Article
Engineering, Biomedical
Anthony R. Martin, Jay M. Patel, Ryan C. Locke, Michael R. Eby, Kamiel S. Saleh, Matthew D. Davidson, Mackenzie L. Sennett, Hannah M. Zlotnick, Andrew H. Chang, James L. Carey, Jason A. Burdick, Robert L. Mauck
Summary: This study developed an electrospun cell-free fibrous hyaluronic acid (HA) scaffold that delivers factors to enhance cartilage repair. The results showed that scaffolds releasing SDF resulted in an inferior cartilage healing response, highlighting the importance of appropriately screening scaffolds and therapies in large animal models.
ACTA BIOMATERIALIA
(2021)
Article
Chemistry, Multidisciplinary
Antonio Laezza, Antonietta Pepe, Brigida Bochicchio
Summary: This study successfully conjugated elastin peptide with methacrylated hyaluronic acid for the production of electrospun scaffolds. The improved processability and characteristics of the conjugated compound were confirmed through morphology and conformation studies.
CHEMISTRY-A EUROPEAN JOURNAL
(2022)
Article
Chemistry, Physical
Belen Chico, Blanca Teresa Perez-Maceda, Sara San-Jose, Maria Lorenza Escudero, Maria Cristina Garcia-Alonso, Rosa Maria Lozano
Summary: HA-functionalized CoCr surfaces exhibit better hydrophilicity and corrosion resistance, promoting the growth and adhesion of osteoblasts, making them a promising choice for bone repair implant materials.
Article
Biophysics
Yuqing Niu, Florian J. Stadler, Jiahui Fang, Massimiliano Galluzzi
Summary: This study evaluated the efficacy of hyaluronic acid functionalized tubular poly-lactic acid microfibers in directing the luminal pre-endothelialization of vascular endothelial cells. The results showed that HA/PLA microfibers had improved wettability and surface energy, leading to enhanced endothelial cell proliferation and gene expression. Overall, the biophysical cues of the scaffolds were found to be potent regulators of vascular endothelial cell endothelialization.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Article
Biochemistry & Molecular Biology
Xiaoying Zha, Xingliang Xiong, Cheng Chen, Yang Li, Lingqin Zhang, Haojiang Xie, Qifeng Jiang
Summary: This study developed a functionalized silk fibroin cutaneous scaffold incorporated with a natural medicine, usnic acid, which showed significant potential in promoting wound healing and could be a promising treatment for chronic wounds.
MACROMOLECULAR BIOSCIENCE
(2021)
Article
Pharmacology & Pharmacy
Lukasz Kaniuk, Krzysztof Berniak, Agata Lichawska-Cies, Jolanta Jura, Joanna E. Karbowniczek, Urszula Stachewicz
Summary: The enormous potential of electrospun polymer fibers in the field of biomaterials for tissue engineering and wound healing has been explored in this study. Fibrous poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) scaffolds were created to support the anchoring and proliferation of keratinocytes. Hyaluronic acid (HA) was used as a coating on PHBV fibers to improve wound closure processes.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2022)
Article
Materials Science, Textiles
Mahsa Janmohammadi, Mohammad Sadegh Nourbakhsh, Shahin Bonakdar
Summary: Skin tissue engineering is evolving with the use of electrospun scaffolds based on polycaprolactone (PCL) and hyaluronic acid (HA) containing L-ascorbic acid (AA). The combination of PCL and HA in the scaffolds enhance hydrophilicity and degradation rate, while promoting cell attachment, viability, and proliferation. The nanofibrous scaffold could potentially be applied for skin tissue engineering with improved outcomes.
FIBERS AND POLYMERS
(2021)
Article
Multidisciplinary Sciences
Panjing Yin, Weiwei Su, Ting Li, Ling Wang, Jianying Pan, Xiaoqi Wu, Yan Shao, Huabin Chen, Lin Lin, Yang Yang, Xiulin Cheng, Yanbing Li, Yaobin Wu, Chun Zeng, Wenhua Huang
Summary: This study proposed a modular hydrogel-based bioink containing microsphere-embedded chondrocytes for 3D printing multi-scale scaffolds. The gelatin methacryloyl (GelMA)/alginate microspheres were prepared using a microfluidic approach and the chondrocytes remained viable after being frozen and resuscitated. The modular hydrogel bioink could be printed using the gel-in-gel 3D bioprinting strategy to fabricate the scaffolds, which showed good cell proliferation and differentiation in vitro and biocompatibility in vivo. These findings suggest that this modular hydrogel-based bioink has potential applications in articular cartilage tissue engineering.
