Article
Immunology
Dominik Noerz, Hui Ting Tang, Petra Emmerich, Katja Giersch, Nicole Fischer, Stephan Schmiedel, Marylyn M. Addo, Martin Aepfelbacher, Susanne Pfefferle, Marc Luetgehetmann
Summary: In this study, the authors developed an improved PCR test for the detection of monkeypox virus, which can be performed on widely used PCR systems. This test is crucial for timely limiting the spread of monkeypox.
EMERGING INFECTIOUS DISEASES
(2022)
Review
Infectious Diseases
Sandor Kasas, Anton Malovichko, Maria Ines Villalba, Maria Elena Vela, Osvaldo Yantorno, Ronnie G. Willaert
Summary: Rapid antibiotic susceptibility testing (AST) based on monitoring nanomotion of living organisms before and after exposure to antibiotics shows potential in fighting multidrug-resistant bacteria. This novel technique offers a quicker and more accurate way to diagnose antibiotic sensitivity, with promising applications in combating antibiotic resistance.
Article
Virology
Kosuke Kosai, Norihito Kaku, Michiko Horie, Hina Kodama, Norihiko Akamatsu, Yusuke Narita, Yasushi Matsumoto, Tetsuro Matsushita, Yohei Mizuta, Koichi Izumikawa, Hiroshi Mukae, Katsunori Yanagihara
Summary: The cobas 6800 system and cobas SARS-CoV-2 & Influenza A/B demonstrated high accuracy in detecting influenza A virus and can be valuable for clinical laboratories that need to routinely test a large number of samples.
Article
Virology
Xu Zhou, Siwen Wang, Yue Ma, Yanbing Li, Guohua Deng, Jianzhong Shi, Xiurong Wang
Summary: A reverse transcription recombinase polymerase amplification (RT-RPA)/CRISPR method was developed for rapid and sensitive detection of avian influenza virus (AIV). The new system has the potential to be an accurate, user-friendly, and inexpensive platform for point-of-care testing applications.
Article
Multidisciplinary Sciences
Xuexiang Lin, Xiao-Yu Liu, Bo Zhang, Ai-Qing Qin, Kwok-Min Hui, Kevin Shi, Yang Liu, Don Gabriel, X. James Li
Summary: A virus enzyme assay using a luciferin derivative as the substrate has been developed as an alternative method for detection of acute viral infection, particularly for influenza diagnosis. The assay showed similar detection rates for all influenza types and subtypes except for A(H7N9) due to lower viral titer. When tested with diluted specimens in comparison to a real-time polymerase chain reaction assay, the assay demonstrated high sensitivity and specificity.
SCIENTIFIC REPORTS
(2022)
Article
Biochemical Research Methods
Minju Jang, SeJin Kim, Junkyu Song, Sanghyo Kim
Summary: Respiratory illness caused by influenza virus is a serious global public health problem. This study developed a platform that combines LAMP and LFA techniques for rapid and accurate diagnosis without specialized equipment. The advantages of this platform include field usability and the ability to bypass laboratory or hospital transportation, making it beneficial for developing countries in particular.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Infectious Diseases
Nicki L. Boddington, Suzanne Elgohari, Joanna Ellis, Matthew Donati, Maria Zambon, Richard G. Pebody
Summary: The use of rapid influenza molecular testing has contributed to a significant proportion of unsubtyped influenza A hospitalizations in England. Further efforts are needed to strengthen the value of these data for public health through integrated genomic surveillance.
INFLUENZA AND OTHER RESPIRATORY VIRUSES
(2022)
Article
Pediatrics
Rana E. El Feghaly, John David Nolen, Brian R. Lee, George Abraham, Amanda Nedved, Ferdaus Hassan, Rangaraj Selvarangan
Summary: The study found that clinical suspicion for influenza is not a significant predictor for influenza RT-PCR positivity. After obtaining rapid influenza RT-PCR results, clinicians changed their original management plans in a significantly lower percentage of cases with positive results compared to cases with negative results. Implementation of rapid and accurate influenza RT-PCR is important in diagnosing influenza in children and can lead to cost savings by avoiding unnecessary antiviral prescriptions.
