Article
Horticulture
Lifang Geng, Lin Su, Yuan Wang, Ziwen Geng, Shang Lin, Yichang Zhang, Shuang Yu, Lufeng Fu, Qinghua Liu, Chenxia Cheng, Xinqiang Jiang
Summary: In this study, a novel dehydration response element binding protein RcTINY2 was identified and characterized in rose, which regulates the response to drought and salt stress and may serve as a candidate gene for improving plant tolerance to abiotic stress.
Article
Biochemistry & Molecular Biology
Sameer Hassan, Katrin Berk, Henrik Aronsson
Summary: Soil salinity and salt stress are major challenges in modern agriculture, with understanding plant salt tolerance mechanisms being crucial. The AP2/ERF transcription factor family, including the DREB subfamily, plays a key role in regulating salt tolerance pathways in plants. By studying the DREB family in wheat, new subfamily members were identified, confirming existing subgroups and homologous genes with Arabidopsis thaliana.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2022)
Review
Biochemistry & Molecular Biology
Xuexue Chen, Yanglin Ding, Yongqing Yang, Chunpeng Song, Baoshan Wang, Shuhua Yang, Yan Guo, Zhizhong Gong
Summary: Protein kinases play crucial roles in plant stress responses, and research progress on signal perception and transduction is essential. Future challenges include a deeper understanding of the mechanisms of action of protein kinases such as SNRKs, MAPK, CDPKs/CPKs, and RLKs in stress responses.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2021)
Article
Genetics & Heredity
Chen Dong, Qingdong Wang, Yubo Wang, Lili Qin, Yongchun Shi, Xiaoran Wang, Ran Wang
Summary: In this study, a transcription factor called NtDREB-1BL1 was found to regulate carotenoid biosynthesis by binding to the NtPSY promoter in tobacco plants. Overexpression of NtDREB-1BL1 increased carotenoid and chlorophyll contents, while RNA interference of NtDREB-1BL1 decreased these contents. Additionally, NtDREB-1BL1 overexpression enhanced drought tolerance, whereas RNA interference weakened drought tolerance. These findings highlight the importance of NtDREB-1BL1 in regulating carotenoid biosynthesis and improving drought tolerance in tobacco.
Article
Biochemistry & Molecular Biology
Jing Wang, Li Chen, Yun Long, Weina Si, Beijiu Cheng, Haiyang Jiang
Summary: The study identified a novel heat shock transcription factor gene, ZmHsf08, in maize, which negatively regulates responses to salt and drought stresses in the plant. Overexpression of ZmHsf08 resulted in increased sensitivity to salt and drought stresses, with higher ROS levels and MDA contents compared to wild-type plants. Analysis also showed that ZmHsf08 negatively regulates stress/ABA-responsive genes under salt and drought stress conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Yuqing Liang, Xiaoshuang Li, Ruirui Yang, Bei Gao, Juanxia Yao, Melvin J. Oliver, Daoyuan Zhang
Summary: The study functionally characterized a unique DREB transcription factor BaDBL1 from the desiccation tolerance moss Bryum argenteum, showing its important roles in plant responses to dehydration-rehydration, salt, cold, and abscisic acid treatments. Overexpression of BaDBL1 in Arabidopsis resulted in enhanced osmotic and salt stress tolerance by increasing anti-oxidase activities, regulating stress-related gene expression, and affecting lignin biosynthesis, making BaDBL1 a potential candidate gene for improving stress tolerance in crops.
Article
Biochemistry & Molecular Biology
Zhuo Huang, Ling Liu, Linli Jian, Wenxin Xu, Jiatong Wang, Yaxuan Li, Cai-Zhong Jiang
Summary: This study identified and characterized a dehydration-upregulated gene MfWRKY7 from a resurrection plant, which showed increased root length and tolerance to drought and salinity when overexpressed in Arabidopsis. Further investigations revealed that MfWRKY7 transgenic plants had enhanced antioxidation capacity and improved physiological traits under drought and salinity stresses.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Chongyang Li, Yalu Sun, Jian Li, Tianpeng Zhang, Fengli Zhou, Qiping Song, Yang Liu, Marian Brestic, Tony H. H. Chen, Xinghong Yang
Summary: This study investigates the different roles of ScCBF1 and StCBF1 in potato under stress conditions. The results show that ScCBF1 exhibits stronger tolerance to cold, salt, and drought stress compared to StCBF1.
