Article
Engineering, Biomedical
Caroline D. Hoemann, Javier Rodriguez Gonzalez, Jessica Guzman-Morales, Gaoping Chen, Ebrahim Jalali Dil, Basil D. Favis
Summary: This study found that non-inflammatory chitosan coatings promoted cartilage infiltration into and over a bioplastic scaffold, and were compatible with trabecular bone integration. Compared to drill-only defects, the 83-99-PCL scaffold induced angiogenesis and slight bone resorption, with the smallest change in bone volume fraction.
BIOACTIVE MATERIALS
(2022)
Review
Chemistry, Multidisciplinary
Hamidreza Tolabi, Niyousha Davari, Mehran Khajehmohammadi, Haniyeh Malektaj, Katayoun Nazemi, Samaneh Vahedi, Behafarid Ghalandari, Rui L. Reis, Farnaz Ghorbani, Joaquim Miguel Oliveira
Summary: Cartilage degeneration is a major cause of disability and pain worldwide, but current treatments have limited success in the long term. The convergence of tissue engineering and microfabrication principles offers advanced microfluidic technologies as alternatives. This review summarizes the developments in microfluidic hydrogel-based scaffolds for cartilage regeneration and discusses the advantages over traditional hydrogels. It also presents the technologies for fabricating microfluidic scaffolds and chips, and highlights the preclinical and clinical applications and challenges in developing microfluidic systems for cartilage repair.
ADVANCED MATERIALS
(2023)
Review
Biochemistry & Molecular Biology
Fengbo Sun, Xiaodan Sun, Hetong Wang, Chunxu Li, Yu Zhao, Jingjing Tian, Yuanhua Lin
Summary: This review systematically summarizes the research progress of 3D-printed, PLGA-based scaffolds, introduces the properties of the modified components, analyzes the influence of structure and printing method change in the printing process, illustrates the advantages and disadvantages of their applications with examples, and finally discusses the limitations and future development direction of current 3D-printed, PLGA-based materials for bone tissue repair.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biotechnology & Applied Microbiology
Marcin Szustak, Edyta Gendaszewska-Darmach
Summary: Nanocellulose is a promising biocompatible biomaterial with good mechanical properties for use as a scaffold in cartilage tissue engineering. The limited regenerative capacity of cartilage due to its lack of vascularization and sparsely distributed chondrocytes poses challenges for cell infiltration, making nanocellulose scaffolds a potential solution. The scaffolds have been shown to induce stem cell differentiation into chondrocyte phenotypes, offering opportunities for cartilage tissue development.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Polymer Science
Liang Lu, Xifu Shang, Bin Liu, Weijian Chen, Yu Zhang, Shuyun Liu, Xiang Sui, Aiyuan Wang, Quanyi Guo
Summary: This paper explores the application value of tissue engineering in vitro for the construction of artificial cartilage. By seeding healthy porcine chondrocytes on articular cartilage extracellular matrix (ACECM) scaffolds, it was found that the cells performed well in terms of adhesion and growth on the scaffolds, and they secreted collagen type II. Moreover, the porcine ACECM scaffolds showed good biocompatibility and could be used for xenotransplantation without significant immune inflammatory response. Therefore, ACECM-oriented scaffold is an ideal natural biomaterial for cartilage tissue engineering.
Article
Biochemistry & Molecular Biology
Yuan Sun, Xiaona Jia, Qing Meng
Summary: This study investigated the fabrication of nanofibrous scaffolds by blending recombinant spider silk proteins (R1SR2 and NR1SR2C) with Poly(lactic-co-glycolic) Acid (PLGA). The presence of N- and C-termini of MiSp contributed to the reduction of nanofiber diameter, increased porosity and glass transition temperatures of the scaffolds, as well as improved hydrophilicity and ultimate strain. In cell culture assays, HBMSCs grown on NR1SR2C/PLGA (20/80) scaffolds showed enhanced proliferative and adhesive abilities compared to those on pure PLGA scaffolds. These findings suggest promising applications of recombinant MiSp/PLGA blend nanofibrous scaffolds, particularly NR1SR2C/PLGA (20/80), in bone tissue engineering.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Engineering, Biomedical
Kenny A. van Kampen, Elena Olaret, Izabela-Cristina Stancu, Daniela F. Duarte Campos, Horst Fischer, Carlos Mota, Lorenzo Moroni
Summary: The main function of articular cartilage is to provide a low friction surface and protect the underlying bone. In this study, a scaffold design based on hypotrochoidal curves was developed and compared to a regular woodpile design. The hypotrochoidal design showed improved mechanical properties and enhanced extracellular matrix composition, indicating its potential for cartilage tissue engineering.
