Journal
FRONTIERS IN IMMUNOLOGY
Volume 7, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fimmu.2016.00512
Keywords
systemic lupus erythematosus; CD16(+) monocytes; expansion; T cell activation; B cell response
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Funding
- National Natural Science Foundation of China [81172844, 81471600, 81671609, 81671604, 81302554]
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Background: The roles that CD16(+) monocyte subset plays in T-cell activation and B-cell response have not been well studied in systemic lupus erythematosus (SLE). Objective: The present study aimed to investigate the distribution of CD16(+) monocyte subsets in SLE and explore their possible roles in T-cell activation and B-cell differentiation. Methods: The frequencies of monocyte subsets in the peripheral blood of healthy controls (HCs) and patients with SLE were determined by flow cytometry. Monocyte subsets were sorted and cocultured with CD4(+) T cells and CD19(+) B cells. Then, T and B cells were collected for different subset detection, while the supernatants were collected for immunoglobulin G, IgA, and IgM or interferon-. and interleukin-17A detection by enzyme-linked immunosorbent assay. Results: Our results showed that CD16(+) monocytes exhibited a proinflammatory phenotype with elevated CD80, CD86, HLA-DR, and CX3CR1 expression on the cell surface. It's further demonstrated that CD16(+) monocytes from patients and HCs shared different cell-surface marker profiles. The CD16(+) subset was enriched in SLE and had an exacerbated capacity to promote CD4(+) T cell polarization into a Th17 phenotype. Also, CD16(+) monocytes had enhanced impacts on CD19(+) B cells to differentiate into plasma B cells and regulatory B cells with more Ig production. Conclusion: This study demonstrated that CD16(+) monocytes, characterized by different cell-surface marker profiles, were enriched and played a critical role in driving the pathogenic T-and B-cell responses in patients with SLE.
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