Article
Biochemistry & Molecular Biology
Michael G. McCoy, Daniel Perez-Cremades, Nathan Belkin, Wenhui Peng, Bofang Zhang, Jingshu Chen, Madhur Sachan, A. K. M. Khyrul Wara, Rulin Zhuang, Henry S. Cheng, Mark W. Feinberg
Summary: This study demonstrates that a 4-miRNA cassette can reprogram vascular smooth muscle cells into endothelial cells, and these induced cells exhibit high similarity to native endothelial cells. Injected induced cells even restore blood flow faster than conventional endothelial cells in a mouse model, suggesting the potential of this approach for vascular disease therapy.
Article
Biochemistry & Molecular Biology
Yanpeng Lv, Zhikui Feng, Shuo Chen, Xian Cheng, Jianhua Zhang, Chenguo Yao
Summary: This study investigated the electroporation effect on endothelial cells in normal and tumor blood vessels using a multilayer dielectric model. It found that vascular smooth muscle cells in normal blood vessels may provide protection against electroporation, while the lack of these cells in tumor blood vessels led to a stronger electroporation effect on endothelial cells. This study may help explain why the structure of endothelial cells in normal blood vessels is preserved during electroporation treatment and how ECT or IRE may selectively damage tumor blood vessels and cells while preserving normal blood vessels.
BIOELECTROCHEMISTRY
(2022)
Article
Immunology
Fahim Syed, Wei Li, Ryan F. Relich, Patrick M. Russell, Shanxiang Zhang, Michelle K. Zimmerman, Qigui Yu
Summary: The study found that COVID-19 patients exhibit a unique inflammatory profile, with high levels of MMP-1 and hyperactivation of endothelial cells (ECs) associated with disease severity.
JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Biochemistry & Molecular Biology
Daniel Perez-Cremades, Ana B. Paes, Xavier Vidal-Gomez, Ana Mompeon, Carlos Hermenegildo, Susana Novella
Summary: This study identified a comprehensive regulatory network controlling transcriptomic changes in endothelial cells exposed to estradiol, highlighting the important role of estrogen in regulating endothelial function. The analysis revealed interactions between miRNAs and transcription factors, as well as downstream target genes, involved in key signaling pathways such as hypoxia signaling and cell adhesion.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Gastroenterology & Hepatology
Andrzej S. Tarnawski, Amrita Ahluwalia
Summary: COVID-19 affects various organs including the respiratory system, gastrointestinal tract, liver, pancreas, etc. Blood vessels serve as conduits for viral dissemination, with endothelial cells playing a critical role. Current treatments, such as molnupiravir and paxlovid, show significant efficacy in controlling severe COVID-19 infection.
WORLD JOURNAL OF GASTROENTEROLOGY
(2022)
Article
Cell Biology
Bart Weijts, Iftach Shaked, Mark Ginsberg, David Kleinfeld, Catherine Robin, David Traver
Summary: The formation of large blood vessels relies on the presence of a scaffold structure, which traps erythrocytes to create a rigid and stable vessel. Experimental studies show that severing these scaffold structures results in vessel collapse and remodeling defects, emphasizing the importance of struts in maintaining the patency of large vessels during their formation.
NATURE CELL BIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Fengjie Guo, Zhipeng Ren, Dongxu Liu, Linghui Wang, Xiaobin Hou, Wen Chen
Summary: Regulatory T cells (Treg cells) inhibit intimal hyperplasia of tissue-engineered blood vessels (TEBVs) in diabetic pigs by promoting endothelial progenitor cells (EPCs) mobilization, anti-inflammatory action, and cellular protection.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Physiology
Jie Yang, Hong Xu, Kejie Chen, Danni Zheng, Shuang Liu, Xia Zhou, Yapeng Lin, Hang Cheng, Qin Luo, Min Yang, Xiaoyan Yan, Junli Hao
Summary: This study discovered that activated platelets can modulate the expression of endothelin 1 and vascular endothelial growth factor A in endothelial cells through the release of miR-200a-3p-enriched microvesicles. This provides a potential predictive biomarker and therapeutic strategy for atherosclerosis and acute ischemic stroke.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Cardiac & Cardiovascular Systems
Stefania Momi, Emanuela Falcinelli, Eleonora Petito, Giulia Ciarrocca Taranta, Alice Ossoli, Paolo Gresele
Summary: Platelets containing and releasing MMP-2 play a crucial role in the development and progression of atherosclerosis. Targeting platelet MMP-2 may provide a new approach for preventing atherosclerosis.
EUROPEAN HEART JOURNAL
(2022)
Article
Cell Biology
Yiqiong Yang, Jing Wang, Yu Zhang, Xiuxiu Hu, Li Li, Pingsheng Chen
Summary: The study found that upregulation of Rab7 under hypoxia conditions can reverse the inhibitory effects on the survival and proliferation of human microvascular endothelial cells (HMEC-1 cells), as well as the inhibitory effects on the migration and tube length of HMECs.