Article
Biochemistry & Molecular Biology
Ekaterina M. Trifanova, Maria A. Khvorostina, Aleksandra O. Mariyanats, Anastasia Sochilina, Maria E. Nikolaeva, Evgeny Khaydukov, Roman A. Akasov, Vladimir K. Popov
Summary: Modern biocompatible materials and advanced techniques are used to fabricate tissue engineering constructs (TECs) for the replacement of body defects and tissue regeneration. The TECs, made from synthetic and natural polymers, are impregnated with UCNP nanoparticles for controlling the tissue/scaffold interaction. The UCNP-impregnated scaffolds demonstrate high efficiency for cell attachment, proliferation, and colonization, and can be visualized and monitored in real-time.
Article
Biology
Ekaterina M. M. Trifanova, Gulalek Babayeva, Maria A. A. Khvorostina, Aleksandra V. V. Atanova, Maria E. E. Nikolaeva, Anastasia V. V. Sochilina, Evgeny V. V. Khaydukov, Vladimir K. K. Popov
Summary: Non-invasive visualization and monitoring of tissue-engineered structures in a living organism is a challenge. We used upconversion nanoparticles (UCNPs) as photoluminescent nanomarkers in scaffolds to solve this problem. The results showed that the UCNPs' photoluminescent signal decreased gradually in all the examined scaffolds, indicating their biodegradation and release of photoluminescent nanoparticles into the surrounding tissues.
Article
Biochemistry & Molecular Biology
Patricia Galvez-Martin, Cristina Soto-Fernandez, Jessica Romero-Rueda, Jesus Cabanas, Anna Torrent, Gloria Castells, Daniel Martinez-Puig
Summary: The objective of this study was to extract, characterise and evaluate the potential of a hyaluronic acid (HA) matrix ingredient for anti-aging. The results showed that the HA matrix had regenerative, moisturising, anti-aging and antioxidant effects on skin cells, as well as potential oral and topical applications for skin care products.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Polymer Science
Sena Su, Tuba Bedir, Cevriye Kalkandelen, Ahmet Ozan Basar, Hilal Turkoglu Sasmazel, Cem Bulent Ustundag, Mustafa Sengor, Oguzhan Gunduz
Summary: New composite materials based on poly(epsilon-caprolactone)/polyethylene oxide loaded with hyaluronic acid (HA) and keratin (KR) were produced using emulsion and coaxial electrospinning methods. The fibers demonstrated distinct morphological and chemical characteristics, and showed potential for wound healing applications through in vitro studies, as the HA and KR incorporated into the fiber structure increased cell viability and proliferation synergistically.
EUROPEAN POLYMER JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Francesco Bellia, Valeria Lanza, Irina Naletova, Barbara Tomasello, Valeria Ciaffaglione, Valentina Greco, Sebastiano Sciuto, Pietro Amico, Rosanna Inturri, Susanna Vaccaro, Tiziana Campagna, Francesco Attanasio, Giovanni Tabbi, Enrico Rizzarelli
Summary: A series of copper(II) complexes varying the molecular weight of Hyaluronic acid (Hy, x = 200 or 700 kDa) conjugated with carnosine (Car) were synthesized and characterized. The metal complexes showed excellent Cu, Zn-superoxide dismutase (SOD1) mimicking activity. The presence of non-functionalized Hyaluronic acid OH groups enhanced the scavenger activity of the copper(II) complexes.
Review
Engineering, Biomedical
Niranjan G. Kotla, Isma Liza Mohd Isa, Aitor Larranaga, Balaji Maddiboyina, Samantha K. Swamy, Gandhi Sivaraman, Praveen K. Vemula
Summary: In recent years, there has been a significant expansion in the research and clinical applications of hyaluronic acid-based scaffolds, medical devices, and bioconjugate systems. These developments are driven by the abundance of hyaluronic acid in mammalian tissues and its potential for chemical modifications. This review summarizes the importance of chemical modifications of hyaluronic acid, various advancements in bioconjugate derivatives, and their potential advantages in terms of physicochemical and pharmacological properties.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Ugo D'Amora, Alessandra Soriente, Alfredo Ronca, Stefania Scialla, Martina Perrella, Paola Manini, Jun Wei Phua, Christoph Ottenheim, Rocco Di Girolamo, Alessandro Pezzella, Maria Grazia Raucci, Luigi Ambrosio
Summary: An optimized extraction protocol for eumelanins from black soldier flies was used to study natural eumelanin pigments and develop sustainable scaffolds. Based on methacrylated hyaluronic acid and BSF-Eumel, 3D printed scaffolds exhibited favorable physico-chemical, mechanical, and biological properties to support the growth and differentiation of human mesenchymal stem cells.