JOURNAL OF PEDIATRICS
(2021)
Article
Chemistry, Analytical
Muhabat Adeola Raji, Yumna Aloraij, Fatimah Alhamlan, Ghadeer Suaifan, Karina Weber, Dana Cialla-May, Juergen Popp, Mohammed Zourob
Summary: This study developed a novel, low-cost and easy-to-use colorimetric flu virus biosensor assay. The assay utilizes a sandwich format with antibodies immobilized on cotton swabs for rapid detection of flu A and B viruses.
Article
Virology
Guosong Wang, Pengfei Huang, Junping Hong, Rao Fu, Qian Wu, Ruiqi Chen, Lina Lin, Qiangyuan Han, Honglin Chen, Yixin Chen, Ningshao Xia
Summary: The ELISPOT assay based on the efficient detection of influenza virus and neutralizing antibodies is rapid, accurate, and high-throughput, serving as a powerful tool for quantification in the research and development of drugs and vaccines against influenza. The study demonstrated a good correlation with traditional PFU and PRNT methods, making it a valuable alternative in influenza studies.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Article
Chemistry, Analytical
Ji Hong Kim, Chae Hwan Cho, Jae Hwan Shin, Moon Seop Hyun, Eunha Hwang, Tae Jung Park, Jong Pil Park
Summary: An electrochemical biosensor was developed to detect the HA protein in the envelope of the influenza virus, using high-affinity peptide-displaying phage particles identified through phage display technology. The sensor showed good performance characteristics, including low limit of detection, high reproducibility, and compatibility with human plasma samples. The sensor system demonstrated advantages such as low cost, rapid analysis, and minimal sample volume requirement, making it suitable for rapid and effective detection of the influenza virus antigen.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Nanoscience & Nanotechnology
Jianyong Li, Rui Lin, Yi Yang, Rongtao Zhao, Shiping Song, Yi Zhou, Jiye Shi, Lihua Wang, Hongbin Song, Rongzhang Hao
Summary: The development of a multichannel electrochemical immunoassay platform for on-site detection of SARS-CoV-2 and A(H1N1) offers great potential for timely diagnosis and prevention of the spread of infectious diseases in communities or resource-limited regions.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Chemistry, Analytical
Vlad Shumeiko, Guy Hidas, Chen Nowogrodski, Yariv Pinto, Ofer Gofrit, Mordechai Duvdevani, Oded Shoseyov
Summary: Improper use of antibiotics leads to the increase of antibiotic-resistant bacteria, causing 700,000 deaths worldwide each year. A rapid bacterial detection device, called BactoSpin, has been developed for UTI diagnostics and antibiotic susceptibility testing (AST). The device can detect E. coli within 5 minutes and perform AST in under 2 hours, providing efficient and rapid results.
Article
Biophysics
Xingyu Ye, Haiwei Zhou, Xiang Guo, Donglai Liu, Zhonglei Li, Junwei Sun, Jun Huang, Tao Liu, Pengshu Zhao, Heshan Xu, Kai Li, Hanming Wang, Jihua Wang, Li Wang, Weili Zhao, Qian Liu, Sihong Xu, Yan Feng
Summary: In this study, a multiplex nucleic acid detection system called MULAN was developed, which integrates rapid isothermal amplification with the multiplex inclusiveness of a single Ago for simultaneous detection of multiple viruses. MULAN showed high specificity and sensitivity, and could be used for portable and visible detection.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Nanoscience & Nanotechnology
Won-Il Lee, Ashwanth Subramanian, Steffen Mueller, Kalle Levon, Chang-Yong Nam, Miriam H. Rafailovich
Summary: Rapid and accurate testing is crucial in controlling pandemic diseases like COVID-19. A new detection method based on nanoscale molecular imprints has been developed, allowing for rapid detection of viruses and proteins in bodily fluids. The method offers fast, sensitive, and specific detection, making it a highly effective point-of-care diagnostic platform.
