Protein fusion tags for efficient expression and purification of recombinant proteins in the periplasmic space of E. coli

Positional effects of fusion partners on the yield and solubility of MBP fusion proteins

(2015) Sreejith Raran-Kurussi et al.   PROTEIN EXPRESSION AND PURIFICATION

Biopharmaceutical benchmarks 2014

(2014) Gary Walsh   NATURE BIOTECHNOLOGY

Fusion tags for protein solubility, purification and immunogenicity in Escherichia coli: the novel Fh8 system

(2014) Sofia Costa et al.   Frontiers in Microbiology

Recombinant protein expression in Escherichia coli: advances and challenges

(2014) Germán L. Rosano et al.   Frontiers in Microbiology

Expression in Escherichia coli: becoming faster and more complex

(2013) Renaud Vincentelli et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Production of prone-to-aggregate proteins

(2013) Mario Lebendiker et al.   FEBS LETTERS

Protein A-Mouse Acidic Mammalian Chitinase-V5-His Expressed in Periplasmic Space of Escherichia coli Possesses Chitinase Functions Comparable to CHO-Expressed Protein

(2013) Akinori Kashimura et al.   PLoS One

High yield purification of nanobodies from the periplasm of E. coli as fusions with the maltose binding protein

(2013) Valencio Salema et al.   PROTEIN EXPRESSION AND PURIFICATION

Strategies for the Production of Recombinant Protein in Escherichia coli

(2013) Gopal Jee Gopal et al.   PROTEIN JOURNAL

Protein refolding using chemical refolding additives

(2012) Satoshi Yamaguchi et al.   Biotechnology Journal

The Ability to Enhance the Solubility of Its Fusion Partners Is an Intrinsic Property of Maltose-Binding Protein but Their Folding Is Either Spontaneous or Chaperone-Mediated

(2012) Sreejith Raran-Kurussi et al.   PLoS One

Refolding of proteins from inclusion bodies: rational design and recipes

(2011) Anindya Basu et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Special issue on the production of recombinant proteins

(2011) Sergio Sanchez et al.   BIOTECHNOLOGY ADVANCES

Overexpression and purification of U24 from human herpesvirus type-6 in E. coli: unconventional use of oxidizing environments with a maltose binding protein-hexahistine dual tag to enhance membrane protein yield

(2011) Andrew R Tait et al.   Microbial Cell Factories

Thermodynamics of maltose binding protein unfolding

(2010) Valery Novokhatny et al.   PROTEIN SCIENCE

Suppression of Protein Aggregation by L-Arginine

(2009) Christian Lange et al.   CURRENT PHARMACEUTICAL BIOTECHNOLOGY

A novel Ecotin-Ubiquitin-Tag (ECUT) for efficient, soluble peptide production in the periplasm of Escherichia coli

(2009) Michael Paal et al.   Microbial Cell Factories