Article
Chemistry, Multidisciplinary
Yazhong Bu, Wenwen Zhang, Sergio Martin-Saldana, Amir M. Alsharabasy, Mark Da Costa, Luyao Feng, Ze Zhang, Xinlan Ge, Chonghui Li, Shichun Lu, Abhay Pandit
Summary: This study develops a series of multifunctional bioadhesives using succinic anhydride-modified hyaluronic acid (HASA) and plant-derived tannic acid (TA) with centrifuging and freeze-drying. By varying the ratio of HASA to TA, HASATA 1:2, 1:7, and 1:14 are fabricated with tunable adhesive strength. The bioadhesives accelerate wound healing, exhibit antioxidant properties, and have antibacterial activity, making them suitable for noninvasive wound closure in the clinical setting.
ADVANCED MATERIALS INTERFACES
(2023)
Review
Chemistry, Multidisciplinary
Sean O'Halloran, Abhay Pandit, Andreas Heise, Andrew Kellett
Summary: Two-photon polymerization (TPP) is a leading method for microscale fabrication of customized polymeric devices and surfaces. It has a wide range of applications in optics, drug delivery, tissue engineering, and microfluidics. TPP has evolved from standard acrylate- and epoxy-based photoresists to custom formulated monomers that can modify the properties of resulting structures. This article provides an overview of the essential principles and advanced chemical modification strategies for TPP materials and introduces commonly used photoresists and their applications.
Article
Clinical Neurology
Rosanna Rossi, Andrew Douglas, Sara Molina Gil, Duaa Jabrah, Abhay Pandit, Michael Gilvarry, Ray McCarthy, James Prendergast, Katarina Jood, Petra Redfors, Annika Nordanstig, Erik Ceder, Dennis Dunker, Jeanette Carlqvist, Istvan Szikora, John Thornton, Georgios Tsivgoulis, Klearchos Psychogios, Turgut Tatlisumak, Alexandros Rentzos, Karen M. M. Doyle
Summary: By analyzing 122 thrombi from 80 patients with acute ischemic stroke, it was found that elevated S100b levels were associated with an increased risk of PTIH. Additionally, higher S100b expression, higher NIHSS scores at admission, and a greater number of passes during thrombectomy were all associated with PTIH.
FRONTIERS IN NEUROLOGY
(2023)
Article
Multidisciplinary Sciences
Paolo Contessotto, Renza Spelat, Federico Ferro, Vaidas Vysockas, Ausra Krivickiene, Chunsheng Jin, Sandrine Chantepie, Clizia Chinello, Audrys G. Pauza, Camilla Valente, Mindaugas Rackauskas, Alvise Casara, Vilma Zigmantaite, Fulvio Magni, Dulce Papy-Garcia, Niclas G. Karlsson, Egle Ereminiene, Abhay Pandit, Mark Da Costa
Summary: The lack of a reproducible pre-clinical model hinders the study of the pathophysiology and possible interventions for non-ST-segment elevation myocardial infarction (NSTEMI). In this study, authors develop an ovine model to reproduce post-ischemic remodeling in NSTEMI and reveal distinct complex sugar moieties in cellular membranes and extracellular matrix patterns in infarcted tissue.
NATURE COMMUNICATIONS
(2023)
Article
Engineering, Biomedical
Eugenia Pugliese, Ignacio Sallent, Sofia Ribeiro, Alexandre Trotier, Stefanie H. Korntner, Yves Bayon, Dimitrios I. Zeugolis
Summary: A three-layer scaffold was designed to mimic the matrix composition of the enthesis and loaded with bioactive molecules to promote cell differentiation. The cells differentiated towards osteogenic and fibrocartilage lineages in response to the scaffold composition. Compartmentalised scaffolds show potential for repairing and regenerating the enthesis.
MATERIALS TODAY BIO
(2023)
Review
Biology
Caglar Ersanli, Athina Tzora, Chrysoula (Chrysa) Voidarou, Stylianos Skoufos, Dimitrios I. Zeugolis, Ioannis Skoufos
Summary: Cutaneous wound healing is a complex process that can be affected by the penetration of microorganisms, leading to imbalances in the skin microbiota. Understanding the major bacteria species in wounds and their interaction with the immune system is crucial for wound healing.