JOURNAL OF PLANT PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Peng Huang, Zhengquan Ding, Min Duan, Yi Xiong, Xinxin Li, Xi Yuan, Ji Huang
Summary: In this study, a cold-induced transcription factor-LUX was identified in rice. Overexpressing OsLUX increased cold tolerance and reduced ion leakage under cold-stress conditions. Furthermore, the overexpression of OsLUX upregulated the expression levels of oxidative stress-responsive genes and improved reactive oxygen species scavenging ability.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Jinyuan Liu, Ruirui Yang, Yuqing Liang, Yan Wang, Xiaoshuang Li
Summary: Salinity is a major limiting factor in crop productivity. In this study, the researchers focused on ScDREB5, a transcription factor gene from a desiccation-tolerant moss, and found that its overexpression in Arabidopsis improved seed germination and seedling tolerance under salt stress. The overexpressing plants exhibited enhanced scavenging abilities of reactive oxygen species, upregulated stress-related gene expression, regulated ion homeostasis, and increased jasmonic acid content. These findings suggest that ScDREB5 is a promising candidate gene for crop salt stress breeding.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Chao Dong, Yue Xi, Xinlu Chen, Zong-Ming Cheng
Summary: A total of 119 AP2/EREBP genes were identified in Fragaria vesca, divided into different subfamilies. The DREB subfamily was further classified into six subgroups. Transcriptome analysis revealed distinctive expression patterns among FvDREB genes in response to drought stress, indicating their potential roles in drought tolerance of strawberry.
Article
Plant Sciences
Juanjuan Fu, Shentong Zhang, Huina Jiang, Xinfei Zhang, Hao Gao, Peizhi Yang, Tianming Hu
Summary: This study reveals that melatonin enhances cold and drought tolerance in perennial ryegrass by activating antioxidant systems and reducing H2O2 accumulation. The expression of brassinosteroids biosynthesis and signaling-related genes is regulated by melatonin. The interplay between melatonin, H2O2, and brassinosteroid signaling plays a crucial role in melatonin-induced cold/drought tolerance in perennial ryegrass.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2022)
Article
Forestry
Mingxin Yu, Junling Liu, Jing Hu, Jing Zhou, Mengjuan Zhang, Yibo Cao, Lingyun Zhang
Summary: This study revealed the functions of the PwNAC38 transcription factor in Picea wilsonii's response to drought and salt stress. PwNAC38 is involved in enhancing the scavenging efficiency of reactive oxygen species (ROS) and partially depends on the ABA signaling pathway. It plays a positive role in plant response to abiotic stress.
Review
Plant Sciences
Siarhei A. A. Dabravolski, Stanislav V. V. Isayenkov
Summary: Drought and salinity have negative effects on plant growth and productivity, but the anthocyanin biosynthesis system can activate plant stress-tolerance mechanisms and enhance drought and salt tolerance. This review focuses on the molecular and biochemical mechanisms of anthocyanins in acquired tolerance to drought and salt stresses, as well as the role of abscisic acid and abscisic-acid-miRNA156 in regulating drought-induced anthocyanin production. Additionally, it summarizes the research progress on transcription factors involved in anthocyanin biosynthesis and the development of salt and drought tolerance. Finally, the recent progress in using modern gene manipulation technologies to develop anthocyanin-enriched plants with enhanced tolerance to drought and salt stresses is discussed.
Article
Biochemistry & Molecular Biology
Longjie Ni, Zhiquan Wang, Xiangdong Liu, Shuting Wu, Jianfeng Hua, Liangqin Liu, Yunlong Yin, Huogen Li, Chunsun Gu
Summary: In this study, we analyzed the GRAS protein family in the semi mangrove plant Hibiscus hamabo and identified a gene that responds to drought and salt stress. This research provides a basis for comprehensive analysis of GRAS genes and insight into the abiotic stress response mechanism in H. hamabo.
Article
Biotechnology & Applied Microbiology
Ailing Zhang, Yang Ji, Min Sun, Chuang Lin, Puding Zhou, Juncai Ren, Dan Luo, Xiaoshan Wang, Congyu Ma, Xinquan Zhang, Guangyan Feng, Gang Nie, Linkai Huang
Summary: Pearl millet exhibits excellent drought tolerance and a quick response to drought stress within 1 hour, contributing to its overall resilience. Through physiological and transcriptomic analysis, various differentially expressed genes related to metabolic processes, MAPK signaling pathway, and plant hormone signal transduction, particularly the ABA signal transduction pathway, were identified, providing insight into the molecular mechanisms underlying drought tolerance in plants.
