Journal
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY
Volume 72, Issue -, Pages 520-523Publisher
INT UNION CRYSTALLOGRAPHY
DOI: 10.1107/S2059798316001480
Keywords
serial femtosecond crystallography; X-ray free-electron laser; liquid-droplet injector; lysozyme
Funding
- X-ray Free-Electron Laser Priority Strategy Program (MEXT)
- JSPS KAKENHI [15K05407]
- Grants-in-Aid for Scientific Research [15K05407] Funding Source: KAKEN
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A liquid-droplet injector has been developed that delivers pristine microcrystals to an X-ray irradiation area for conducting serial femtosecond crystallography (SFX) with an X-ray free-electron laser (XFEL). By finely tuning the pulsed liquid droplets in time and space, a high hit rate of the XFEL pulses to microcrystals in the droplets was achieved for measurements using 5 mm tetragonal lysozyme crystals, which produced 4265 indexable diffraction images in about 30 min. The structure was determined at a resolution of 2.3 angstrom from < 0.3 mg of protein. With further improvements such as reduction of the droplet size, liquid droplets have considerable potential as a crystal carrier for SFX with low sample consumption.
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