MATERIALS TODAY BIO
(2023)
Article
Engineering, Biomedical
Guillermo Bauza-Mayol, Marcos Quintela, Ava Brozovich, Michael Hopson, Shazad Shaikh, Fernando Cabrera, Aaron Shi, Federica Banche Niclot, Francesca Paradiso, Emma Combellak, Tom Jovic, Paul Rees, Ennio Tasciotti, Lewis W. Francis, Patrick Mcculloch, Francesca Taraballi
Summary: The use of biomimetic scaffolds in cartilage tissue engineering shows promising results in enhancing cartilaginous tissue formation and suppressing host cartilage degeneration. This approach may provide a clinical solution for cartilage tissue repair by modulating the immune environment to favor regenerative processes and suppress cartilage degeneration.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Polymer Science
Rachel Cordeiro, Rui D. D. Alvites, Ana C. C. Sousa, Bruna Lopes, Patricia Sousa, Ana C. C. Mauricio, Nuno Alves, Carla Moura
Summary: Osteoarthritis is a prevalent disease causing cartilage loss, and tissue engineering with different cellulose incorporated poly(epsilon-caprolactone) (PCL) scaffolds shows promise for its repair. The study manufactured and characterized PCL scaffolds with various percentages of commercial cellulose (microcrystalline and methyl cellulose) or cellulose from agro-industrial residues (corncob). The results indicate that cellulose incorporation promotes cellular adhesion/proliferation, and methyl cellulose scaffolds demonstrate compressive properties closer to native cartilaginous tissue.
Review
Chemistry, Multidisciplinary
Sandra Ruiz-Alonso, Markel Lafuente-Merchan, Jesus Ciriza, Laura Saenz-del-Burgo, Jose Luis Pedraz
Summary: Tendon injuries are a global health issue that requires complex and long-lasting rehabilitation processes. Tissue engineering, an emerging discipline, offers new approaches to tackle tendon injuries. By analyzing the nature of tendons, traditional treatments, and various tissue engineering methods, personalized regeneration with better outcomes can be achieved.
JOURNAL OF CONTROLLED RELEASE
(2021)
Review
Chemistry, Applied
Nazanin Amiryaghoubi, Marziyeh Fathi, Jaleh Barar, Yadollah Omidi
Summary: Tissue engineering involves the use of safe scaffolds, bioactive substances, and live cells to regenerate damaged tissue/organs. The success of tissue regeneration depends on the properties of the scaffold, integrity of the cells, and the biological setting. Advanced biomimetic hydrogels are promising for bone and cartilage tissue engineering due to their unique features and ability to accommodate cells and necessary substances for regeneration.
REACTIVE & FUNCTIONAL POLYMERS
(2022)
Article
Engineering, Biomedical
Xianggang Wang, Zuhao Li, Jiaqi Liu, Chenyu Wang, Haotian Bai, Xiujie Zhu, Hui Wang, Zhonghan Wang, He Liu, Jincheng Wang
Summary: This study presents the development of a bioinspired scaffold using 3D printing technology for growth plate repair. The scaffold, with surface modification and cell loading, successfully rebuilds the structure of the growth plate and reduces limb abnormalities.
MATERIALS TODAY BIO
(2023)
Article
Engineering, Biomedical
Xianggang Wang, Zuhao Li, Jiaqi Liu, Chenyu Wang, Haotian Bai, Xiujie Zhu, Hui Wang, Zhonghan Wang, He Liu, Jincheng Wang
Summary: The growth plate plays a crucial role in longitudinal bone growth, and injuries to the growth plate can result in limb abnormalities in children. This study developed a bioinspired stratified scaffold using 3D printing technology to promote the reconstruction of the growth plate structure and reduce limb abnormalities.
