Article
Chemistry, Physical
Shangwei Zhang, Wei Ouyang, Xinghui Xia, Wu Wen, Lorenz Adrian, Gerrit Schueuermann
Summary: Microbial reductive dechlorination is an environmentally friendly and highly desirable method for addressing PCB pollution in soil, sediment, and underground water. The reaction is catalyzed by supernucleophilic cob(i)alamin housed in reductive dehalogenases (RDases), but the mechanism is still unknown.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2023)
Article
Engineering, Environmental
Jun Yan, Jingjing Wang, Manuel I. Villalobos Solis, Huijuan Jin, Karuna Chourey, Xiuying Li, Yi Yang, Yongchao Yin, Robert L. Hettich, Frank E. Loffler
Summary: The research demonstrates that Dehalococcoides mccartyi (Dhc) strains FL2 and 195 carrying tceA gene can grow with vinyl chloride (VC) as an electron acceptor when provided with sufficient vitamin B-12, leading to complete dechlorination of VC to ethene. Proteomic analysis shows expression of TceA in VC-grown strain FL2 cultures, and resequencing of tceA genes identified non-synonymous substitutions in both strains. The findings suggest that Dhc strains expressing TceA can respire VC, explaining ethene formation at chlorinated solvent sites where tceA dominates the RDase gene pool.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2021)
Article
Microbiology
Franziska Greiner-Haas, Martin von Bergen, Gary Sawers, Ute Lechner, Dominique Turkowsky
Summary: The strictly anaerobic bacterium Dehalococcoides mccartyi relies on organohalide respiration for energy conservation and growth. Acetylation is used by this bacterium to modulate and maintain protein levels and functionality during growth. The increase in acetylated proteins during growth may contribute to maintaining the organohalide-respiring capacity of the bacterium during the stationary phase.
Article
Microbiology
Yaru Li, He-Ping Zhao, Lizhong Zhu
Summary: The study demonstrates that FeS nanoparticles can significantly enhance the dechlorination rate of TCE and promote electron transport in D. mccartyi strain 195 by upregulating the transcriptional activity of key genes. This provides an eco-friendly and effective method for high-efficiency remediation of organohalide-polluted groundwater and soil.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Jessica M. Ewald, Jerald L. Schnoor, Timothy E. Mattes
Summary: By studying the microbial communities containing Dehalococcoides, this research reveals the differential abundance of functional genes and provides support for the bioremediation of halogenated organic contaminants.
FEMS MICROBIOLOGY ECOLOGY
(2022)
Article
Engineering, Environmental
Shangwei Zhang, Yiyang Li, Shanquan Wang
Summary: By conducting quantum chemical calculations on the microbial reductive dechlorination process of 15 PCDDs, it was found that chlorines with lower electron density are more likely to be abstracted by microbes, and that there are 105 viable and 125 unviable pathways for the transformation of 75 PCDDs. Moreover, 98% of microbial chlorine abstraction follows a specific order, and certain PCDDs can be completely dechlorinated to non-chlorinated dioxins.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Physics, Condensed Matter
J. A. Talla, E. A. Almahmoud, K. Al-Khaza'leh, H. Abu-Farsakh
Summary: In this study, the structural and electronic properties of rippled graphene and graphene with Stone-Wales defects were investigated using density functional theory. The results showed that strain and defects have significant impacts on the electronic properties of graphene.
Article
Engineering, Environmental
Shangwei Zhang, Yiyang Li, Shanquan Wang
Summary: By conducting quantum chemical calculations on the microbial reductive dechlorination process of 15 PCDDs, it was found that chlorines with lower electron density are more likely to be abstracted by microbes, and that there are 105 viable and 125 unviable pathways for the transformation of 75 PCDDs. Moreover, 98% of microbial chlorine abstraction follows a specific order, and certain PCDDs can be completely dechlorinated to non-chlorinated dioxins.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Engineering, Environmental
Rifeng Wu, Shangwei Zhang, Shanquan Wang
Summary: In this study, a new method for the remediation of polychlorinated biphenyls (PCBs) was developed by combining microbial reductive dechlorination (Bio-RD) with subsequent persulfate activation and oxidation process (PAOP). The results showed that the combination of Bio-RD and PAOP significantly improved the removal efficiency of PCBs. Different chlorinated PCBs exhibited different reactivity in the dechlorination and degradation/mineralization processes. The oxidizing species generated during the PAOP process decreased the microbial abundance, but the coexistence of other microorganisms alleviated the inhibitive effect on a specific respiratory bacterium.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Environmental Sciences
Wei-Ying Ouyang, Steffen Kuemmel, Lorenz Adrian, Yong-Guan Zhu, Hans H. Richnow
Summary: This study analyzed compound specific isotope fractionation of the antibiotic sulfamethoxazole (SMX) during anaerobic transformation using the model organism Desulfovibrio vulgaris Hildenborough. The results showed significant carbon and hydrogen isotopic fractionation due to the rearrangement of the isoxazole ring in SMX. Carbon isotope fractionation was higher than values reported for aerobic degradation or abiotic reactions, indicating its potential as a parameter to differentiate reaction mechanisms of SMX transformation. The observed apparent kinetic isotope effect for anaerobic transformation was distinct from that reported for microbial aerobic degradation. Dual-element (C-H) isotope analysis of SMX using gas chromatography was also performed in this study.
Article
Engineering, Environmental
Clara Reino, Chang Ding, Lorenz Adrian
Summary: Dehalococcoides mccartyi strain CBDB1, a strictly anaerobic organohalide-respiring bacterium, was successfully cultivated in a continuous stirring tank reactor (CSTR) using acetate, hydrogen, and the brominated amino acid D-or L-3,5-dibromotyrosine as substrates. The cultivation in the CSTR led to high cell densities and dominance of strain CBDB1, demonstrating its potential for biomass production and remediation of halogenated aromatic-contaminated environments. Proteomics analyses revealed the high expression of the reductive dehalogenase CbdbA238 with D-and L-3,5-dibromotyrosine, suggesting their promising role in biomass production.
