Article
Environmental Sciences
Alexander V. Sirotkin, Martina Macejkova, Adam Tarko, Zuzana Fabova, Saleh Alwasel, Abdel Halim Harrath
Summary: The study found that xylene suppresses ovarian cell functions, but buckwheat, rooibos, and vitex can mitigate these effects, acting as natural protectors against the adverse effects of xylene on ovarian cells.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Obstetrics & Gynecology
Barbora Loncova, Zuzana Fabova, Milos Mlyncek, Alexander V. Sirotkin
Summary: This study investigated the release of epiregulin (EREG) by human ovarian granulosa cells, its direct impact on basic ovarian cell functions, and its relationship with gonadotropins. The results showed a time-dependent accumulation of EREG in the medium incubated with human ovarian granulosa cells, peaking at 3 to 4 days. The addition of EREG alone increased cell viability, proliferation, and hormone release, while decreasing apoptosis. Furthermore, the gonadotropins FSH and LH enhanced the stimulatory effect of EREG on granulosa cell functions.
REPRODUCTIVE SCIENCES
(2023)
Article
Environmental Sciences
Alexander V. Sirotkin, Martina Macejkova, Adam Tarko, Zuzana Fabova, Abdulkarem Alrezaki, Saleh Alwasel, Abdel Halim Harrath
Summary: The study showed that benzene can directly suppress ovarian cell viability, progesterone, and estradiol release, while buckwheat, rooibos, and vitex can directly influence ovarian cell functions and modify the effects of benzene - preventing its toxic influence and inducing stimulatory actions on cell proliferation, apoptosis, and steroidogenesis. This indicates a potential for the use of these plant extracts in mitigating the harmful effects of benzene on ovarian cells.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Oncology
Haowei Wang, Yujia Chen, Qinzi Yuan, Lixia Chen, Peiling Dai, Xuenong Li
Summary: This study found that HRK is lowly expressed in colorectal cancer tissues. For the first time, it was shown that HRK promotes apoptosis and inhibits proliferation of colorectal cancer cells by inhibiting the PI3K/AKT/mTOR signaling pathway. HRK represents a potential target for the treatment of colorectal cancer.
FRONTIERS IN ONCOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Alexander V. Sirotkin, Martina Macejkova, Adam Tarko, Zuzana Fabova, Abdel Halim Harrath
Summary: This study examined the effects of buckwheat, rooibos, and vitex on the female reproductive system and their ability to mitigate the reproductive toxicity of toluene. The findings demonstrate that toluene has direct toxic effects on ovarian cells and that these plant extracts can either promote or mitigate the effects of toluene, with vitex potentially acting as a natural protector against toluene's suppressive effect on female reproduction.
REPRODUCTION IN DOMESTIC ANIMALS
(2023)
Article
Obstetrics & Gynecology
Zuzana Fabova, Barbora Loncova, Miroslav Bauer, Alexander Sirotkin
Summary: This study aimed to investigate the effects of miR-34a mimics and miR-34a inhibitor, both alone and in combination, on the basic functions of ovarian cells cultured with and without FSH, while also examining the impact of FSH on endogenous miR-34a expression. The findings suggest that miR-34a is involved in the control of ovarian functions and that there is a synergistic relationship between miR-34a and FSH in their actions on ovarian cell functions. Furthermore, FSH can promote miR-34a expression, and miR-34a mimics can increase the occurrence of FSHR and modify the effects of FSH on ovarian cells.
REPRODUCTIVE SCIENCES
(2023)
Article
Agriculture, Dairy & Animal Science
Z. Fabova, A. V. Sirotkin
Summary: Kisspeptin has direct effects on basic ovarian cell functions and interacts with FSH in regulating ovarian functions.
DOMESTIC ANIMAL ENDOCRINOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Deise de Lima Cardoso, Diva Anelie de Araujo Guimaraes, Pedro Mayor, Maria Auxiliadora Pantoja Ferreira, Leandro Nassar Coutinho, Frederico Ozanan Barros Monteiro
Summary: This study evaluated the developmental and functional processes of the ovaries in two Neotropical primate species, providing insights into the follicular selection, recruitment, and degeneration in these species.
Article
Cell Biology
Yanyi Xiao, Yilong Liu, Zhiwei Gao, Xian Li, Min Weng, Chenghao Shi, Cheng Wang, Linxiao Sun
Summary: Pancreatic cancer is a highly malignant tumor with increasing chemotherapeutic resistance, posing a challenge in clinical treatment. Fisetin, extracted from vegetables and fruits, shows potential as an anti-cancer agent for pancreatic cancer by targeting the PI3K/AKT/mTOR signaling cascade. Further research is needed to better understand the molecular mechanisms underlying fisetin's anti-cancer effects on pancreatic cancer.
Article
Biology
Radoslava Vlckova, Drahomira Sopkova, Zuzana Andrejcakova, Martina Lecova, Dusan Fabian, Zuzana Sefcikova, Alireza Seidavi, Alexander Sirotkin
Summary: The study showed that flaxseed and gonadotropins could mitigate the adverse effects of xylene on mouse ovaries and protect the reproductive system through changes in hormone release and reception.