Article
Environmental Sciences
Gang Nie, Minyi Zhong, Jiabang Cai, Xinying Yang, Jie Zhou, Charlotte Appiah, Mingyu Tang, Xia Wang, Guangyan Feng, Linkai Huang, Xinquan Zhang
Summary: Miscanthus sinensis, as a C4 perennial grass species, shows tolerance to heavy metals such as chromium (Cr) by accumulating Cr in root cell walls and soluble fractions. Transcriptome analysis revealed the involvement of Glutathion metabolism and ABC transporters pathways in Cr tolerance of M. sinensis. These findings provide insights into the molecular mechanism of plant tolerance to heavy metal stress.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Plant Sciences
Gang Nie, Jie Zhou, Yiwei Jiang, Jie He, Yang Wang, Zongchao Liao, Charlotte Appiah, Dandan Li, Guangyan Feng, Linkai Huang, Xia Wang, Xinquan Zhang
Summary: This study investigated the transcriptomic mechanisms underlying heat tolerance mediated by exogenous MeJA in perennial ryegrass. The results showed that exogenous MeJA improved the heat tolerance by mediating the expression of genes involved in different pathways, such as chlorophyll biosynthesis and degradation, antioxidant enzyme system, HSF-HSP network, and JAs biosynthesis.
Article
Plant Sciences
Gang Nie, Zhongfu Yang, Jie He, Aiyu Liu, Jiayi Chen, Shuan Wang, Xia Wang, Guangyan Feng, Dandan Li, Yan Peng, Linkai Huang, Xinquan Zhang
Summary: The study identified 261 Miscanthus sinensis NAC genes and provided a detailed overview of the gene family, including gene structure, conserved motif compositions, chromosomal distribution, and gene duplications. Results showed variations in gene length, molecular weights, and theoretical isoelectric points of the NAC family, while gene structure and motifs were relatively conserved. Chromosomal mapping and evolutionary analysis indicated a correlation between gene duplication, especially segmental duplication, and the expansion of the M. sinensis NAC gene family.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Guangyan Feng, Pengqing Xiao, Xia Wang, Linkai Huang, Gang Nie, Zhou Li, Yan Peng, Dandan Li, Xinquan Zhang
Summary: Soil salination can lead to reduced crop production globally. This study focused on annual ryegrass and conducted a comparative transcriptome analysis to identify the core genes involved in the early responses to salt stress. The findings revealed that differences in lipid, vitamins, and carbohydrate metabolism contribute to the variation in salt tolerance between salinity-sensitive and salinity-insensitive genotypes. The study also discovered novel regulation mechanisms related to salinity stress sensing, phytohormones signaling transduction, and transcriptional regulation of the early salinity responses. Overall, this research provides insights into the mechanisms of the early salinity response in annual ryegrass and can aid in the breeding of salt-tolerant forage.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Meng Tan, Muhammad Jawad Hassan, Yan Peng, Guangyan Feng, Linkai Huang, Lin Liu, Wei Liu, Liebao Han, Zhou Li
Summary: Due to global warming and climate change, drought poses a serious threat to horticultural crop cultivation. The study found that pretreatment with spermine (Spm) significantly improved drought tolerance in creeping bentgrass. The Spm pretreatment increased the accumulation of endogenous PAs, enhanced PAs metabolism, improved GABA and nitrogen metabolism, and regulated the TCA cycle, contributing to better drought tolerance. The study provides a comprehensive understanding of PAs interaction with other metabolic pathways in regulating drought tolerance in grass species.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Samrah Afzal Awan, Imran Khan, Rezwan Tariq, Muhammad Rizwan, Xiaoshan Wang, Xinquan Zhang, Linkai Huang
Summary: This study conducted a comparative transcriptome profiling to reveal the differentially expressed transcripts (DETs) associated with salt stress in pearl millet. The results showed that salt stress significantly affected the physiological characteristics of pearl millet and upregulated and downregulated the expression of various transcripts involved in different metabolic functions. The study also identified potential roles of hormones and kinases in enhancing plant tolerance against salt stress. Moreover, various transcription factors were predicted to regulate different transcripts involved in salt stress responses. Overall, this study provides new insights into the salt stress regulation mechanisms in pearl millet and has implications for improving salinity resistance in other cereal crops.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Genetics & Heredity
Xiaowen Lu, Huan Zhang, Jialing Hu, Gang Nie, Imran Khan, Guangyan Feng, Xinquan Zhang, Xiaoshan Wang, Linkai Huang
Summary: The study conducted a comprehensive analysis of the DgbHLH family in orchardgrass, identifying 132 DgbHLH genes and classifying them into 22 subfamilies. Various analyses including gene structure, conserved motifs, chromosomal location, and gene duplication events were performed to support the classification. Transcriptome data revealed differential expression of DgbHLH genes in different tissues and under different abiotic stress conditions. Heterologous expression assays in yeast indicated that DgbHLH46 and DgbHLH128 may enhance resistance to drought and salt stress, with DgbHLH128 potentially being involved in abiotic stress response by binding to the MYC element. This study provides valuable insights for gene utilization and improvement of forage performance in orchardgrass.
