Article
Biochemistry & Molecular Biology
Muriel Rigolet, Nicolas Buisine, Marylou Scharwatt, Evelyne Duvernois-Berthet, Daniel R. Buchholz, Laurent M. Sachs
Summary: Thyroid hormones and glucocorticoids play a role in cell-cycle regulation through the joint regulation of genes in the liver of tadpoles.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Endocrinology & Metabolism
Bidisha Paul, Rejenae Dockery, Valery M. Valverde, Daniel R. Buchholz
Summary: Corticosteroids play a critical role in development and stress responses in vertebrates. A new gene called frzb has been identified as a marker for corticosterone response in frog tadpole tails, making it useful for hormone treatments and endocrine disruption studies.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Ecology
Marissa Fabrezi, Julio Cesar Cruz
Summary: Studies on the effects of thyroid hormones on larval development in frogs provide insights into the genetic and hormonal mechanisms driving growth and transitions throughout development. Mesocosm experiments offer realistic data on environmental conditions, critical for understanding anuran larval development and endocrine disruption. Thyroid axis disruption, specifically with methimazole and thyroxine, influences developmental rates and musculoskeletal morphogenesis in premetamorphic stages of tadpoles.
FRONTIERS IN ECOLOGY AND EVOLUTION
(2021)
Article
Biochemistry & Molecular Biology
Po-Keng Cheng, Hsin-Chang Chen, Pao-Lin Kuo, Jung-Wei Chang, Wan-Ting Chang, Po-Chin Huang
Summary: This study investigated the association between oxidative and nitrosative stress and thyroid hormones, finding that an imbalance of oxidative and nitrosative stress may alter thyroid hormone homeostasis during pregnancy, which could affect embryonic and fetal development.
Article
Neurosciences
Keith T. Sillar, John Simmers, Denis Combes
Summary: The transition from larval to adult locomotion in Xenopus laevis involves a shift from axial to appendicular swimming, with intermediate stages where the tail and hindlimbs contribute to propulsion. The control of hatchling tadpole swimming is mediated by an axial central pattern generator (CPG) that rapidly matures during early larval life. During metamorphosis, a new appendicular CPG, initially driven by the axial system, takes control of the developing limbs, allowing both systems to operate together or independently. Neuromodulation plays a crucial role throughout, with key modulators switching from inhibition to facilitation of locomotion. Temperature also affects locomotor networks and poses a risk to aquatic poikilotherms like amphibians. Anurans, unlike salamanders, have evolved a different locomotor control strategy through the thyroid hormone pathway.
CURRENT OPINION IN NEUROBIOLOGY
(2023)
Article
Food Science & Technology
Yueting Ge, Yuhui Yang, Yuge Jiang, Chuanxing Feng, Bowen Li, Jin Sun, Xue Tang, Yonghui Shi, Guowei Le
Summary: Recent studies have found that high oxidative injury pork and Dityr intake induce oxidative stress and inflammation, impairing thyroid function and peripheral metabolism, leading to a decrease in circulating and liver thyroid hormones and induced hepatic steatosis.
MOLECULAR NUTRITION & FOOD RESEARCH
(2022)
Article
Environmental Sciences
Lauris Evariste, Antoine Mottier, Eric Pinelli, Emmanuel Flahaut, Laury Gauthier, Florence Mouchet
Summary: The interest in graphene-based nanomaterials (GBMs) is increasing globally due to their innovative applications, but concerns about their potential adverse effects on the environment and endocrine disruption potential are also growing. A study found that graphene oxide (GO) and reduced graphene oxide (rGO) have different effects on amphibian endocrine systems, with GBMs potentially increasing the bioavailability and toxicity of other pollutants through hormone adsorption. This highlights the need for further research on the environmental impact and potential risks associated with GBMs.
Article
Environmental Sciences
Yiming Yao, Mengqi Li, Liyang Pan, Yishuang Duan, Xiaoyu Duan, Yongcheng Li, Hongwen Sun
Summary: This study found that maternal exposure to organophosphate esters (OPEs) may affect fetal thyroid function, especially in female infants. The oxidative stress marker 8-OHdG may play a key role in the association between DPHP and neonatal TSH levels, while MDA has a suppressing effect on the associations between mOPEs and neonatal TSH.
