Article
Biochemistry & Molecular Biology
Neha S. Dole, Jihee Yoon, David A. Monteiro, Jason Yang, Courtney M. Mazur, Serra Kaya, Cassandra D. Belair, Tamara Alliston
Summary: This study investigated a microRNA-dependent mechanism mediating the crosstalk between TGF-β and Wnt signaling in osteocytes exposed to fluid shear stress, revealing that miR-100 integrates the functions of these two essential pathways by antagonizing Wnt signaling.
Article
Biochemistry & Molecular Biology
Bai Zhang, Xueyi Li, Kai Tang, Ying Xin, Guanshuo Hu, Yufan Zheng, Keming Li, Cunyu Zhang, Youhua Tan
Summary: Tumor cells with the ability to adhere to specific organ endothelium exhibit enhanced metastatic tropism to that target organ.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Anushree Dwivedi, Patrick A. Kiely, David A. Hoey
Summary: Bone is a common site for breast cancer metastasis, with mechanically stimulated osteocytes releasing factors that enhance cancer cell proliferation and migration. The secretome of mechanically activated osteocytes contains specific cytokines that potentially drive breast cancer metastasis to bone.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Cell Biology
Alper D. Ozkan, Tina Gettas, Audrey Sogata, Wynn Phaychanpheng, Miou Zhou, Jerome J. Lacroix
Summary: The study developed a genetically encoded fluorescent reporter, iGlow, by inserting a fluorescent protein into the third intracellular loop of GPR68, which responds to physiological GPR68 activators and synthetic agonists with specificity. iGlow activation is unaffected by pharmacological modulation of downstream G-protein signaling or disruption of actin filaments, providing a new tool for investigating GPR68-dependent signaling in health and disease.
JOURNAL OF CELL SCIENCE
(2021)
Article
Biology
Sen Zhao, Zebin Chen, Taiyang Li, Qing Sun, Huijie Leng, Bo Huo
Summary: This study investigated the characteristics of fluid flow at different scales in cancellous bone in rat femurs, as well as the effects of osteoporosis and loading frequency. The results showed that the wall fluid shear stress (FSS) around osteocytes exhibited a linear relationship with loading frequency and was smaller in the osteoporotic group. This study provides important insights into bone remodeling under cyclic loading and the development of strategies for osteoporosis treatment.
COMPUTERS IN BIOLOGY AND MEDICINE
(2023)
Article
Oncology
Thomas R. Coughlin, Ali Sana, Kevin Voss, Abhilash Gadi, Upal Basu-Roy, Caroline M. Curtin, Alka Mansukhani, Oran D. Kennedy
Summary: Primary bone cancers like osteosarcoma are driven by bone cells with mutations, and their behavior can be influenced by mechanical stimuli in the tumor microenvironment. Fluid flow over the cells' surface was found to change the behavior of osteosarcoma cells, potentially reducing their harmful effects. Sensitivity of bone cells to mechanical changes suggests that modulation of fluid movement or stiffness levels within tumors could be a novel consideration in developing new treatments to prevent cancer progression.
Article
Cell Biology
Ruotian Du, Dongyan Li, Meng Zhu, Lisha Zheng, Keli Ren, Dong Han, Long Li, Jing Ji, Yubo Fan
Summary: The mechanical microenvironment and cellular senescence play a vital role in the development of primary open-angle glaucoma. This study found that shear stress has a more significant effect on normal cells compared to senescent cells. Additionally, senescent cells show reduced extracellular matrix turnover after shear stress stimulation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Gianmaria Salvio, Alessandro Ciarloni, Claudio Gianfelice, Francesca Lacche, Sofia Sabatelli, Gilberta Giacchetti, Giancarlo Balercia
Summary: Polyphenols have no significant effects on bone mineral density in postmenopausal women, but they may have a positive impact on lumbar spine BMD in studies with a duration of 24 months or longer. Additionally, polyphenols can slightly increase bone-specific alkaline phosphatase levels and decrease pyridinoline levels.
Article
Cell Biology
Zichen Xu, Keming Li, Ying Xin, Kai Tan, Mo Yang, Guixue Wang, Youhua Tan
Summary: This study reveals that fluid shear stress can eliminate the majority of suspended tumor cells and increase their nuclear size, while it has no effect on adherent tumor cells. Shear flow increases nuclear size by promoting histone acetylation, which protects suspended tumor cells from shear-induced destruction. These findings provide new insights into the mechanism of suspended tumor cells' response to shear flow and may offer new approaches for cancer cell treatment.
