Article
Cell Biology
Robert Chevreau, Hussein Ghazale, Chantal Ripoll, Chaima Chalfouh, Quentin Delarue, Anne Laure Hemonnot-Girard, Daria Mamaeva, Helene Hirbec, Bernard Rothhut, Shalaka Wahane, Florence Evelyne Perrin, Harun Najib Noristani, Nicolas Guerout, Jean Philippe Hugnot
Summary: Ependymal cells in the adult spinal cord activate multiple signaling pathways after injury, downregulate cilia-associated genes, upregulate various genes including Osmr, and the Osmr receptor Oncostatin may regulate the fate of ependymal cells towards astrocytic differentiation by affecting cell proliferation and differentiation.
Review
Cell & Tissue Engineering
Francisco Javier Rodriguez-Jimenez, Pavla Jendelova, Slaven Erceg
Summary: Ependymal cells, dormant progenitors in the spinal cord, undergo significant changes following spinal cord injury (SCI). The understanding of molecular events that activate ependymal cells after SCI is crucial for controlling the regenerative response in damaged tissues. This review focuses on cell adhesion molecules, cellular membrane receptors, ion channels, and transcription factors that mediate SCI-induced activation of ependymal cells. The coordinated expression of receptors and ion channels regulates ependymal cell activation and may contribute to cellular replacement and tissue regeneration after SCI.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Biology
Xiaoyu Xue, Muya Shu, Zhifeng Xiao, Yannan Zhao, Xing Li, Haipeng Zhang, Yongheng Fan, Xianming Wu, Bing Chen, Bai Xu, Yaming Yang, Weiyuan Liu, Sumei Liu, Jianwu Dai
Summary: Nestin is broadly expressed in neural stem/progenitor cells during neural development, but mainly restricted to ependymal cells in adult spinal cord. Following spinal cord injury, Nestin expression is reactivated and Nestin(+) cells aggregate at the injury site. Our study indicates that Nestin(+) cells in the lesion core and edge may be derived from various cell types, with ependymal cells contributing minimally.
SCIENCE CHINA-LIFE SCIENCES
(2022)
Review
Clinical Neurology
Ruideng Wang, Rubing Zhou, Zhengyang Chen, Shan Gao, Fang Zhou
Summary: Glial cells play crucial roles in the healthy functioning of the central nervous system and in the repair of spinal cord injuries.
FRONTIERS IN NEUROLOGY
(2022)
Article
Biochemistry & Molecular Biology
Chantal Ripoll, Gaetan Poulen, Robert Chevreau, Nicolas Lonjon, Florence Vachiery-Lahaye, Luc Bauchet, Jean-Philippe Hugnot
Summary: Ependymal cells in the spinal cord play a crucial role in providing a physical barrier and circulating cerebrospinal fluid. They exhibit a dorsal-ventral expression pattern of spinal cord developmental transcription factors and express neurodevelopmental genes throughout human life. The persistence of ependymal cells and their heterogeneity highlight the importance of further investigation.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Review
Cell Biology
Lucila Perez-Gianmarco, Maria Kukley
Summary: Spinal cord injury (SCI) affects millions of people, mainly young individuals, causing deficits in sensory, motor, and autonomic functions. Regrowth of axons is a crucial goal for neurological recovery after SCI, but is challenging due to the formation of a glial scar. Traditionally considered detrimental, recent evidence suggests that glial scars are beneficial for surrounding spared tissue. Experimental studies in rodent models have shown that removal of specific glial cell populations disrupts scar organization, increases inflammation, extends tissue degeneration, and impairs recovery after SCI.
Review
Biochemistry & Molecular Biology
Gang-Un Kim, Soo-Eun Sung, Kyung-Ku Kang, Joo-Hee Choi, Sijoon Lee, Minkyoung Sung, Seung Yun Yang, Seul-Ki Kim, Young In Kim, Ju-Hyeon Lim, Min-Soo Seo, Gun Woo Lee
Summary: Spinal cord injury (SCI) is a serious condition that leads to permanent disability. Research on SCI treatment focuses on reducing neuroinflammation, with Mesenchymal stem cells (MSCs) and MSC-derived extracellular vesicles (EVs) showing potential therapeutic effects but inconsistent results in animal models. Development of novel agents for regenerative medicine is necessary.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Alejandro Torrillas de la Cal, Beatriz Paniagua-Torija, Angel Arevalo-Martin, Christopher Guy Faulkes, Antonio Jesus Jimenez, Isidre Ferrer, Eduardo Molina-Holgado, Daniel Garcia-Ovejero
Summary: In species capable of regenerating the injured spinal cord, the ependymal region plays a key role in providing new cells and coordinating regeneration. However, in the majority of humans, the central canal is lost from early childhood, being replaced by a structure with different characteristics. The study of Naked Mole-Rats and mutant hyh mice shows that they also lose the central canal during their lifetime, but the tissue substituting it is distinct from that found in humans.