Article
Biotechnology & Applied Microbiology
Emea Okorafor Ude, Jucelaine Haas, Mohammed Kaysar Kaiyoum, Chang Ding, Lorenz Adrian
Summary: The effect of environmental stresses on anammox bacteria was investigated using an overnight batch activity test. It was found that oxygen strongly inhibited anammox activity, but this inhibition could be reversed by sodium sulfite. Additionally, antibiotics, activated carbon, and Fe2O3 had positive effects on anammox activity.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Microbiology
Jesica M. Soder-Walz, Kenneth Wasmund, Darja Deobald, Teresa Vicent, Lorenz Adrian, Ernest Marco-Urrea
Summary: This study sequenced the genome of Dehalobacter sp. strain 8M and analyzed its protein expression. It discovered evidence of interactions among respiratory proteins and discussed implications for the respiratory architecture and advances in understanding this unique respiratory chain.
ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Engineering, Environmental
Shangwei Zhang, Wu Wen, Xinghui Xia, Wei Ouyang, Bi-xian Mai, Lorenz Adrian, Gerrit Schuurmann
Summary: Thermodynamics reveals a proton-coupled two-electron transfer mechanism in microbial B-12-dependent reductive dehalogenation of aromatics, enhancing our understanding of the dehalogenation process.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Engineering, Environmental
Rifeng Wu, Rui Shen, Zhiwei Liang, Shengzhi Zheng, Yong Yang, Qihong Lu, Lorenz Adrian, Shanquan Wang
Summary: Strategies based on ecological principles can enhance the colonization of OHRB in niches, thereby improving the bioremediation efficiency of chloroethene-contaminated sites.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Microbiology
Maria L. Bonatelli, Thore Rohwerder, Denny Popp, Yu Liu, Caglar Akay, Carolyn Schultz, Kuan-Po Liao, Chang Ding, Thorsten Reemtsma, Lorenz Adrian, Sabine Kleinsteuber
Summary: In this study, the metabolism of acesulfame by microbial degraders was investigated, leading to the identification of two gene clusters involved in the degradation pathway. Transposition of these gene clusters between chromosome and plasmid was observed. The global evolution and spread of the acesulfame degradation pathway was also elucidated, highlighting the importance of this research in understanding microbial degradation of harmful substances and environmental remediation.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Immunology
Stefan Hirschberg, Fatemeh Ghazaani, Ghada Ben Amor, Markus Pydde, Alexander Nagel, Saveria Germani, Lara Monica, Anja Schloer, Hannes Bauer, Jane Hornung, Michael Voetz, Yamen Dwai, Benjamin Scheer, Frauke Ringel, Omar Kamal-Eddin, Christoph Harms, Jonas Fuener, Lorenz Adrian, Axel Pruss, Kai Schulze-Forster, Katja Hanack, Julian Kamhieh-Milz
Summary: This study developed a cost-efficient and scalable human cell line for the production of immunogenic SARS-CoV-2 virus-like particles (VLPs). The modular design of the VLP production platform allows for rapid adaptation to new variants. The generated VLPs contained all structural proteins, exhibited specific reactivity with serum from naturally infected individuals, and demonstrated stability at low temperatures. Mice immunized with these VLPs produced neutralizing antibodies against the SARS-CoV-2 spike protein. The findings highlight the efficient, scalable, and adaptable nature of this production method, which holds therapeutic potential.
Article
Microbiology
Jimena Barrero-Canosa, Luyao Wang, Angelah Oyugi, Simon Klaes, Pascal Fischer, Lorenz Adrian, Ulrich Szewzyk, Myriel Cooper
Summary: In this study, a phage isolated from urban wastewater treatment plant effluent was characterized. The phage infects multiple strains of E. coli and has specific protein composition, affecting bacterial metabolism and stress response.
Article
Environmental Sciences
Kenneth Wasmund, Alba Trueba-Santiso, Teresa Vicent, Lorenz Adrian, Stephane Vuilleumier, Ernest Marco-Urrea
Summary: In this study, a novel DCM-degrading strain was obtained from a stable DCM-degrading consortium, and their proteome analysis revealed the significant role of mec-associated methyltransferases in anaerobic DCM metabolism.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Chemistry, Physical
Shangwei Zhang, Wei Ouyang, Xinghui Xia, Wu Wen, Lorenz Adrian, Gerrit Schueuermann
Summary: Microbial reductive dechlorination is an environmentally friendly and highly desirable method for addressing PCB pollution in soil, sediment, and underground water. The reaction is catalyzed by supernucleophilic cob(i)alamin housed in reductive dehalogenases (RDases), but the mechanism is still unknown.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2023)
Article
Microbiology
Jixing Wan, Kezhen Chen, Jingwen Chen, Zhihui Qin, Lorenz Adrian, Chaofeng Shen
Summary: Perchloroethene (PCE) is a toxic chlorinated solvent widely used around the world. Certain strains of Dehalococcoides mccartyi can transform PCE to ethene, aiding in the bioremediation of contaminated sites. In this study, the researchers observed that the presence of humic acids (HA) accelerated the dechlorination of PCE in mixed cultures containing Dehalococcoides strains. On the other hand, anthraquinone-2,6-disulfonic acid (AQDS), an analogue of HA, inhibited PCE dechlorination. The researchers concluded that HA enhanced PCE dechlorination by increasing the reaction speed between hydrogen and the dehalogenase enzyme.
FEMS MICROBIOLOGY ECOLOGY
(2022)