ENVIRONMENT INTERNATIONAL
(2021)
Article
Biochemistry & Molecular Biology
Ninoslav Djelic, Suncica Borozan, Vesna Dimitrijevic-Sreckovic, Nevena Pajovic, Milorad Mirilovic, Helga Stopper, Zoran Stanimirovic
Summary: This study investigated the impact of triiodothyronine (T-3) on DNA damage and oxidative stress in peripheral blood mononuclear cells (PBMCs) at different stages of diabetes. The results showed that prediabetic and diabetic patients were more sensitive to T-3, exhibiting elevated DNA damage, inhibition of catalase, and increased levels of TBARS and LDH. Obese patients showed similar responses except for DNA damage.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biology
Yaxin Shi, Yang Shi, Wenyao Ji, Xike Li, Zhiyi Shi, Jilun Hou, Wenjuan Li, Yuanshuai Fu
Summary: In this study, the role of thyroid hormones (TH) in visual remodeling during Japanese flounder (Paralichthys olivaceus) metamorphosis was investigated through cellular molecular biology experiments. The results showed that the expression of the five opsin genes of the flounder were highest in eye tissue and varied with the metamorphosis process. The expression of rh1, sws2a beta and lws was positively regulated by exogenous TH, but inhibited by thiourea (TU) compared to the control group. Meanwhile, T3 levels in flounder larvae were increased by TH and decreased by TU. Based on the differences in the expression of the three isoforms of the thyroid hormone receptor (TR) (Tr alpha a, Tr alpha b and Tr beta), it was further hypothesized that T3 may directly or indirectly regulate the expression of sws2a beta through Tr alpha a. This study demonstrates the regulatory role of TH in opsins during flounder metamorphosis and provides a basis for further investigation on the molecular mechanisms underlying the development of the retinal photoreceptor system in flounders.
Article
Toxicology
David T. Han, Weichen Zhao, Wade H. Powell
Summary: Frog metamorphosis is controlled by thyroid hormone and glucocorticoids, and is susceptible to disruption by 2,3,7,8-tetrachlorodibenzo-p-dioxin. The immediate early gene klf9 serves as a key regulator in the expression changes during metamorphosis, and can be synergistically induced by both the hormones. Binding of AHR to upstream AHREs represents an early key event in TCDD's disruption of klf9 expression and metamorphosis.
TOXICOLOGICAL SCIENCES
(2022)
Article
Marine & Freshwater Biology
Amandine Aviles, Katrine Hulgard, John W. Green, Annette Duus, Bente Holbech, Jane E. Morthorst
Summary: Several chemicals have been found to disrupt the thyroid hormone system in mammals, resulting in neurodevelopmental impairments, as well as causing development and growth disorders in fish and amphibians. However, identifying these chemicals is challenging and current assessment methods only rely on in vivo tests with mammals and amphibians. This study aimed to investigate the effects of two model thyroid hormone disrupting chemicals on a native European amphibian species, and found differences in sensitivity compared to the commonly used African clawed frog Xenopus laevis/tropicalis.
AQUATIC TOXICOLOGY
(2022)
Article
Fisheries
Muhammad Riadul Haque Hossainey, Amulya Yaparla, Zarafsha Uzzaman, Tyler Moore, Leon Grayfer
Summary: Comparisons between tadpole and frog immune cells reveal cytological and transcriptional differences, suggesting distinct roles in host defense and pathogen infection for different developmental stages.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2023)
Article
Environmental Sciences
Xiaoqiang Gao, Xi Wang, Xinyi Wang, Yingying Fang, Shuquan Cao, Bin Huang, Haibin Chen, Rui Xing, Baoliang Liu
Summary: Exposure to ammonia can cause immune and neuroendocrine changes in fish, leading to oxidative damage, plasma immune suppression, and disruption of the endocrine and neurotransmitter systems.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Biochemistry & Molecular Biology
Shouhong Wang, Yuki Shibata, Liezhen Fu, Yuta Tanizaki, Nga Luu, Lingyu Bao, Zhaoyi Peng, Yun-Bo Shi
Summary: Animal regeneration is a natural process that relies on the level of thyroid hormone (T3), which is responsible for the loss of regenerative ability in many organs during development. The metamorphic stages in amphibians, similar to postembryonic development in humans, provide a good model to study the gradual loss of regenerative ability and the role of T3 in this process.