ACS APPLIED NANO MATERIALS
(2022)
Article
Infectious Diseases
Paola Villar-Ortega, Manuela Exposito-Ruiz, Miguel Gutierrez-Soto, Miguel Ruiz-Cabello Jimenez, Jose Maria Navarro-Mari, Jose Gutierrez-Fernandez
Summary: This review examined the association between F. nucleatum and CRC. The meta-analysis results indicated a higher presence of F. nucleatum in CRC tumor tissue samples, suggesting a potential for early diagnosis.
ENFERMEDADES INFECCIOSAS Y MICROBIOLOGIA CLINICA
(2022)
Review
Medicine, General & Internal
Antonio Rosales-Castillo, Gemma Jimenez-Guerra, Lara Ruiz-Gomez, Manuela Exposito-Ruiz, Jose Maria Navarro-Mari, Jose Gutierrez-Fernandez
Summary: The detection and analysis of emerging pathogens in genitourinary infections have become increasingly important with technological advances. A systematic review and analysis of patient samples revealed that these emerging microorganisms play a significant role in genitourinary infections, with a growing resistance to conventional antibiotics. It is recommended to conduct antibiotic susceptibility testing in all cases.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Medical Laboratory Technology
Claudia Soria-Segarra, Carmen Soria-Segarra, Michelle Andrade-Soriano, Tamara Nunez Quezada, Monica C. Gestal, Jose Gutierrez-Fernandez
Summary: In low- and middle-income countries, where access to newly approved antibiotics is limited, colistin is commonly used to treat infections caused by Carbapenemase-producing Enterobacterales. This study suggests using agar dilution with a colistin concentration of 3 µg/ml as a valid method to screen for colistin resistance in order to monitor resistance levels and conduct epidemiological studies.
JOURNAL OF CLINICAL LABORATORY ANALYSIS
(2022)
Article
Genetics & Heredity
Paula Rovira, Blanca Gutierrez, Antonio Sorlozano-Puerto, Jose Gutierrez-Fernandez, Esther Molina, Margarita Rivera, Rafael Martinez-Leal, Inmaculada Ibanez-Casas, Maria Victoria Martin-Laguna, Araceli Rosa, Francisco Torres-Gonzalez, Jorge A. Cervilla
Summary: Schizophrenia risk is influenced by both Toxoplasma gondii infection and the catechol-O-methyltransferase (COMT) genotype. The risk is modified by the interaction between T. gondii infection and COMT genotype.
Article
Microbiology
Antonio Rosales-Castillo, Manuela Exposito-Ruiz, Miguel Gutierrez-Soto, Jose Maria Navarro-Mari, Jose Gutierrez-Fernandez
Summary: Microorganisms responsible for genitourinary infections now include newly emerging microbiological agents, such as Streptococcus bovis and Gardnerella spp., which are clinically relevant and of therapeutic interest. A study analyzed clinical samples from genitourinary episodes between 2016 and 2019, identifying the epidemiological characteristics, clinical presentation, antibiotic treatment, and outcomes of these infections. The most frequently detected microorganisms in urinary tract infections were S. bovis and Gardnerella spp. in females, and S. bovis, Aerococcus urinae, and Corynebacterium spp. in males. For genital infections, S. viridans was frequently detected in females, while C. glucuronolyticum and Gardnerella spp. were common in males. The study also found associations between certain microorganisms and specific patient characteristics or risk factors.