FUNCTIONAL & INTEGRATIVE GENOMICS
(2022)
Article
Biochemistry & Molecular Biology
Qiuxu Liu, Fangyan Wang, Yang Shuai, Linkai Huang, Xinquan Zhang
Summary: This research utilized SMRT and NGS sequencing technologies to analyze the transcriptome of L. multiflorum, identifying numerous DEGs and alternative splicing events under drought stress, as well as obtaining a large amount of transcription factor information. The functions of R2R3-MYB gene family members were preliminarily demonstrated through analysis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Shi-Wei Xia, Lin-Feng Hang, Siyad Ali, Xiao-Yu Xu, Yan-Jun Liu, Qian-Qian Yan, Qiu-Yu Luo, Yu Li, Li-Jing Lin, Huan-Xiu Li, Xiao-Ai Zhang, Lin-Kai Huang, Xiao Ma, Yun-Song Lai
Summary: Chinese chives and garlic chives were collected and compared in terms of metabolites and genes. The study revealed that garlic chives have higher levels of amino acids and garlic odor compounds compared to Chinese chives, which is supported by the expression of biosynthetic genes. The study also identified a unique gene mutation in extra-wide-leaf garlic chives. These findings provide important insights into the strong odor of wild Chinese chives.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Genetics & Heredity
Yongjuan Jiao, Guangyan Feng, Linkai Huang, Gang Nie, Zhou Li, Yan Peng, Dandan Li, Yanli Xiong, Zhangyi Hu, Xinquan Zhang
Summary: In this study, comparative genomic analyses and phylogenetic inferences were conducted on 14 cp genomes of Dactylis glomerata from the Mediterranean and Eurasia. The results revealed that the main divergence time of D. glomerata dates back to the Miocene era, and nine highly variable regions were identified among the subspecies based on cp sequence divergence.
Article
Biochemistry & Molecular Biology
Zongchao Liao, Hossein Ghanizadeh, Xin Zhang, Hechuan Yang, Ying Zhou, Linkai Huang, Xinquan Zhang, Yiwei Jiang, Gang Nie
Summary: In this study, the miRNA expression profiles of perennial ryegrass were analyzed to uncover the regulatory mechanisms involved in heat tolerance mediated by exogenously applied methyl jasmonate (MeJA). The results showed that 102 miRNAs were up-regulated in all treatments, with different numbers of miRNAs being up-regulated in the MeJA pre-treatment, heat stress treatment, and MeJA pre-treatment + heat stress groups. Co-expression network analysis revealed that 20 miRNAs modulated 51 potential target genes, with specific pathways related to heat tolerance being influenced by MeJA. Overall, this research provides a foundation for further understanding the heat tolerance mechanism in perennial ryegrass and developing cultivars with enhanced heat tolerance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Xiaoheng Xu, Guangyan Feng, Zhongfu Yang, Qiuxu Liu, Gang Nie, Dandan Li, Ting Huang, Linkai Huang, Xinquan Zhang
Summary: In this study, transcriptome and miRNA analysis were performed to reveal the potential RNA mechanism of tiller formation under strigolactone and sucrose treatment in orchardgrass. The expression changes of specific genes and miRNA were found to be key factors in controlling tiller bud development. Strigolactones may affect the ABA biosynthesis pathway to regulate tiller bud development, and miRNA531-z may be involved in the interaction of strigolactones and sucrose in tillering regulation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Guangyan Feng, Xiaoheng Xu, Wen Liu, Feigxiang Hao, Zhongfu Yang, Gang Nie, Linkai Huang, Yan Peng, Shaun Bushman, Wei He, Xinquan Zhang
Summary: This study assessed the early-stage profiles of tiller development in orchardgrass by evaluating photosynthetic parameters, intermediate biomolecular substances, and transcriptome. Significant differences were found in photosynthetic efficiency and morphological development between high- and low-tillering genotypes. Transcriptome analysis revealed the importance of signal transduction and energy-related metabolism pathways, especially photosynthetic-related processes, in tiller induction and development. Photosynthesis, carbohydrate synthesis, nitrogen efficient utilization, and phytohormone signaling pathways were found to be closely and intrinsically linked at the transcriptional level.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agricultural Engineering
Bingchao Wu, Jie Zhu, Xixi Ma, Jiyuan Jia, Dan Luo, Qiong Ding, Xiaoshan Wang, Linkai Huang
Summary: By comparing the chloroplast genomes of upland and lowland ecotypes of switchgrass, this study reveals conservation in terms of repetitive sequences, codon usage, and the identification of a lowland-specific hotspot. The phylogenetic analysis also highlights the complexity of the evolutionary process of switchgrass.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