Review
Cell Biology
Camila Marques de Freria, Erna Van Niekerk, Armin Blesch, Paul Lu
Summary: Spinal cord injury leads to irreversible functional impairment due to neuronal loss and disruption of connections, but neural stem cell therapy shows promising potential in promoting axonal regeneration and forming new connections. This therapy has implications for improving motor systems, including the corticospinal tract, and restoring sensory feedback in SCI patients.
Review
Immunology
Qi-Ming Pang, Si-Yu Chen, Qi-Jing Xu, Sheng-Ping Fu, Yi-Chun Yang, Wang-Hui Zou, Meng Zhang, Juan Liu, Wei-Hong Wan, Jia-Chen Peng, Tao Zhang
Summary: Inflammatory response and glial scar formation play crucial roles in spinal cord injury and repair. The role of MSCs in regulating neuroinflammation and glial scar formation after SCI is also significant.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Neurosciences
Diana G. G. Nelles, Lili-Naz Hazrati
Summary: Mild traumatic brain injury (mTBI) is a common neurological condition. Ependymal cells have potential in studying the pathogenesis of mTBI. Research has shown DNA damage and cellular senescence in ependymal cells following mTBI, as well as dysfunction in cerebrospinal fluid homeostasis. This review explores the molecular and structural alterations in ependymal cells, the potential pathological mechanisms, and possible therapeutic approaches.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2023)
Review
Emergency Medicine
Yu Zhang, Shuhai Yang, Chang Liu, Xiaoxiao Han, Xiaosong Gu, Songlin Zhou
Summary: Spinal cord injury often results in permanent disability due to the loss of functional recovery. The formation of a glial scar around the damaged tissue can interrupt the healing process, and therapeutic treatments targeting scar components are important for improving functional recovery.
Review
Orthopedics
Dayu Pan, Weixiao Liu, Shibo Zhu, Baoyou Fan, Nanxi Yu, Guangzhi Ning, Shiqing Feng
Summary: Spinal cord injury is a devastating global issue, and a profound understanding of the pathology and etiology of SCI is crucial for new therapeutic concepts. In recent years, exosomes have emerged as a promising new field for SCI treatment, with the advantage of transporting valuable materials to target cells and exerting regulatory functions. Among these, microRNAs show great potential in the pathological process and treatment of SCI through modulation of mRNA targets.
JOURNAL OF ORTHOPAEDIC TRANSLATION
(2021)
Article
Cell & Tissue Engineering
Benjamin Jevans, Nicholas D. James, Emily Burnside, Conor J. McCann, Nikhil Thapar, Elizabeth J. Bradbury, Alan J. Burns
Summary: The combination treatment of enteric neural stem cells (ENSCs) with chondroitinase ABC (ChABC) showed superior regenerative effects in repairing spinal cord injury, suggesting a potential new strategy for treating SCI.
STEM CELL RESEARCH & THERAPY
(2021)
Review
Biochemistry & Molecular Biology
Katerina Havelikova, Barbora Smejkalova, Pavla Jendelova
Summary: Spinal cord injury is a devastating medical condition that currently has no effective treatment. Stem cell treatment, whether using exogenous or endogenous stem cells, shows potential for promoting neurogenesis and aiding in recovery. This review discusses various approaches, including drug administration, reducing inflammation, neuromodulation with physical factors, and in vivo reprogramming, to enhance neurogenesis in the injured spinal cord.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Critical Care Medicine
Grzegorz Wicher, Ulrika Wallenquist, Ying Lei, Mattias Enoksson, Xiaofei Li, Barbara Fuchs, Sami Abu Hamdeh, Niklas Marklund, Lars Hillered, Gunnar Nilsson, Karin Forsberg-Nilsson
JOURNAL OF NEUROTRAUMA
(2017)
Article
Oncology
Xiaofei Li, Elisa M. Floriddia, Konstantinos Toskas, Chaima Chalfouh, Axel Honore, Anne Aumont, Nicolas Vallieres, Steve Lacroix, Karl J. L. Fernandes, Nicolas Guerout, Fanie Barnabe-Heider
EXPERIMENTAL CELL RESEARCH
(2018)
Review
Oncology
Nicolas Guerout, Xiaofei Li, Fanie Barnabe-Heider
EXPERIMENTAL CELL RESEARCH
(2014)
Article
Critical Care Medicine
Quentin Delarue, Anne Mayeur, Chaima Chalfouh, Axel Honore, Celia Duclos, Marine Di Giovanni, Xiaofei Li, Mathieu Salaun, Justine Dampierre, David Vaudry, Jean-Paul Marie, Nicolas Guerout