CELL AND BIOSCIENCE
(2023)
Article
Environmental Sciences
Jody L. Heerema, Caren C. Helbing, Greg G. Pyle
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2018)
Article
Behavioral Sciences
Jody L. Heerema, Kevin W. Jackman, Rachel C. Miliano, Linda Li, Tristan S. M. Zaborniak, Nik Veldhoen, Graham van Aggelen, Wayne J. Parker, Gregory G. Pyle, Caren C. Helbing
HORMONES AND BEHAVIOR
(2018)
Article
Engineering, Environmental
Jacob J. Imbery, Craig Buday, Rachel C. Miliano, Dayue Shang, Jessica M. Round, Honoria Kwok, Graham Van Aggelen, Caren C. Helbing
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2019)
Article
Multidisciplinary Sciences
Jared Hobbs, Jessica M. Round, Michael J. Allison, Caren C. Helbing
Article
Multidisciplinary Sciences
Caren C. Helbing, S. Austin Hammond, Shireen H. Jackman, Simon Houston, Rene L. Warren, Caroline E. Cameron, Inanc Birol
SCIENTIFIC REPORTS
(2019)
Article
Environmental Sciences
Lorissa M. Corrie, Meaghan N. Kempe, Oxana Blajkevitch, Dayue Shang, Caren C. Helbing
Summary: The study shows that dioctyl sodium sulfosuccinate (DOSS) can disrupt thyroid hormone (TH) signaling in American bullfrogs, affecting their developmental process. DOSS disrupted the expression of TH-responsive genes in liver and tail fin tissue of tadpoles, with different patterns of effects observed at various concentrations.
ARCHIVES OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
(2021)
Article
Environmental Sciences
Ethan A. Abbott, Caren C. Helbing
Summary: The study found that sucralose altered the expression of fundamental thyroid hormone-response genes in premetamorphic American bullfrog tadpoles, indicating potential impacts of environmental sucralose exposure on amphibian metamorphosis. Further investigations into the cumulative effects of sucralose exposure are warranted.
ARCHIVES OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
(2021)
Article
Environmental Sciences
Anita A. Thambirajah, Michael G. Wade, Jonathan Verreault, Nicolas Buisine, Veronica A. Alves, Valerie S. Langlois, Caren C. Helbing
Summary: This critical review highlights the complexity of Endocrine Disrupting Chemicals interfering with Thyroid Hormone function through interactions with other hormonal axes, and emphasizes the lack of data generated in non-mammalian vertebrate classes. With the availability of more genomics-based resources, better identification and delineation of EDC effects, modes of action, and identification of effective biomarkers suitable for HPT disruption is possible. The effects of EDCs are likely to cascade into a plurality of complex physiological effects, beyond the few variables tested within research studies.
ENVIRONMENTAL RESEARCH
(2022)
Article
Infectious Diseases
Diana Lin, Darcy Sutherland, Sambina Islam Aninta, Nathan Louie, Ka Ming Nip, Chenkai Li, Anat Yanai, Lauren Coombe, Rene L. Warren, Caren C. Helbing, Linda M. N. Hoang, Inanc Birol
Summary: Antibiotic resistance is a global health crisis, and alternative antimicrobial therapeutics are urgently needed. This study presents rAMPage, a bioinformatics discovery platform, to identify AMP sequences from RNA-seq datasets. The researchers used rAMPage on 84 publicly available RNA-seq datasets, identifying 1137 possible AMPs, with 1024 deemed novel. Testing 21 selected peptide sequences, seven of them showed high antimicrobial activity.
