Article
Plant Sciences
Yuanyuan Jiang, Jiangrong Peng, Yunpeng Cao, Zhiqiang Han, Ling Zhang, Wenbing Su, Shunquan Lin, Yuan Yuan, Bin Wang, Xianghui Yang, Zhike Zhang
Summary: The study introduced a new method for observing the cell structure of Nicotiana benthamiana, significantly improving imaging by fluorescence microscopy and allowing clear visualization of cellular structures and protein interactions under high-power lens. This method provides insights and references for the study of cell structures and functions in other plants, demonstrating its applicability in live cell visualization through confocal scanning laser microscopy.
PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS
(2021)
Article
Chemistry, Analytical
Ui Jin Lee, Yunkwang Oh, Oh Seok Kwon, Yong-Beom Shin, Moonil Kim
Summary: We developed a highly sensitive and specific bimolecular fluorescence complementation (BiFC)-based influenza A virus (IAV)-sensing system. The system utilizes a galactose/glucose-binding protein (GGBP) with a N-terminal large domain (YN1-172) and a C-terminal small domain (YC173-239) made up of enhanced yellow fluorescence protein (eYFP). The system demonstrated ultra-high sensitivity and selective responses for IAV detection, making it a simple and effective platform for IAV detection without the need for virus culture or RNA extraction processes.
Article
Biochemical Research Methods
Shintaro Ichikawa, Shota Kato, Yuta Fujii, Kazuya Ishikawa, Keiji Numata, Yutaka Kodama
Summary: In this study, we artificially controlled chloroplast-chloroplast interactions in living plant cells using the organelle glue (ORGL) technique. By targeting different fragments of a fluorescent protein to specific locations, we modulated the frequency of chloroplast-chloroplast interactions.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Feng Liu, Huimin Hu, Mengying Deng, Zongqin Xiang, Yuting Guo, Xinmeng Guan, Dong Li, Qinxue Hu, Wenliang Lei, Hongjuan Peng, Jun Chu
Summary: This study reports a bright monomeric near-infrared fluorescent protein, mIFP663, with maximum excitation at 633 nm. It has high cellular brightness and can label critical cellular and viral proteins without affecting subcellular localization and virus replication. Additionally, mIFP663 can be used to improve bimolecular fluorescence complementation and bioluminescent resonance energy transfer systems for detecting protein-protein interactions in living cells.
Article
Plant Sciences
Li Guo, Alina Klaus, Marcel Baer, Gwendolyn K. Kirschner, Silvio Salvi, Frank Hochholdinger
Summary: This study demonstrates the central role of the EGT2 gene and its interaction partners in controlling the root zone-specific transcriptomic reprogramming of barley roots upon gravistimulation. The genes directly interacting with EGT2 are involved in cell wall and reactive oxygen species-related processes. These findings are important for the development of improved crop performance through manipulation of root traits.
Review
Biotechnology & Applied Microbiology
Xueqin Lv, Ke Jin, Guoyun Sun, Rodrigo Ledesma-Amaro, Long Liu
Summary: This review presents the pivotal role of microscopy imaging of living cells in metabolic engineering and its applications in industrial microorganisms. It analyzes the advantages and disadvantages of different microscopy technologies and their roles in synthetic biology. Lastly, it discusses the future perspectives of live-cell imaging and its potential in transforming microbial systems.
TRENDS IN BIOTECHNOLOGY
(2022)
Article
Biochemical Research Methods
Anna Yudenko, Anastasia Smolentseva, Ivan Maslov, Oleg Semenov, Ivan M. Goncharov, Vera V. Nazarenko, Nina L. Maliar, Valentin Borshchevskiy, Valentin Gordeliy, Alina Remeeva, Ivan Gushchin
Summary: Protein-fragment complementation assays are commonly used to study protein-protein interactions. The development of CagFbFP, a small thermostable FbFP based on a LOV domain-containing protein, provides a split fluorescent reporter that can be used to investigate protein-protein interactions in anaerobic conditions without the need for exogenous fluorophores. This advancement in fluorescent protein technology may lead to further development of LOV and PAS domain-based fluorescent reporters and optogenetic tools.
ACS SYNTHETIC BIOLOGY
(2021)
Article
Horticulture
Yingying Wang, Yong 'an Zhang, Yanxiao Dong, Dalu Li, Suli Shi, Shaohang Li, Linzhi Li, Yongjun He, Jianyong Li, Huoying Chen, Haiyan Ge, Yang Liu
Summary: In this study, an efficient protocol for protoplast isolation and transfection in eggplant was established through optimizing enzymatic conditions. This protocol provided high yield and viability of protoplasts, and the PEG-mediated transient transformation system showed over 50% transfection efficiency. The developed method was applied for gene function verification and molecular breeding research.
