA COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Claudia F. Lossio, Vinicius J. S. Osterne, Vanir R. Pinto-Junior, Simin Chen, Messias V. Oliveira, Joost Verduijn, Isabel Verbeke, Sonia Serna, Niels C. Reichardt, Andre Skirtach, Benildo S. Cavada, Els J. M. Van Damme, Kyria S. Nascimento
Summary: This study investigated the structural properties and anticancer potential of a mannose-specific lectin from Canavalia villosa seeds. The lectin showed specific binding activity to carbohydrates and inhibited cancer cell proliferation by activating autophagic and apoptotic pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Jialin Liu, Bo Cheng, Xinqi Fan, Xinyue Zhou, Jiankun Wang, Wen Zhou, Hengyu Li, Wenfeng Zeng, Pengyuan Yang, Xing Chen
Summary: Proteins are extensively modified with glycans of different structures, making it challenging to map the protein glycosylation landscape. This study presents Click-iG, a method that enables the simultaneous enrichment and profiling of three types of intact glycopeptides using metabolic labeling of glycans, optimized mass spectrometry, and tailored software analysis. Click-iG was able to identify thousands of intact glycosites in cell lines and living mice, providing comprehensive coverage of the protein glycosylation landscape and laying the foundation for investigating crosstalk between different glycosylation pathways.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Multidisciplinary Sciences
Ngan T. B. Nguyen, Jianer Lin, Shi Jie Tay, Mariati, Jessna Yeo, Terry Nguyen-Khuong, Yuansheng Yang
Summary: In this study, the researchers manipulated the N-glycan structures on therapeutic antibodies by overexpressing various glycosyltransferase genes in CHO cells, resulting in antibodies with different N-linked glycan structures. This approach opens up new possibilities for developing novel recombinant antibodies for addressing specific therapeutic applications.
SCIENTIFIC REPORTS
(2021)