Journal
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY
Volume 72, Issue -, Pages 1090-1099Publisher
INT UNION CRYSTALLOGRAPHY
DOI: 10.1107/S2059798316012833
Keywords
BioSAXS; ion-exchange chromatography; online purification
Funding
- ANR grant (REPLIPOX) [ANR-13-BSV8-0014]
- Agence Nationale de la Recherche (ANR) [ANR-13-BSV8-0014] Funding Source: Agence Nationale de la Recherche (ANR)
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Biological small-angle X-ray scattering (BioSAXS) is a powerful technique to determine the solution structure, particle size, shape and surface-to-volume ratio of macromolecules. However, a drawback is that the sample needs to be monodisperse. To ensure this, size-exclusion chromatography (SEC) has been implemented on many BioSAXS beamlines. Here, the integration of ion-exchange chromatography (IEC) using both continuous linear and step gradients on a beamline is described. Background subtraction for continuous gradients by shifting a reference measurement and two different approaches for step gradients, which are based on interpolating between two background measurements, are discussed. The results presented here serve as a proof of principle for online IEC and subsequent data treatment.
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