SCIENTIA HORTICULTURAE
(2022)
Article
Biochemistry & Molecular Biology
Yoshiko Maeda, Tadashi Watanabe, Taisuke Izumi, Kazushi Kuriyama, Shinji Ohno, Masahiro Fujimuro
Summary: This study used the bimolecular fluorescence complementation (BiFC) assay to analyze the interaction between KSHV's ORF18 and ORF30. The results identified four amino acid residues in ORF18 that are crucial for its interaction with ORF30. The AI system AlphaFold2 also supported the importance of these residues. These findings provide important insights into the structural characterization of vPIC protein-protein interactions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Sarai Castro-Bustos, Israel Maruri-Lopez, Maria Azucena Ortega-Amaro, Mario Serrano, Cesare Ovando-Vazquez, Juan Francisco Jimenez-Bremont
Summary: This study analyzed the subcellular localization and potential interactors of the Arabidopsis thaliana glycine-rich domain protein 2 (AtGRDP2). It was found that the AtGRDP2 protein is localized in the cytosol and nucleus, and the DUF1399 or RRM domains are sufficient for nuclear localization. Furthermore, heterodimeric interactions between AtGRDP2 and specific proteins were observed in different compartments, suggesting the involvement of AtGRDP2 in post-transcriptional processes.
CELL STRESS & CHAPERONES
(2022)
Article
Biotechnology & Applied Microbiology
Jingluan Han, Kun Ma, Huali Li, Jing Su, Lian Zhou, Jintao Tang, Shijuan Zhang, Yuke Hou, Letian Chen, Yao-Guang Liu, Qinlong Zhu
Summary: Fluorescent tagging protein localization (FTPL) and bimolecular fluorescence complementation (BiFC) are popular tools for studying protein localization and interactions in plant cells. This study developed an efficient, modular all-in-one fluorescent fusion protein (AioFFP) vector toolbox, which facilitates gene cloning and integration of target gene expression units, promoting functional genomics research in plants.
PLANT BIOTECHNOLOGY JOURNAL
(2022)
Review
Biotechnology & Applied Microbiology
Chih Hung Lo
Summary: Tau protein plays a crucial role in physiological functions but is also implicated in Alzheimer's disease and tauopathies. Recent studies have highlighted soluble tau oligomers as the primary toxic species. The use of cellular biosensor technology has advanced our understanding of tau oligomerization and aggregation, providing insights into tau conformational ensembles and propagation of pathology.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Katsiaryna Shamayeva, Karin Spurna, Natalia Kulik, Deepika Kale, Oksana Munko, Pavel Spurny, Vasilina Zayats, Jost Ludwig
Summary: The study utilized partial gene deletions to create Trk1 fragments with varying numbers of MPM motifs, and found that proteins containing only one or two MPM motifs can interact with each other and assemble with other subunits to form a functional K+ translocation system.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2021)
Article
Genetics & Heredity
Ashley S. Denney, Andrew D. Weems, Michael A. McMurray
Summary: Life necessitates the oligomerization of individual proteins into higher-order assemblies with the help of molecular chaperones. Missense mutations can disrupt protein folding and lead to disease, but some mutations are kinetically delayed to prevent interference with wild-type function. The involvement of chaperones, kinetic folding delays, and oligomerization dynamics were further explored in tumor-derived mutants of the tumor suppressor p53, with findings suggesting temperature sensitivity and Hsp90 regulation.
G3-GENES GENOMES GENETICS
(2021)
Article
Biology
Sherry T. Shu, Wing Fai Li, Thomas E. Smithgall
Summary: This study combines BiFC and immunofluorescence staining to characterize changes in host cell tyrosine kinase activity in the presence of the HIV-1 virulence factor Nef. The findings show that interaction of Nef with Itk and Btk leads to increased kinase activity, which can be evaluated through image analysis.
Article
Cell Biology
Ying Zhang, Pengcheng Wang, Wanchen Shao, Jian-Kang Zhu, Juan Dong
DEVELOPMENTAL CELL
(2015)
Article
Biochemistry & Molecular Biology
Yingnan Hou, Xinyang Guo, Philipp Cyprys, Ying Zhang, Andrea Bleckmann, Le Cai, Qingpei Huang, Yu Luo, Hongya Gu, Thomas Dresselhaus, Juan Dong, Li-Jia Qu
Article
Biochemistry & Molecular Biology
Ying Zhang, Xiaoyu Guo, Juan Dong
Article
Plant Sciences
Ying Zhang, Dominique C. Bergmann, Juan Dong
JOURNAL OF EXPERIMENTAL BOTANY
(2016)
Article
Plant Sciences
Lei Guo, Li Jiang, Ying Zhang, Xiu-Li Lu, Qi Xie, Dolf Weijers, Chun-Ming Liu
Review
Biochemistry & Molecular Biology
Ying Zhang, Chun-Ming Liu, Anne-Mie C. Emons, Tijs Ketelaar
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2010)
Article
Plant Sciences
Ying Zhang, Richard Immink, Chun-Ming Liu, Anne Mie Emons, Tijs Ketelaar