Article
Engineering, Biomedical
Caglar Ersanli, Chrysoula (Chrysa) Voidarou, Athina Tzora, Konstantina Fotou, Dimitrios I. Zeugolis, Ioannis Skoufos
Summary: This article briefly reviews preclinical studies on electrospun nanofibrous scaffolds as delivery systems for herbal extracts, with a particular focus on wound-healing applications in the past five years. The indirect effects of herbal extracts on wound healing by altering the characteristics of electrospun mats are also discussed.
JOURNAL OF FUNCTIONAL BIOMATERIALS
(2023)
Article
Chemistry, Medicinal
Amir M. Alsharabasy, Amal Aljaabary, Raghvendra Bohara, Pau Farras, Sharon A. Glynn, Abhay Pandit
Summary: The study demonstrates that hemin can be used as a potential anticancer agent by scavenging nitric oxide (NO), inhibiting cancer cell migration, and regulating the expression of migration-associated proteins.
ACS PHARMACOLOGY & TRANSLATIONAL SCIENCE
(2023)
Article
Materials Science, Biomaterials
Mangesh Morey, Aitor Larranaga, Sunny Akogwu Abbah, Raghvendra Bohara, Amal Aljaabary, Abhay Pandit
Summary: Nucleic acid therapy has the potential to promote wound healing through gene expression modification, but protecting the nucleic acid payload and achieving efficient bioresponsive delivery remain challenging. In this study, a glucose-responsive gene delivery system was designed using fibrin-coated polymeric microcapsules, which effectively loaded and released multiple nucleic acids with no cytotoxic effects in vitro. When applied to diabetic wounds, the system improved wound healing and upregulated key proteins involved in the process. This hydrogel-based system shows promise for promoting wound healing and can be encapsulated with different therapeutic nucleic acids.
Meeting Abstract
Cell & Tissue Engineering
Honorata Kraskiewicz, Piotr Hinc, Agnieszka Krawczenko, Aleksandra Bielawska-Pohl, Maria Paprocka, Danuta Witkowska, Abhay Pandit, Aleksandra Klimczak
TISSUE ENGINEERING PART A
(2023)
Meeting Abstract
Cell & Tissue Engineering
Jack Schofield, Thomas Ritter, Abhay Pandit
TISSUE ENGINEERING PART A
(2023)
Review
Medicine, General & Internal
Wang-Lin Duan, Li-Ning Zhang, Raghvendra Bohara, Sergio Martin-Saldana, Fei Yang, Yi-Yang Zhao, Yong Xie, Ya-Zhong Bu, Abhay Pandit
Summary: This review summarizes the advances in using adhesive hydrogel for osteoarthritis (OA) treatment, including commonly used hydrogels with their adhesion mechanisms and components, as well as bioactive therapeutic strategies. By presenting an interdisciplinary perspective, this review aims to provide a comprehensive insight for designing novel bioadhesive systems for OA therapy.
MILITARY MEDICAL RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Enrico Bagnoli, Alexandre Trotier, Jill Mcmahon, Leo R. Quinlan, Manus Biggs, Abhay Pandit, Una Fitzgerald
Summary: Parkinson's disease is a progressive neurodegenerative disorder with unknown etiology and no cure. The olfactory bulb, responsible for processing olfactory information, is affected early in the disease. Research has shown that exposure to the dopamine metabolite DOPAL can replicate many aspects of Parkinson's disease pathology.
Article
Biochemistry & Molecular Biology
Enrico Bagnoli, Alexandre Trotier, Jill McMahon, Leo R. Quinlan, Manus Biggs, Abhay Pandit, Una Fitzgerald
Summary: Parkinson's disease is a progressive neurodegenerative disorder with unknown etiology and no cure. The olfactory bulb, involved in processing olfactory information, is affected early in the disease. The study demonstrates that dopamine metabolite DOPAL can replicate Parkinson's pathology, shedding light on the disease mechanism.
Review
Medicine, Research & Experimental
Catherine R. Healy, Georgina Gethin, Abhay Pandit, David P. Finn
Summary: Chronic wounds impose a significant burden on individuals and the healthcare system, with pain being the most challenging aspect of living with these wounds. The cutaneous endocannabinoid system shows potential in addressing wound-related pain and inflammation, as well as promoting wound healing. However, more research, including preclinical studies and randomized control trials, is needed to fully explore the therapeutic applications of the endocannabinoid system in chronic wound management.
BIOMEDICINE & PHARMACOTHERAPY
(2023)