JOURNAL OF NEUROTRAUMA
(2020)
Article
Neurosciences
Esra Karaca, Xiaofei Li, Jakub Lewicki, Christina Neofytou, Nicolas Guerout, Fanie Barnabe-Heider, Ola Hermanson
MOLECULAR AND CELLULAR NEUROSCIENCE
(2020)
Article
Cell & Tissue Engineering
Chenhong Lin, Cinzia Calzarossa, Teresa Fernandez-Zafra, Jia Liu, Xiaofei Li, Asa Ekblad-Nordberg, Erika Vazquez-Juarez, Simone Codeluppi, Lena Holmberg, Maria Lindskog, Per Uhlen, Elisabet Akesson
STEM CELL RESEARCH & THERAPY
(2020)
Article
Clinical Neurology
C. Chalfouh, C. Guillou, J. Hardouin, Q. Delarue, X. Li, C. Duclos, D. Schapman, J-P Marie, P. Cosette, N. Guerout
Article
Clinical Neurology
Ning Xu, Tingting Xu, Raymond Mirasol, Lena Holmberg, Per Henrik Vincent, Xiaofei Li, Anna Falk, Eirikur Benedikz, Emilia Rotstein, Ake Seiger, Elisabet Akesson, Scott Falci, Erik Sundstrom
Summary: The study developed a rat model of PTS and demonstrated that transplantation of human neural stem/progenitor cells can be used as an effective treatment for PTS in an animal model closely mimicking the clinical situation. The neural cells transplanted into the cysts prevented further expansion and partially obliterated the cysts, significantly reducing their volumes.
Review
Cell & Tissue Engineering
Xiaofei Li, Erik Sundstrom
Summary: Traumatic injury of the central nervous system, such as traumatic brain injury (TBI) and spinal cord injury (SCI), results in cell loss and axonal degeneration, but limited regeneration. Stem cell therapies show promise in experimental CNS trauma, but the mechanisms behind their effects are unclear. This review discusses the pros and cons of transplantation methods and recruitment of endogenous stem cells, and proposes potential successful treatment strategies.
STEM CELLS TRANSLATIONAL MEDICINE
(2022)
Article
Medicine, General & Internal
Tingting Xu, Xiaofei Li, Yuxi Guo, Elias Uhlin, Lena Holmberg, Sumonto Mitra, Dania Winn, Anna Falk, Erik Sundstrom
Summary: This study investigates the therapeutic effects of neuroepithelial-like stem cells (NESCs) derived from Good Manufacturing Practice (GMP)-compliant induced pluripotent stem cells (iPSCs) in a rat model of post-traumatic syringomyelia (PTS). The results show that off-the-shelf NESCs transplanted 10 weeks post-injury can reduce cyst volume and stimulate tissue repair, indicating their potential as a complement to standard surgery in PTS.
Article
Cell Biology
Alexandros Sountoulidis, Sergio Marco Salas, Emelie Braun, Christophe Avenel, Joseph Bergenstrahle, Jonas Theelke, Marco Vicari, Paulo Czarnewski, Andreas Liontos, Xesus Abalo, Zaneta Andrusivova, Reza Mirzazadeh, Michaela Asp, Xiaofei Li, Lijuan Hu, Sanem Sariyar, Anna Martinez Casals, Burcu Ayoglu, Alexandra Firsova, Jakob Michaelsson, Emma Lundberg, Carolina Waehlby, Erik Sundstroem, Sten Linnarsson, Joakim Lundeberg, Mats Nilsson, Christos Samakovlis
Summary: Sountoulidis et al. have created a spatial gene expression atlas of the human embryonic lung during the first trimester of gestation, identifying 83 cell states and developmental trajectories. They integrated single-cell RNA sequencing and spatially resolved transcriptomics, providing an open platform for interactive exploration. The atlas can serve as a reference for further research and understanding of pulmonary diseases.
NATURE CELL BIOLOGY
(2023)
Article
Neurosciences
Xiaofei Li, Zaneta Andrusivova, Paulo Czarnewski, Christoffer Mattsson Langseth, Alma Andersson, Yang Liu, Daniel Gyllborg, Emelie Braun, Ludvig Larsson, Lijuan Hu, Zhanna Alekseenko, Hower Lee, Christophe Avenel, Helena Kopp Kallner, Elisabet Akesson, Igor Adameyko, Mats Nilsson, Sten Linnarsson, Joakim Lundeberg, Erik Sundstrom
Summary: The authors generated a comprehensive developmental cell atlas of the human spinal cord using single-cell and spatial multi-omics data, revealing species-specific gene regulation during development. They found that neural progenitor cell fate specification and spatial positioning were spatiotemporally regulated by specific gene sets. By integrating this atlas with pediatric ependymomas data, they identified molecular signatures and lineage-specific genes associated with cancer stem cells, providing insights into disease progression.
NATURE NEUROSCIENCE
(2023)