Review
Biochemistry & Molecular Biology
Karolina Wolodko, Juan Castillo-Fernandez, Gavin Kelsey, Antonio Galvao
Summary: Maternal obesity can lead to impaired leptin signaling, resulting in high levels of estrogen and leptin in the bloodstream, which adversely affects ovarian function. This review highlights the potential impact of impaired leptin signaling on folliculogenesis and oocyte developmental competence in both mice and women.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Agriculture, Dairy & Animal Science
Z. Fabova, Z. Kislikova, B. Loncova, M. Bauer, A. H. Harrath, A. V. Sirotkin
Summary: This study evaluated the roles of apigenin and microRNA (miR)-152 in controlling basic ovarian granulosa cell functions and their relationships. The effects of apigenin, miR-152 analogues, and miR-152 inhibitor, alone and in combination, were examined on porcine granulosa cells. The study found that apigenin increased miR-152 expression, cell proliferation, and estradiol release, while reducing apoptosis, progesterone, and IGF-I output. MiR-152 analogues promoted cell viability, proliferation, and the release of certain hormones, while inhibiting apoptosis and estradiol output. MiR-152 inhibitor had the opposite effects. The study also demonstrated that miR-152 analogues can suppress the effects of apigenin on cell apoptosis and estradiol release.
DOMESTIC ANIMAL ENDOCRINOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Mengchi Chen, Haotian Jiang, Chunping Zhang
Summary: Primary ovarian insufficiency (POI) is a heterogeneous disease characterized by non-functional ovaries in women under 40. Genetic factors play a crucial role in POI cases, accounting for around 20% to 25%. This paper reviews various genetic causes of POI, including chromosomal abnormalities, single gene mutations, defects in mitochondrial functions, and non-coding RNA abnormalities, providing valuable insights for diagnosing and predicting POI.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Reproductive Biology
Alexander V. Sirotkin, Adam Tarko, Zuzana Fabova, Igor Valocky, Saleh Alwasel, Jan Kotwica, Abdel Halim Harrath
Summary: The aim of this in vitro study was to examine the potential effect of functional food plant extracts on mare ovarian cell functions and their response to the environmental contaminant toluene. The study found that toluene reduced cell proliferation, viability, and the release of certain hormones, but not oxytocin. Flaxseed and puncture vine extracts mitigated toluene's harmful effects, while chia extract had no effect. Toluene was able to modify the inhibitory effects of flaxseed and other plant extracts on hormone release. This study provides important insights into the effects of functional food plant extracts and toluene on mare ovarian cell functions.
Article
Endocrinology & Metabolism
Zuzana Fabova, Barbora Loncova, Miroslav Bauer, Alexander V. Sirotkin
Summary: The role of miR-125b in controlling the basic functions of porcine ovarian granulosa cells was evaluated. It was found that miR-125b can inhibit cell viability, proliferation, and hormone release, while promoting apoptosis and prostaglandin output. Therefore, miR-125b serves as an important regulator of ovarian granulosa cell functions.
GENERAL AND COMPARATIVE ENDOCRINOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
A. V. Sirotkin, V. Parkanyi, J. Pivko
Summary: The study aimed to investigate the impact of high ambient temperature on rabbit feed consumption, growth, viability, and fecundity, as well as the morphology and endocrine function of gonadal and adrenal cells. Results showed increased mortality, reduced feed consumption and growth, as well as altered hormone levels in response to high temperature exposure. These changes were attributed to decreased IGF-I and/or progesterone secretion, disruption of ovarian cell nuclei, and impaired ovarian cell response to FSH.
DOMESTIC ANIMAL ENDOCRINOLOGY
(2021)
Review
Chemistry, Medicinal
Alexander Sirotkin, Saleh Hamad Alwasel, Abdel Halim Harrath
Summary: The plant isoflavone daidzein and its metabolite equol have significant impacts on physiological processes, especially in female reproduction. They can regulate ovarian functions, cell functions, and hormone regulation, with both stimulatory and inhibitory effects on reproductive processes and diseases, suggesting potential clinical applications.
Review
Agriculture, Dairy & Animal Science
Alexander V. Sirotkin
Summary: Despite limited in vitro studies indicating potential effects of rooibos on basic ovarian cell functions and its applicability in preventing environmental contaminants on ovarian functions, further research is needed to better understand its mechanisms of action and application in reproductive biology and medicine.
JOURNAL OF ANIMAL PHYSIOLOGY AND ANIMAL NUTRITION
(2022)
Review
Chemistry, Medicinal
Alexander Sirotkin
Summary: This review summarizes the physiological effects of resveratrol on female reproductive processes and other physiological processes, indicating its diverse influences on various regulatory levels through different signaling pathways.
PHYTOTHERAPY RESEARCH
(2021)
Review
Plant Sciences
Alexander Sirotkin
Summary: Curcumin affects female reproductive processes by altering the release and reception of hormones, growth factors, and cytokines, as well as influencing ovarian cell response to these substances and external environmental factors. This suggests its potential for stimulating female reproductive processes and treating various reproductive disorders.