Review
Infectious Diseases
Enrique Rodriguez-Guerrero, Horacio Requena Cabello, Manuela Exposito-Ruiz, Jose Maria Navarro-Mari, Jose Gutierrez-Fernandez
Summary: This study aimed to carry out a systematic review of antibiotic resistances by urinary tract infections (UTIs) and determine changes over time in urine cultures. The study found that Enterobacteriaceae Citrobacter freundii, Enterobacter cloacae, Klebsiella aerogenes, Morganella morganii, Providencia stuartii, and Serratia marcescens (CESPM group) cause UTIs with high multiresistance rates. The study also identified antibiotics with low resistance rates for these bacteria, which can be recommended for empirical treatment of UTIs.
Article
Microbiology
Soria-Segarra Claudia, Soria-Segarra Carmen, Diaz Andres, Miranda-Ayala Marcela, Cevallos-Apolo Kerly, Bombon Moreno Bryan, Chuzan J. John, Gutierrez-Fernandez Jose
Summary: The aim of this study was to assess the risk factors for CR-CPE and describe the mortality associated with it in a low-income country. A multicenter study was conducted in Guayaquil, Ecuador, and the results showed that parenteral feeding, β-lactamase inhibitor use, recent hemodialysis, and renal failure were independent risk factors for carrying CR-CPE. Infection prevention and stewardship programs are needed to prevent the dissemination of this bacterium.
ANNALS OF CLINICAL MICROBIOLOGY AND ANTIMICROBIALS
(2023)
Article
Infectious Diseases
Luis Fernandez-Espigares, Itahisa Hernandez-Chico, Manuela Exposito-Ruiz, Antonio Rosales-Castillo, Jose Maria Navarro-Mari, Jose Gutierrez-Fernandez
Summary: This study analyzed the epidemiology and antibiotic susceptibility of significant bacteriuria and found that the adoption of EUCAST criteria did not lead to a general change in resistance rates. The results showed stable resistance profiles for most antibiotics, with exceptions for levofloxacin and nitrofurantoin. The prevalence of methicillin-resistant Staphylococcus aureus was similar in hospital and community samples. The study suggests the need to revise certain empirical criteria for Enterococcus and community-origin S. aureus.
Article
Immunology
Horacio Requena-Cabello, Enrique Rodriguez-Guerrero, Manuela Exposito-Ruiz, Jose Maria Navarro-Mari, Jose Gutierrez-Fernandez
Summary: The study aimed to review the antibiotic resistance of Pseudomonas aeruginosa and Acinetobacter baumannii from urinary tract infections between 2010 and 2021 and analyze the changes in hospital urine cultures from 2016 to 2021. The findings showed lower resistance rates for P. aeruginosa and higher resistance rates for A. baumannii compared to the systematic review. Both microorganisms exhibited reduced susceptibility to some antibiotics during the COVID-19 pandemic.
Article
Health Care Sciences & Services
Beatriz Maria Jimenez-Rodriguez, Eva Maria Trivino-Ibanez, Jose Gutierrez-Fernandez, Ana Dolores Romero-Ortiz, Eldis Maria Ramos-Urbina, Concepcion Morales-Garcia
Summary: Respiratory function deficits are common sequelae for COVID-19. This study found that certain medical conditions and genotypes may contribute to long-term lung function impairment in COVID-19 patients 12 months after infection.
Letter
Infectious Diseases
Fernando Cobo, Virginia Perez-Carrasco, Esther Gomez-Vicente, Lina Martin-Hita, Jose A. Garcia-Salcedo, Jose Maria Navarro-Maria
INFECTIOUS DISEASES NOW
(2022)
Article
Infectious Diseases
A. Franco-Acosta, B. Espadafor-Lopez, A. Rosales-Castillo, J. M. Navarro-Mari, J. Gutierrez-Fernandez
Summary: We report for the first time the association of Haemophilus pittmaniae and Haemophilus sputorum with urethritis in men who have sex with men. The susceptibility and resistance of these microorganisms were evaluated, highlighting the importance of surveillance and consideration in the differential diagnosis of genital system infections.
INFECTIOUS DISEASES NOW
(2022)