MATERIALS TODAY BIO
(2023)
Article
Biochemistry & Molecular Biology
Yasamin Alikhasi Amnieh, Sepideh Ghadirian, Nayereh Mohammadi, Mohammad Shadkhast, Saeed Karbasi
Summary: In this study, chitosan nanoparticles (Cs) were synthesized and incorporated into polyhydroxy butyrate (PHB) electrospun scaffolds for cartilage tissue engineering. The mechanical properties, hydrophilicity, and fiber diameter of the PHB scaffolds with varying concentrations of Cs nanoparticles were evaluated. The scaffold containing 1 wt% Cs nanoparticles (P-1Cs) showed optimal properties with increased strength and elongation at break compared to pure PHB. Crystallinity, degradation, and cell compatibility were also evaluated, and Cs nanoparticles were found to improve cell proliferation on the scaffold.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Pharmacology & Pharmacy
Thrinayan Moorthy, B. Mohamed Hathim, C. H. Murali NagaMahesh, G. Anburaj, Shiek S. S. J. Ahmed, V. Gopinath, Ganesh Munuswamy-Ramanujam, Subha Krishna Rao, Manjunath Srinivas Kamath
Summary: Bone tissue engineering (BTE) aims to treat bone abnormalities by using a porous polymer matrix that hosts cells, promotes proliferation, and releases drugs/growth factors. This study developed a 3D porous scaffold releasing kaempferol through kaempferol-entrapped albumin nanoparticles (KaeNP) and polycaprolactone (PCL). The scaffolds possessed porous structures with pore diameters essential for cell penetration and angiogenesis.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2023)
Review
Chemistry, Multidisciplinary
Tamara G. Dacoba, Shubaash Anthiya, German Berrecoso, Iago Fernandez-Marino, Carmen Fernandez-Varela, Jose Crecente-Campo, Desiree Teijeiro-Osorio, Fernando Torres Andon, Maria J. Alonso
Summary: Research in cancer therapeutics has led to the development of various pharmacological molecules, with nanotechnology playing a crucial role in overcoming biopharmaceutical challenges. Advances in drug discovery, molecular biology, and biomaterials have contributed significantly to the design of nanodelivery strategies, highlighting the potential for improved therapeutic outcomes.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Article
Instruments & Instrumentation
Hector Rilo-Alvarez, Adriana M. Ledo, Anxo Vidal, Marcos Garcia-Fuentes
Summary: TF-based therapies have great potential in medical applications, but their use as drugs depends on suitable delivery technologies. TFs can be used in protein form or encoded in polynucleotides, requiring special delivery systems for effective application. These therapies have been widely used in tissue regeneration and expanded to areas such as cancer therapy and immunomodulation.
DRUG DELIVERY AND TRANSLATIONAL RESEARCH
(2021)
Article
Engineering, Biomedical
Mario Perez Sayans, Berta Rivas Mundina, Cintia M. Chamorro Petronacci, Abel Garcia Garcia, Francisco Jose Gomez Garcia, Jose Crecente Campo, Susana Yanez Vilar, Yolanda Pineiro Redondo, Jose Rivas, Pia Lopez Jornet
Summary: This study evaluated the biological response and regenerative capacity of mesoporous silica (MS) hybrid scaffolds in different formulations, with results showing a better osteogenic response when combined with hydroxyapatite (HA).
BIO-MEDICAL MATERIALS AND ENGINEERING
(2021)
Article
Biochemistry & Molecular Biology
Ahmed El Motiam, Carlos F. de la Cruz-Herrera, Santiago Vidal, Rocio Seoane, Maite Baz-Martinez, Yanis H. Bouzaher, Emilio Lecona, Mariano Esteban, Manuel S. Rodriguez, Anxo Vidal, Manuel Collado, Carmen Rivas
Summary: The research revealed that p110 beta can be modified by SUMO1 and SUMO2, stabilizing the protein and increasing its activation of AKT, promoting cell growth and oncogenic transformation. Additionally, it was found that the regulatory subunit p85 beta counteracts the conjugation of SUMO to p110 beta, positively affecting the activation of the PI3K pathway.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Microbiology
Santiago Vidal, Maite Sanchez-Aparicio, Rocio Seoane, Ahmed El Motiam, Emily V. Nelson, Yanis H. Bouzaher, Maite Baz-Martinez, Isabel Garcia-Dorival, Susana Gonzalo, Enrique Vazquez, Anxo Vidal, Cesar Munoz-Fontela, Adolfo Garcia-Sastre, Carmen Rivas
Summary: The Ebola virus (EBOV) VP24 protein interacts with emerin, lamin A/C, and lamin B at the nuclear membrane, causing disruption and leading to abnormal nuclear morphology and cellular responses, similar to laminopathies. This interaction also impacts the expression of genes involved in DNA damage response, ERK phosphorylation, and interferon-stimulated gene induction, suggesting potential implications for EBOV replication.
