Journal
ACS SENSORS
Volume 1, Issue 12, Pages 1416-1422Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acssensors.6b00493
Keywords
peptide-functionalized gold nanoparticles; colorimetric sensing; cardiac troponin I; concentration of AuNPs; size of AuNPs
Funding
- Science & Engineering Research Council (SERC) of Agency for Science, Technology and Research (A*STAR) [102 152 0015]
- government of Wenzhou, China [WIBEZD2014004-02]
- [MOE-2014-T1-001-133]
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Peptide-functionalized gold nanoparticles (AuNPs) are extensively utilized in colorimetric assays for rapid and sensitive detection of various biomedical and environmental targets. Although extensively used as colorimetric reporting systems, the role of the size and concentration of the AuNPs has not been thoroughly investigated. In this study, a 12-mer cardiac troponin I (cTnI)-specific peptide CALNN-Peg(4)-FYSHSFHENWPS was immobilized on AuNPs of different size and concentration via the CALNN anchoring sequence. A relationship was established between the total surface area of the AuNPs (binding availability) and response (centroid shift). Moreover, a colorimetric assay for cTnI operating under optimized conditions (36 nm AuNPs) yielded a limit of detection of 0.2 ng/mL (8.4 pM) when tested in diluted serum samples with an assay time of 10 min. This encouraging result opens up for further development of AuNP assays in early diagnosis of cardiac injury.
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