Journal
MICROBIAL BIOTECHNOLOGY
Volume 9, Issue 6, Pages 824-833Publisher
WILEY-BLACKWELL
DOI: 10.1111/1751-7915.12405
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Funding
- National Science Foundation of China [21506039, 31560448]
- National Science Foundation of Guangxi [2015GXNSFBA139052]
- General Research Project of Institutions of Higher Learning of Guangxi [KY2015YB001]
- Innovation Project of Guangxi Graduate Education [YCSZ2015042]
- Teaching Related Project of the Guangxi Higher Education Excellence Program - Project of Advantageous and Characteristic Specialty (Excellent Undergraduate Major)
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Poly--glutamic acid (-PGA) is a promising microbial polymer with potential applications in industry, agriculture and medicine. The use of high -PGA-producing strains is an effective approach to improve productivity of -PGA. In this study, we developed a mutant, F3-178, from Bacillus subtilis GXA-28 using genome shuffling. The morphological characteristics of F3-178 and GXA-28 were not identical. Compared with GXA-28 (18.4 +/- 0.8gl(-1)), the yield of -PGA was 1.9-fold higher in F3-178 (34.3 +/- 1.2gl(-1)). Results from batch fermentation in 3.7l fermenter showed that F3-178 was satisfactory for industrial production of -PGA. Metabolic studies suggested that the higher -PGA yield in F3-178 could be attributed to increased intracellular flux and uptake of extracellular glutamate. Real-time PCR indicated that mRNA level of pgsB in F3-178 was 18.8-fold higher than in GXA-28, suggesting the higher yield might be related to the overexpression of genes involved in -PGA production. This study demonstrated that genome shuffling can be used for rapid improvement of -PGA strains, and the possible mechanism for the improved phenotype was also explored at the metabolic and transcriptional levels.
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