Article
Biochemistry & Molecular Biology
Jonathan Pratt, Khadidja Haidara, Borhane Annabi
Summary: Modulations in cell surface receptor ectodomain proteolytic shedding impact on receptor function and cancer biomarker expression. LRP-1-mediated Angiopep-2 internalization is influenced by MT1-MMP expression. Inhibitors of MMP increase An2 internalization, while MT1-MMP overexpression reduces it. Combined administration of MMP inhibitors and An2-drug conjugates may enhance the treatment of high-MT1-MMP-expressing brain tumors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biology
Charles E. MacKay, Miranda Floen, M. Dennis Leo, Raquibul Hasan, Tessa A. C. Garrud, Carlos Fernandez-Pena, Purnima Singh, Kafait U. Malik, Jonathan H. Jaggar
Summary: This study reveals that PC-1 plays a regulatory role in arterial contractility through its interaction with PC-2 in endothelial cells. Flow stimulation leads to the activation of PC-1/PC-2 clusters, resulting in the activation of eNOS, IK channels, and SK channels, as well as vasodilation and reduction in blood pressure.
Article
Cell & Tissue Engineering
Ru-Sheng Liu, Bin Li, Wen-Dong Li, Xiao-Long Du, Xiao-Qiang Li
Summary: This study investigated the effects and mechanisms of miRNA-130a in human endothelial progenitor cells (EPCs) involved in deep vein thrombosis (DVT). The results showed that miRNA-130a promotes migration, invasion, and tube formation of EPCs by positively regulating the expression of MMP-1 through Akt/PI3K/mTOR signaling pathway.
JOURNAL OF BIOMATERIALS AND TISSUE ENGINEERING
(2022)
Review
Polymer Science
Vera S. Chernonosova, Pavel P. Laktionov
Summary: This article reviews current research on the significance of scaffold architecture in interactions between cells and blood. By studying the fiber characteristics, porosity, and surface roughness, the effects on cellular behavior and blood interactions can be better understood to improve the design of electrospun vascular grafts.
Article
Chemistry, Multidisciplinary
Zachary P. Lin, Wayne Ngo, Stefan M. Mladjenovic, Jamie L. Y. Wu, Warren C. W. Chan
Summary: Nanoparticles traveling through blood vessels may interact with platelet factor 4 proteins released from activated platelets, affecting their surface chemistry and cellular interactions.
Article
Chemistry, Analytical
Frederike Nordmeier, Adrian A. Doerr, Stefan Potente, Nadja Walle, Matthias W. Laschke, Michael D. Menger, Peter H. Schmidt, Markus R. Meyer, Nadine Schaefer
Summary: The interpretation of analytical results in forensic postmortem cases is often challenging, especially due to possible postmortem redistribution phenomena. A controlled toxicokinetic study on pigs was performed to examine the tissue distribution and possible redistribution of synthetic opioids U-47700 and tramadol. The study found that these substances are only slightly prone to redistribution, and central blood might be the best matrix for quantification.
JOURNAL OF ANALYTICAL TOXICOLOGY
(2023)
Article
Surgery
Andrea Weinzierl, Yves Harder, Michael D. Menger, Matthias W. Laschke
Summary: This study found that intermittent fasting can effectively prevent flap necrosis by increasing angiogenesis and reducing inflammatory responses.
PLASTIC AND RECONSTRUCTIVE SURGERY
(2023)
Article
Medicine, Research & Experimental
Vivien Becker, Xu Yuan, Anne S. Boewe, Emmanuel Ampofo, Elke Ebert, Johannes Hohneck, Rainer M. Bohle, Eckart Meese, Yingjun Zhao, Michael D. Menger, Matthias W. Laschke, Yuan Gu
Summary: The downregulation of miR-186 in endothelial cells (ECs) mediates hypoxia-stimulated angiogenesis in non-small cell lung cancer (NSCLC) by upregulating protein kinase C alpha (PKCa). In vitro and in vivo experiments showed that miR-186 mimic inhibited ECs' angiogenic activity, while miR-186 inhibitor exerted pro-angiogenic effects. The findings suggest that targeting miR-186 and PKCa could be a potential strategy for NSCLC treatment.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2023)
Article
Hematology
Laura Hertz, Daniel Flormann, Lutz Birnbaumer, Christian Wagner, Matthias W. Laschke, Lars Kaestner
Summary: The protein TRPC6 is present in mouse red blood cells but absent in human red blood cells. Transfusion experiments showed that TRPC6 could be restored in mice within 10 days. Mechanical stimulation experiments suggested that protein transfer to red blood cells can occur in the absence of translational machinery. This protein transfer mechanism has potential therapeutic applications for diseases involving red blood cells.
Article
Hematology
Lynn M. M. Knowles, Carolin Wolter, Michael D. D. Menger, Matthias W. W. Laschke, Lars Beyer, Ulrich Gruen, Hermann Eichler, Jan Pilch
Summary: To determine the presence of recurrent inflammation in hemophilia, the acute-phase response was evaluated in hemophilia A and B patients. Elevated levels of IL-6, CRP, and LBP were observed in the entire cohort of hemophilia patients, with a stronger increase in obese patients. Nonobese patients with recent bleeding events showed a spike in IL-6, CRP, and LBP, along with an increase in sIL6Ra. IL-6 was identified as a marker of bleeding in hemophilia.