Article
Infectious Diseases
Amelia Richter, Darcy Sutherland, Hossein Ebrahimikondori, Alana Babcock, Nathan Louie, Chenkai Li, Lauren Coombe, Diana Lin, Rene L. Warren, Anat Yanai, Monica Kotkoff, Caren C. Helbing, Fraser Hof, Linda M. N. Hoang, Inanc Birol
Summary: Antimicrobial peptides (AMPs) are promising biological molecules for developing new therapeutics against antimicrobial resistance. In this study, 51 AMP candidates were bioinformatically identified and characterized. These AMPs demonstrated activity against bacterial pathogens and low toxicity to human cells. Sequence and structural analysis also predicted their antimicrobial activity.
Article
Marine & Freshwater Biology
Anita A. Thambirajah, Rachel C. Miliano, Ethan A. Abbott, Craig Buday, Dayue Shang, Honoria Kwok, Caren C. Helbing
Summary: Oil spills in high traffic coastal environments have significant impacts on marine ecosystems and the dependent commercial and social interests. It is crucial to develop effective tools to monitor oil contamination and remediation in a timely manner due to the ongoing reliance on marine fuels. This study demonstrates the value of using the cyp1a1 transcript as a sensitive indicator for tracking exposure and recovery from oil contamination.
AQUATIC TOXICOLOGY
(2023)
Article
Fisheries
Morgan D. Hocking, Jeffrey C. MacAdams, Michael J. Allison, Lauren C. Bergman, Robert Sneiderman, Ben F. Koop, Brian M. Starzomski, Mary L. Lesperance, Caren C. Helbing
Summary: A current challenge in environmental DNA (eDNA) applications is accounting for environmental background in surveys. This study conducted two controlled experiments using a coho salmon eDNA assay, demonstrating a novel method to detect target species eDNA in the presence of background and providing a statistically robust assessment for eDNA surveys.
Article
Biology
Michael J. Allison, Jessica M. Round, Lauren C. Bergman, Ali Mirabzadeh, Heather Allen, Aron Weir, Caren C. Helbing
Summary: The study evaluated the quality of filter-immobilized eDNA desiccated with silica gel under different storage conditions for over a year using targeted quantitative real-time polymerase chain reaction (qPCR)-based assays. The results showed that silica gel outperformed ethanol preservation at preventing a decrease in eDNA sample quality at higher temperatures, and that long-term storage of eDNA filters with silica gel beads should be at -20 degrees C to maintain sample integrity.
BMC RESEARCH NOTES
(2021)
Article
Marine & Freshwater Biology
Susie S. Y. Huang, Jonathan P. Benskin, Nik Veldhoen, Bharat Chandramouli, Heather Butler, Caren C. Helbing, John R. Cosgrove
AQUATIC TOXICOLOGY
(2017)
Article
Chemistry, Analytical
Grace Park, Pamela Brunswick, Honoria Kwok, Maxine Haberl, Jeffrey Yan, Ceara MacInnis, Marcus Kim, Caren Helbing, Graham van Aggelen, Dayue Shang
ANALYTICAL METHODS
(2018)
Article
Biochemistry & Molecular Biology
Diana Medina-Felix, Francisco Vargas-Albores, Estefania Garibay-Valdez, Luis Rafael Martinez-Cordova, Marcel Martinez-Porchas
Summary: In this research, the effects of Nocardia infection on fish gastrointestinal microbiota were analyzed. It was found that the infection led to decreased survival rate, severe damage to the stomach microbiota, and a significant increase in Proteobacteria. A negative correlation network between Proteobacteria and other important phyla was observed. Therefore, Nocardia sp. is an emerging pathogen capable of inducing dysbiosis and causing significant mortalities.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS
(2024)
Article
Biochemistry & Molecular Biology
Lele Wu, Wen Sun, Jiale Zhou, Yaolin Li, Jun Li, Zongcheng Song, Changbin Song, Shihong Xu, Xinlu Yue, Xian Li
Summary: The study finds that red light induces dichromatic skin pigmentation in turbot juveniles, with some individuals displaying black coloration and others displaying lighter skin. The upregulated gene expressions related to melanin synthesis and the involvement of the nervous system in spectral environment-driven color regulation are both crucial factors.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS
(2024)