JOURNAL OF CELL SCIENCE
(2022)
Article
Biology
Darian Williams, Marwa Mahmoud, Renfa Liu, Aitor Andueza, Sandeep Kumar, Dong-Won Kang, Jiahui Zhang, Ian Tamargo, Nicolas Villa-Roel, Kyung-In Baek, Hwakyoung Lee, Yongjin An, Leran Zhang, Edward W. Tate, Pritha Bagchi, Jan Pohl, Laurent O. Mosnier, Eleftherios P. Diamandis, Koichiro Mihara, Morley D. Hollenberg, Zhifei Dai, Hanjoong Jo
Summary: Atherosclerosis primarily occurs in arterial regions exposed to disturbed blood flow, while regions exposed to stable flow are protected. The study discovered that KLK10, a protein sensitive to flow, is upregulated under stable flow conditions and downregulated under disturbed flow conditions. It acts as an anti-inflammatory, barrier-protective, and anti-atherogenic factor.
Article
Biochemical Research Methods
Mathias Busek, Aleksandra Aizenshtadt, Timo Koch, Anna Frank, Ludivine Delon, Mikel Amirola Martinez, Alexey Golovin, Clotilde Dumas, Justyna Stokowiec, Stefan Gruenzner, Espen Melum, Stefan Krauss
Summary: We developed a pump-less directional flow recirculating organ-on-a-chip platform that creates controlled unidirectional gravity-driven flow. The platform features two organoid compartments supported by independent perfusion channels, separated by a hydrogel barrier. We demonstrated the suitability of the platform for functional culture of endothelial cells and the viability of human stem-cell derived liver organoids, as well as the circulation of immune cells in the microfluidic channels.
Article
Endocrinology & Metabolism
Caroline Zaworski, Jonathan Cheah, Matthew F. Koff, Ryan Breighner, Bin Lin, Jonathan Harrison, Eve Donnelly, Emily M. Stein
Summary: This study evaluated texture features as a measure of bone fragility using clinically acquired MRI scans, finding that trabecular bone was more heterogeneous in fracture patients, with greater variability, disorder, and less homogeneity compared to controls.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2021)
Review
Nutrition & Dietetics
Wojciech Slupski, Paulina Jawien, Beata Nowak
Summary: Osteoporosis is a systemic bone disease characterized by reduced bone mass and deterioration of bone microarchitecture. Conventional anti-osteoporotic pharmaceutics have side effects, leading many women to seek botanicals as an alternative therapy. Plant-derived compounds are being increasingly researched as potential maintenance for bone health in perimenopausal and postmenopausal women.
Article
Medicine, General & Internal
Paolo Marzullo, Chiara Mele, Stefania Mai, Antonio Nardone, Massimo Scacchi, Gianluca Aimaretti
Summary: Postmenopausal women showed higher levels of bone turnover markers but only marginally elevated levels of sclerostin compared to premenopausal women. While there were no differences in trunk bone density between the two groups, postmenopausal women had significantly lower bone density in the hip, femoral neck, and total body.
Article
Immunology
Jennifer Barrila, Jiseon Yang, Karla P. Franco P. Melendez, Shanshan Yang, Kristina Buss, Trenton J. Davis, Bruce J. Aronow, Heather D. Bean, Richard R. Davis, Rebecca J. Forsyth, C. Mark Ott, Sandhya Gangaraju, Bianca Y. Kang, Brian Hanratty, Seth D. Nydam, Eric A. Nauman, Wei Kong, Jason Steel, Cheryl A. Nickerson
Summary: This study investigates the impact of Low Shear Modeled Microgravity (LSMMG) culture on the colonization of Salmonella Typhimurium and human intestinal diseases. The results show that LSMMG culture enhances the colonization ability of Salmonella and that the colonization ability of the Delta hfq mutant strain is higher under LSMMG conditions compared to conventional shaking cultures. In addition, infection by Salmonella induces the expression of genes related to inflammation, tissue remodeling, and wound healing in host cells. This study expands our understanding of how physical forces can affect the early stages of human enteric salmonellosis.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Entomology
Pushpalata Kayastha, Duygu Berdi, Monika Mioduchowska, Magdalena Gawlak, Aleksandra Lukasiewicz, Bartlomiej Goldyn, Lukasz Kaczmarek
Article
Entomology
Pushpalata Kayastha, Duygu Berdi, Monika Mioduchowska, Magdalena Gawlak, Aleksandra Lukasiewicz, Bartlomiej Goldyn, Szymon Jedrzejewski, Lukasz Kaczmarek
Article
Entomology
Pushpalata Kayastha, Tomasz Bartylak, Magdalena Gawlak, Lukasz Kaczmarek
Article
Zoology
Lukasz Kaczmarek, Pushpalata Kayastha, Magdalena Gawlak, Monika Mioduchowska, Milena Roszkowska
Summary: In this study, the population of Diploechiniscus oihonnae from the neotype locality in Merok, Norway was described using integrative taxonomy. No differences in chaetotaxy formula were found between life stages and sex of Dpl. oihonnae. The presence of filaments at Bd in some specimens of Dpl. oihonnae appears to be random, indicating morphological variability of the species. Genetic differences between the neotypic population and specimens from other localities were low, leading to the establishment of specimens from Merok as neotype and neoparatypes of Dpl. oihonnae, with Dpl. horningi being synonymised with Dpl. oihonnae based on morphological characters.
