Journal
EXPERIMENTAL PARASITOLOGY
Volume 180, Issue -, Pages 2-12Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.exppara.2017.03.003
Keywords
Chromatin; Nucleosome; ChIP; Micrococcal nuclease
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Funding
- Young Investigator Program of the Research Center of Infectious Diseases (ZINF) of the University of Wurzburg, Germany
- German Research Foundation DFG [SI 1610/2-1]
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Specific DNA-protein interactions are the basis for many important cellular mechanisms like the regulation of gene expression or replication. Knowledge about the precise genomic locations of DNA-protein interactions is important because it provides insight into the regulation of these processes. Recently, we have adapted an approach that combines micrococcal nuclease (MNase) digestion of chromatin with chromatin immunoprecipitation in Trypanosoma brucei. Here, we describe in detail how this method can be used to map the genome-wide distribution of nucleosomes or other DNA-binding proteins at high resolution in T. brucei. (C) 2017 The Authors. Published by Elsevier Inc.
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