Journal
ACS SENSORS
Volume 1, Issue 7, Pages 934-940Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acssensors.6b00341
Keywords
gel coated disc; enzymes; biosensor; lanthanide; luminescence
Funding
- Department of Science and Technology [SR/S1/OC-68/2011, SR/NM/NS-88/2010]
- Council of Scientific and Industrial Research (CSIR)
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A paper based photoluminescent biosensor has been developed, with green Tb-luminescence as the output, for the rapid detection of beta-glucosidase and lipase, both in purified form and in biological/natural fluids. A Tb Cholate hydrogel doped with specially designed, specific enzyme substrates (pro-sensitizers) was integrated on filter paper discs. The addition of small volumes(similar to 5 mu L) of enzyme solutions on these discs led to enhanced green emission (UV lamp, lambda(ex) 365 nm), allowing qualitative detection of the enzymes-when needed, the intensity enhancement can be quantified using image processing software, or for multiple samples using a commercial plate reader. This simple technique allowed the detection of beta-glucosidase in almond extract and lipase in blood serum. Easy identification of the inhibition of beta-glucosidase was also demonstrated. The paper based sensor is inexpensive, user-friendly and needs low volumes of biological sample for analysis. This strategy has the potential to be useful for possible clinical applications.
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