Journal
CHEMNANOMAT
Volume 2, Issue 5, Pages 454-460Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cnma.201600056
Keywords
cell adhesion; nanostructure; poly(N-isopropylacrylamide); surface chemistry; thioxanthone
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Funding
- JSPS KAKENHI Grant [23106009, 26350531]
- JSPS
- Cell Sheet Tissue Engineering Center (CSTEC) from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan
- Grants-in-Aid for Scientific Research [26350531] Funding Source: KAKEN
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We developed a new facile method for the preparation of temperature-responsive cell culture surfaces (TRCS) by photo-polymerization of N-isopropylacrylamide using a surface-immobilized photoinitiator. In the first step, thioxanthone groups, which served as the photoinitiator, were directly formed on polystyrene culture dish surfaces (TX-PSt). Second, poly(N-isopropylacrylamide) (PIPAAm) was grafted onto the TX-PSt surfaces (PIPAAm-TX-PSt) by using the visible light or light-emitting diode irradiation-induced surface-initiated polymerization method. By optimizing the amount of grafted PIPAAm, the resulting PIPAAm-TX-PSt successfully exhibited characteristics of a TRCS. In addition, a striped micropatterned polyacrylamide area was successively formed on the photoirradiated PIPAAm-TX-PSt surface by using a chromium photomask. These results show that temperature-responsive cell culture surfaces can be prepared through this new method.
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