THROMBOSIS AND HAEMOSTASIS
(2023)
Article
Cell & Tissue Engineering
Ettore Limido, Andrea Weinzierl, Yves Harder, Michael D. Menger, Matthias W. Laschke
Summary: This review provides a comprehensive overview of strategies for enhancing vascularization of fat grafts in preclinical models. These strategies, if successfully applied in clinical practice, could greatly improve the outcomes of fat transplantation.
TISSUE ENGINEERING PART B-REVIEWS
(2023)
Article
Oncology
Antonios E. Spiliotis, Sebastian Hollaender, Jeannette Rudzitis-Auth, Gudrun Wagenpfeil, Robert Eisele, Spyridon Nika, Orestis Mallis Kyriakides, Matthias W. Laschke, Michael D. Menger, Matthias Glanemann, Gereon Gaebelein
Summary: Electrochemotherapy (ECT) is an effective treatment for hepatic tumors that combines the administration of chemotherapeutic agents with well-dosed electric pulses for cell membrane reversible electroporation. It reduces tumor oxygenation, increases tumor necrosis, and reduces tumor vascularization compared to reversible electroporation and chemotherapy. ECT is a valuable option for treating hepatic tumors with necrosis rates >85% five days post-treatment.
Review
Cell Biology
Yuan Gu, Maximilian A. Becker, Luisa Mueller, Katharina Reuss, Frederik Umlauf, Tianci Tang, Michael D. Menger, Matthias W. Laschke
Summary: Tumor endothelial cells (TECs) are crucial for tumor angiogenesis, growth and metastasis, and small non-coding microRNAs (miRNAs) play a significant role in regulating TEC function and blood vessel formation. This review provides an overview of the regulatory mechanisms and effects of endothelial miRNAs, highlighting their involvement in fundamental angiogenesis-related signaling pathways. Targeting endothelial miRNAs represents a potential strategy for anti-angiogenic cancer therapy, but further research is needed to understand their networks and develop specific delivery technologies.
Article
Cell Biology
Mohammed Nouaman, Alexis Darras, Thomas John, Greta Simionato, Minke A. E. Rab, Richard van Wijk, Matthias W. Laschke, Lars Kaestner, Christian Wagner, Steffen M. Recktenwald
Summary: The deformability of RBCs is crucial for blood flow in the microcirculatory system. The age of RBCs affects their physical properties and their shape in microfluidic channels. Our study reveals that older RBCs form more stable symmetric croissant shapes, while chemically rigidified RBCs show suppressed formation of this shape class. This research provides insights into the distinct effects of age-related changes in cell properties on the flow behavior of RBCs in confined flows.
Article
Biochemistry & Molecular Biology
Andrea Weinzierl, Yves Harder, Daniel Schmauss, Michael D. Menger, Matthias W. Laschke
Summary: This study demonstrates that microvascular fragments (MVF) derived from adipose tissue can reduce flap necrosis by increasing angiogenesis, improving tissue perfusion, and suppressing apoptosis. These findings provide a promising strategy for reducing ischemia-induced flap necrosis in clinical practice by injecting MVF.
Article
Biotechnology & Applied Microbiology
Maximilian M. Menger, Ruben Manuschewski, Sabrina Ehnert, Mika F. Rollmann, Tanja C. Maisenbacher, Anne L. Tobias, Michael D. Menger, Matthias W. Laschke, Tina Histing
Summary: With a growing elderly population, trauma and reconstructive surgery face challenges in treating geriatric patients. The impact of aging on bone healing remains controversial, prompting an investigation on fracture healing in young adult and aged CD-1 mice. The study revealed delayed callus remodeling and impaired bending stiffness in aged animals, suggesting delayed fracture healing. However, the overall healing capacity of fractured femora was not affected by aging.
BIOENGINEERING-BASEL
(2023)
Article
Cell & Tissue Engineering
Friederike C. Messner, Wolfgang Metzger, Julia E. Marschall, Caroline Bickelmann, Michael D. Menger, Matthias W. Laschke
Summary: MVF isolates generated by short-term enzymatic digestion are beneficial for tissue defect vascularization, isolates from obese mice lack connective tissue and exhibit improved in vivo vascularization.
TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2023)
Article
Nutrition & Dietetics
Andrea Weinzierl, Maximilian Coerper, Yves Harder, Michael D. Menger, Matthias W. Laschke
Summary: This study demonstrates that a 30% reduction in caloric intake effectively prevents flap necrosis by maintaining microperfusion and inhibiting inflammation under ischemic stress.
Article
Obstetrics & Gynecology
Gerhard Leyendecker, Ludwig Wildt, Matthias W. Laschke, Gerhard Mall
Summary: This article presents a new concept of uterine adenomyosis, peritoneal and peripheral endometriosis, suggesting that tissue injury and repair play a key role in the pathogenesis. The disease may develop after onset of biomechanical injury in individuals with uterine hypercontractility, and preventive measures can be taken for high-risk individuals.
ARCHIVES OF GYNECOLOGY AND OBSTETRICS
(2023)