Journal
EXPERIMENTAL CELL RESEARCH
Volume 361, Issue 2, Pages 246-256Publisher
ELSEVIER INC
DOI: 10.1016/j.yexcr.2017.10.024
Keywords
Peroxisome proliferator-activated receptor gamma; Lung adenocarcinoma; Efatutazone; Gefitinib resistance; PTEN
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Funding
- National Natural Science Foundation of China [81372396, 81570186]
- Natural Science Foundation of Jiangsu Province [bk20141017]
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Development of acquired resistance to EGFR-TKI therapy continues to be a serious clinical problem in Lung adenocarcinoma management. Peroxisome proliferator-activated receptor gamma (PPAR gamma) agonists demonstrate anti-tumor activity likely via transactivating genes that regulate cell proliferation, differentiation and apoptosis. Efatutazone, a novel later generation PPARy agonist, selectively activates PPARy target genes and has anti proliferative effects in a range of malignancies. However, the exact function and molecular mechanism of PPARy agonists efatutazone in EGFR-TKI gefitinib-resistance of Lung adenocarcinoma has not been determined. In this study, we studied the development of acquired resistance to an EGFR-TKI gefitinib in lung adenocarcinoma cells and investigated the antiproliferative effects of efatutazone in the acquired resistant cells. The treatment of gefitinib-resistant cells with efatutazone reduced the growth of gefitinib-resistant cells in a dose- and time dependent manner, and facilitated the anti-proliferative effects of gefitinib. Mechanistic investigations suggested that efatutazone acted by upregulating protein expression of PPARy, phosphatase and tensin homolog (PTEN), inactivating the Akt pathway, followed by dephosphorylation of p21Cip1 at Thr145 without affecting the transcriptional levels. Our results suggested that efatutazone, alone or in combination with gefitinib, might offer therapeutic effects in lung adenocarcinoma.
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