Article
Multidisciplinary Sciences
Maria J. Gonzalez-Rellan, Marcos F. Fondevila, Uxia Fernandez, Amaia Rodriguez, Marta Varela-Rey, Christelle Veyrat-Durebex, Samuel Seoane, Ganeko Bernardo, Fernando Lopitz-Otsoa, David Fernandez-Ramos, Jon Bilbao, Cristina Iglesias, Eva Novoa, Cristina Ameneiro, Ana Senra, Daniel Beiroa, Juan Cunarro, Maria D. P. Chantada-Vazquez, Maria Garcia-Vence, Susana B. Bravo, Natalia Da Silva Lima, Begona Porteiro, Carmen Carneiro, Anxo Vidal, Sulay Tovar, Timo D. Muller, Johan Ferno, Diana Guallar, Miguel Fidalgo, Guadalupe Sabio, Stephan Herzig, Won Ho Yang, Jin Won Cho, Maria Luz Martinez-Chantar, Roman Perez-Fernandez, Miguel Lopez, Carlos Dieguez, Jose M. Mato, Oscar Millet, Roberto Coppari, Ashwin Woodhoo, Gema Fruhbeck, Ruben Nogueiras
Summary: The O-GlcNAcylation of p53 in the liver plays a crucial role in the physiological regulation of glucose homeostasis by controlling the expression of phosphoenolpyruvate carboxykinase.
NATURE COMMUNICATIONS
(2021)
Article
Chemistry, Multidisciplinary
Vanesa Porto, David Buceta, Blanca Dominguez, Carmen Carneiro, Erea Borrajo, Maria Fraile, Nerea Davila-Ferreira, Iria R. Arias, Jose M. Blanco, Maria C. Blanco, Juan M. Devida, Lisandro J. Giovanetti, Felix G. Requejo, Juan C. Hernandez-Garrido, Jose J. Calvino, Miguel Lopez-Haro, Giampaolo Barone, Andrew M. James, Tomas Garcia-Caballero, Diego M. Gonzalez-Castano, Martin Treder, Wolfgang Huber, Anxo Vidal, Michael P. Murphy, M. Arturo Lopez-Quintela, Fernando Dominguez
Summary: Low atomicity Ag-5 clusters exhibit catalytic and antitumor activities, suggesting potential applications in cancer therapy.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Article
Instruments & Instrumentation
German Berrecoso, Jose Crecente-Campo, Maria Jose Alonso
Summary: Nanocapsules are drug delivery nanosystems with an oily core and a polymeric shell. A liquid chromatography mass spectrometry method was developed to quantify all the components of nanocapsules. The final composition of nanocapsules was found to depend on their composition and preparation technique. Accurate quantification of nanosystems' composition is crucial for the progress of nanotechnology.
DRUG DELIVERY AND TRANSLATIONAL RESEARCH
(2022)
Article
Multidisciplinary Sciences
Manijeh Goldberg, Aaron Manzi, Amritpreet Birdi, Brandon Laporte, Peter Conway, Stefanie Cantin, Vasudha Mishra, Alka Singh, Alexander T. Pearson, Eric R. Goldberg, Sam Goldberger, Benjamin Flaum, Rifat Hasina, Nyall R. London, Gary L. Gallia, Chetan Bettegowda, Simon Young, Vlad Sandulache, James Melville, Jonathan Shum, Sonya E. O'Neill, Erkin Aydin, Alex Zhavoronkov, Anxo Vidal, Atenea Soto, Maria Jose Alonso, Ari J. Rosenberg, Mark W. Lingen, Anil D'Cruz, Nishant Agrawal, Evgeny Izumchenko
Summary: This study evaluates the use of PRV111, a nanotechnology-based system for local delivery of cisplatin, in animal models of oral cancer and in a clinical trial with OCSCC patients. The results show that PRV111 leads to increased cisplatin retention in tumors and avoids systemic toxicity. It also produces robust anti-tumor responses and complete regression of premalignant lesions. The clinical trial demonstrates efficacy and safety, with significant tumor reduction and no serious adverse events reported.