Article
Green & Sustainable Science & Technology
Martina Pavlikova, Alexander Sirotkin, Roman Kralik, Lucia Petrikovicova, Jose Garcia Martin
Summary: This paper outlines the adverse consequences and challenges induced by the COVID-19 pandemic for universities, using Constantine the Philosopher University in Nitra as an example. It discusses the difficulties faced in teaching and research, shares measures to minimize damages, and shows that despite complications, teaching, research, and international cooperation have continued successfully.
Article
Reproductive Biology
Barbora Loncova, Zuzana Fabova, Alexander V. Sirotkin
Summary: The study revealed that obestatin directly regulates the viability and hormone release of feline ovarian cells, with CDK and PKC potentially acting as promoters of these processes.
REPRODUCTIVE BIOLOGY
(2021)
Article
Chemistry, Medicinal
Adriana Kolesarova, Simona Baldovska, Ladislav Kohut, Alexander V. Sirotkin
Summary: This review summarizes the current knowledge on the provenance, properties, physiological and therapeutic actions of elderberry, with a focus on its effects on female reproduction. The available data suggest that elderberry has a stimulating effect on healthy ovarian cells and can reduce the viability and proliferation of ovarian cancer cells. It also affects embryogenesis and the embryonal estradiol-estradiol receptor system. The black elder constituents show potential for improving reproductive processes and treating reproductive disorders in veterinary and human medicine.
Article
Biology
Abdulkarem Alrezaki, Nouf Aldawood, Lamjed Mansour, Mukhtar Ahmed, Alexander V. Sirotkin, Saleh Alwasel, Abdel Halim Harrath
Summary: Toluene has been found to be highly toxic to humans and animals, causing damage to various tissues. However, research on its effects on ovarian function is still limited. A study on female Wistar rats showed that toluene exposure decreased ovarian weight, affected ovarian structure, and increased abnormal follicle growth. Additionally, it raised progesterone and testosterone levels and inhibited the expression of genes involved in granulosa cell proliferation and differentiation. The study also demonstrated that toluene exposure induced apoptosis and autophagy, with different effects at various doses.
Article
Reproductive Biology
Zuzana Fabova, Barbora Loncova, Milos Mlyncek, Alexander Sirotkin
Summary: The study investigated the role of kisspeptin, FSH, and their receptor in controlling basic human ovarian granulosa cell functions. It found that low dose of KP stimulated cell viability and proliferation, while high dose had the opposite effect. FSH also promoted cell functions, and there was a functional interrelationship between KP and FSH/FSHR.
REPRODUCTIVE BIOLOGY
(2022)
Article
Green & Sustainable Science & Technology
Alexander V. Sirotkin, Martina Pavlikova, Lubomir Hlad, Roman Kralik, Irina Zarnadze, Shalva Zarnadze, Lucia Petrikovicova
Summary: This paper examines the impact of the COVID-19 pandemic on international mobility, attractivity for international students, and the number of publications in two universities, CPU in Slovakia and TSMU in Georgia. The study reveals that while COVID-19 had a negative impact on international mobility, the number of international students continued to increase in both universities. The use of home offices during the pandemic had a positive effect on international publications from CPU but not TSMU. After the pandemic, international mobility increased in both universities, but did not fully return to pre-pandemic levels, and the number of publications declined.
Article
Veterinary Sciences
Andrei Balazi, Alexander Sirotkin, Pavol Makovicky, L'ubica Chrastinova, Alexander Makarevich, Peter Chrenek
Summary: The study suggests that green tea may have adverse effects on rabbit growth and sperm quality, and these effects may be dose-dependent.
VETERINARY SCIENCES
(2022)
Review
Physiology
Alexander Sirotkin, Adriana Kolesarova
Summary: Tea and coffee have the ability to promote health and prevent various disorders, partially due to the presence of molecules like caffeine. They can reduce fat storage by suppressing adipocyte functions and supporting gut microbiota, with tea also preventing obesity by reducing appetite, food consumption, and food absorption.
PHYSIOLOGICAL RESEARCH
(2021)
Article
Physiology
Alexander Sirotkin, Richard Alexa, Saleh Alwasel, Abdel Halim Harrath
Summary: Fennel stimulates proliferation and suppresses apoptosis in ovarian cells, inhibiting the effects of ghrelin. This study demonstrates the potential impact of fennel on farm animal reproduction and confirms ghrelin's role in controlling ovarian cell apoptosis and proliferation.
PHYSIOLOGICAL RESEARCH
(2021)
Article
Chemistry, Medicinal
Alexander Sirotkin, Betina Pelleova, Zuzana Fabova, Pavol Makovicky, Saleh Alwasel, Abdel Halim Harrath
Summary: This study investigates the convergence process at the market level, focusing on the role of start-ups in the probiotics field across Nutrition, Pharmaceuticals, and Cosmetics industries. It identifies probiotic products from start-ups, develops a framework for collaborations in convergence, and provides guidance on closing technology and market competence gaps through collaborations with start-ups. The study expands the literature on convergence and informs practitioners about relevant markets occupied by start-ups in the probiotic field.