Article
Biodiversity Conservation
Pushpalata Kayastha, Milena Roszkowska, Monika Mioduchowska, Magdalena Gawlak, Lukasz Kaczmarek
Summary: Two new tardigrade species were described from a moss sample collected in Canada, with one belonging to Macrobiotus hufelandi complex and the other to the genus Bryodelphax. Integrative analysis using morphological and molecular data revealed significant differences between the new species and their congeners. Additionally, a new population of Mesobiotus skorackii was found in the sample, marking its first discovery outside of Kirghizia.
Article
Zoology
T. Bartylak, P. Kayastha, M. Roszkowska, A. Kepel, M. Kepel, L. Kaczmarek
Summary: This paper studied the tardigrades in samples of mosses and lichens collected from Ivohibory Forest in south-central Madagascar. A total of 39 tardigrade taxa were identified, with 21 of them being new records for Madagascar. Additionally, a new species of Bryodelphax beniowskii sp. nov. was described based on morphological analysis. The new species is most similar to Bry. olszanowskii and Bry. parvuspolaris, but differs from them mainly in terms of ventral plates arrangement and some other morphometric characters.
EUROPEAN ZOOLOGICAL JOURNAL
(2022)
Article
Biodiversity Conservation
Lukasz Kaczmarek, Pushpalata Kayastha, Milena Roszkowska, Magdalena Gawlak, Monika Mioduchowska
Summary: In this study, the integrative taxonomy approach was used to redescribe Minibiotus intermedius and a neotype was designated to stabilize its taxonomy. The genetic analyses of specimens from the neotype locality showed distinct results from previous studies. The results of this study are important for defining the taxonomy of Minibiotus.
Article
Zoology
P. Kayastha, M. Mioduchowska, M. Gawlak, L. Slugocki, R. Araujo, J. J. Goncalves Silva, L. Kaczmarek
Summary: Using integrative taxonomy, a new species, Macrobiotus kosmali sp. nov., was described from Ribeira Brava, Madeira (Portugal). Morphological and morphometric data from light microscopy and scanning electron microscopy were used to provide the species description. Additionally, genetic analysis using four DNA markers showed that the new species is most similar to four congeners, while morphologically it is most similar to several other species but differs in egg chorion and processes.
EUROPEAN ZOOLOGICAL JOURNAL
(2023)
Article
Multidisciplinary Sciences
Pushpalata Kayastha, Daniel Stec, Lukasz Slugocki, Magdalena Gawlak, Monika Mioduchowska, Lukasz Kaczmarek
Summary: A new species, Paramacrobiotus gadabouti sp. nov., was discovered and described using morphological, morphometric, and genetic data. The species belongs to the Parmacrobiotus richtersi group and exhibits richtersi-type eggs with cap-like structures at the ends. It is most similar to Pam. alekseevi, Pam. filipi, and Pam. garynahi, but differs in egg morphology and morphometrics. Unlike other species in the group, Paramacrobiotus gadabouti sp. nov. is a parthenogenetic species with a wider distribution, supporting the hypothesis of 'everything is everywhere' for tardigrades.
SCIENTIFIC REPORTS
(2023)
Article
Zoology
Pushpalata Kayastha, Piotr Rzymski, Bartlomiej Goldyn, Amit Kumar Nagwani, Edyta Fialkowska, Agnieszka Pajdak-Stos, Robert Sobkowiak, Gracjan Robotnikowski, Lukasz Kaczmarek
Summary: Perchlorates in Martian regolith pose a challenge to the survival of terrestrial life on Mars. This study assessed the activity of various invertebrates exposed to high concentrations of perchlorates and found that they were able to tolerate and recover from the exposure. These findings have implications for further research on the use of invertebrates in the development of biological systems for Mars exploration.
ZOOLOGICAL JOURNAL OF THE LINNEAN SOCIETY
(2023)