NATURE COMMUNICATIONS
(2022)
Article
Instruments & Instrumentation
Iago Fernandez-Marino, Clement Anfray, Jose Crecente-Campo, Akihiro Maeda, Aldo Ummarino, Carmen Teijeiro-Valino, Dario Blanco-Martinez, Francis Mpambani, Laurence Poul, Julie Devalliere, Matthieu Germain, Juan Correa, Marcos Fernandez-Villamarin, Paola Allavena, Eduardo Fernandez-Megia, Maria Jose Alonso, Fernando Torres Andon
Summary: This work describes the development and biological evaluation of hyaluronic acid nanocapsules (NCs) designed to target tumor-associated macrophages (TAMs). The results showed that HA nanocapsules functionalized with mannose significantly increased uptake by M2 macrophages and accumulated more in the tumor. This study provides new options for nanomedicine and immunotherapy-based cancer treatments.
DRUG DELIVERY AND TRANSLATIONAL RESEARCH
(2023)
Review
Engineering, Biomedical
Raquel Martinez-Campelo, Marcos Garcia-Fuentes
Summary: Over two decades of experience have shown that gene therapy-activated matrices are potent tools for gene modulation. mRNA-activated matrices are a recent development promising efficient and safe sustained delivery of therapeutic proteins. However, there is still limited understanding and research on the design parameters and performance of mRNA-activated matrices.
JOURNAL OF FUNCTIONAL BIOMATERIALS
(2023)
Article
Multidisciplinary Sciences
Xose Luis Dean-Ben, Justine Robin, Daniil Nozdriukhin, Ruiqing Ni, Jim Zhao, Chaim Gluck, Jeanne Droux, Juan Sendon-Lago, Zhenyue Chen, Quanyu Zhou, Bruno Weber, Susanne Wegener, Anxo Vidal, Michael Arand, Mohamad El Amki, Daniel Razansky
Summary: By using dichloromethane microdroplets with high optical absorption, the authors demonstrate 3D microangiography of the mouse brain, overcoming the issue of strong background absorption from red blood cells.
NATURE COMMUNICATIONS
(2023)
Article
Instruments & Instrumentation
German Berrecoso, Susana Belen Bravo, Iker Arriaga, Nicola Abrescia, Jose Crecente-Campo, Maria Jose Alonso
Summary: Understanding the interactions between nanocarriers and plasma proteins is crucial for controlling their biological fate. This study investigated how the surface chemical composition of polymeric nanocapsules influences their protein corona fingerprint. The results showed that the presence of polyethylene glycol (PEG) reduced protein corona, while polymeric nanocapsules containing 1,2-dioleoyl-3-trimethylammonium propane (DOTAP) exhibited high adsorption of vitronectin. By analyzing the protein corona, promising polymeric nanocapsules were identified with prolonged circulation time, enhanced tumor targeting, and preferential uptake by the immune system.
DRUG DELIVERY AND TRANSLATIONAL RESEARCH
(2023)
Article
Endocrinology & Metabolism
Edward Milbank, Nathalia R. V. Dragano, Ismael Gonzalez-Garcia, Marcos Rios Garcia, Veronica Rivas-Limeres, Liliana Perdomo, Gregory Hilairet, Francisco Ruiz-Pino, Patricia Mallegol, Donald A. Morgan, Ramon Iglesias-Rey, Cristina Contreras, Luisa Vergori, Juan Cunarro, Begona Porteiro, Aleix Gavalda-Navarro, Rebecca Oelkrug, Anxo Vidal, Juan Roa, Tomas Sobrino, Francesc Villarroya, Carlos Dieguez, Ruben Nogueiras, Cristina Garcia-Caceres, Manuel Tena-Sempere, Jens Mittag, M. Carmen Martinez, Kamal Rahmouni, Ramaroson Andriantsitohaina, Miguel Lopez
Summary: Milbank et al. demonstrate that targeted delivery of AMPK alpha 1 in SF1 neurons of the VMH using small extracellular vesicles can lead to weight loss through increased brown adipose tissue thermogenesis, independent of feeding.
Meeting Abstract
Oncology
Vanesa Porto, Carmen Carneiro, Erea Borrajo, Blanca Dominguez, J. M. Devida, L. J. Giovanetti, F. G. Requejo, G. Barone, Angela Turrero, David Buceta, Arturo Lopez, Anxo Vidal, Martin Treder, Fernando Dominguez