Journal
GENES AND NUTRITION
Volume 11, Issue -, Pages -Publisher
BIOMED CENTRAL LTD
DOI: 10.1186/s12263-016-0521-4
Keywords
mRNA gene expression; Metabolic syndrome; PBMCs; Nordic diet; OGTT
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Funding
- NordForsk Nordic Centre of Excellence in Food, Nutrition and Health [070014]
- Akershus University College of Applied Sciences (Norway)
- Academy of Finland [131593]
- University of Oslo (Norway)
- Swedish Research council
- Svenska Diabetesforbundet
- SRP Diabetes
- Finnish Diabetes Research Foundation
- Finnish Foundation for Cardiovascular Research
- Sigrid Juselius Foundation
- Kuopio University Hospital (Finland)
- Druvan Foundation
- Skane University Hospital
- Heart-Lung Foundation
- Diabetesfonden
- Foundation Cerealia (Sweden)
- Danish Obesity Research Centre (DanORC)
- Danish Council for Strategic Research (DairyHealth, BioFunCarb) (Denmark)
- Agricultural Productivity Fund
- University of Iceland (Iceland)
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Background: Diet has a great impact on the risk of developing features of metabolic syndrome (MetS), type 2 diabetes mellitus (T2DM), and cardiovascular diseases (CVD). We evaluated whether a long-term healthy Nordic diet (ND) can modify the expression of inflammation and lipid metabolism-related genes in peripheral blood mononuclear cells (PBMCs) during a 2-h oral glucose tolerance test (OGTT) in individuals with MetS. Methods: A Nordic multicenter randomized dietary study included subjects (n = 213) with MetS, randomized to a ND group or a control diet (CD) group applying an isocaloric study protocol. In this sub-study, we included subjects (n = 89) from three Nordic centers: Kuopio (n = 26), Lund (n = 30), and Oulu (n = 33) with a maximum weight change of +/- 4 kg, high-sensitivity C-reactive protein concentration <= 10 mg L-1, and baseline body mass index <39 kg m(-2). PBMCs were isolated, and the mRNA gene expression analysis was measured by quantitative real-time polymerase chain reaction (qPCR). We analyzed the mRNA expression changes of 44 genes before and after a 2hOGTT at the beginning and the end of the intervention. Results: The healthy ND significantly down-regulated the expression of toll-like receptor 4 (TLR4), interleukin 18 (IL18), and thrombospondin receptor (CD36) mRNA transcripts and significantly up-regulated the expression of peroxisome proliferator-activated receptor delta (PPARD) mRNA transcript after the 2hOGTT compared to the CD. Conclusions: A healthy ND is able to modify the gene expression in PBMCs after a 2hOGTT. However, more studies are needed to clarify the biological and clinical relevance of these findings.
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