Article
Chemistry, Applied
Keysson V. Fernandes, Elisa D. C. Cavalcanti, Eliane P. Cipolatti, Erika C. G. Aguieiras, Martina C. C. Pinto, Fernanda A. Tavares, Priscila R. da Silva, Roberto Fernandez-Lafuente, Sara Arana-Pena, Jose Carlos Pinto, Charles L. B. Assuncao, Jose Andre C. da Silva, Denise M. G. Freire
Summary: This study successfully synthesized biolubricants from by-products of soybean oil processing using the lipase from Candida rugosa, showing high conversion rates and improved efficiency through immobilization. The products demonstrated good lubricant properties, indicating a potential environmentally friendly alternative to petroleum-based lubricants.
Article
Biochemistry & Molecular Biology
Evan A. Bisirri, Thaiesha A. Wright, Daniel K. Schwartz, Joel L. Kaar
Summary: Protein-polymer conjugation is a useful approach to improve protein stability and performance, but polymer-polymer interactions can also affect the properties of polymer-modified proteins. This study demonstrates that by adjusting the ratio of polymers, the productivity of lipase can be optimized, although this may lead to a trade-off between activity and stability.
Article
Agriculture, Multidisciplinary
Yifei Zhang, Guangzheng Ma, Shushu Wang, Binbin Nian, Yi Hu
Summary: This study achieved efficient immobilization of Candida rugosa lipase on commercial macroporous resins, resulting in an efficient catalyst for the synthesis of phytosterol esters. A solvent-free strategy using oleic acid as the esterification reaction medium yielded a high yield of ester synthesis. This immobilization strategy provides new possibilities for the high-efficiency production of pine sterol esters and other food source nutrients.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2023)
Article
Biotechnology & Applied Microbiology
Shushu Wang, Shan Li, Runtang Liu, Wei Zhang, Huajin Xu, Yi Hu
Summary: In this study, Candida rugosa lipase (CRL) was activated and covalently immobilized on a nanocomposite material using surfactants. The nanocomposite material, fabricated by combining magnetic nanoparticles with chitosan, proved to be an ideal support for enzyme immobilization. The immobilized CRL showed improved stability and reusability.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Nanoscience & Nanotechnology
Yongfa Cheng, Oi-Ming Lai, Chin-Ping Tan, Worawan Panpipat, Ling-Zhi Cheong, Cai Shen
Summary: Immobilization can improve the stability and reusability of lipases, but may also lead to conformational changes that decrease catalytic activity. In this study, grafting proline onto UIO-66 crystals for immobilization of Candida rugosa lipase showed enhanced enzyme activity and better recycling capabilities, indicating improved catalytic efficiency.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Materials Science, Multidisciplinary
Nengwen Gao, Jing Liu, Xiaoli Wang, Yu Zhang
Summary: This study investigates the immobilization of lipase on ceramic membranes using modification and cross-linking methods. The immobilized lipases show higher catalytic activity and stability, making them suitable for applications in high-temperature and alkaline environments.
JOURNAL OF MATERIALS SCIENCE
(2023)
Article
Materials Science, Biomaterials
Hongbo Suo, Huining Geng, Lu Zhang, Guoyun Liu, Hui Yan, Rui Cao, Jiahao Zhu, Yi Hu, Lili Xu
Summary: Enzymatic esterification is an important process in chemistry and biotechnology. In this study, lipase was immobilized on an ionic liquid-modified magnetic metal-organic framework and used for the synthesis of isoamyl acetate. The immobilized lipase showed higher activity, anti-denaturant ability, and reusability compared to other samples. The synthesized composite material exhibited good potential in lipase immobilization with enhanced catalytic ability and stability.
JOURNAL OF MATERIALS CHEMISTRY B
(2023)
Article
Materials Science, Multidisciplinary
Elif Ozyilmaz, Ozge Caglar, Idris Sargin, Gulsin Arslan
Summary: The study successfully prepared a new support material for encapsulating Candida rugosa lipase by combining edible mushroom-derived CQDs and ZIF-8 crystals, with higher enzyme loading efficiency compared to in-situ encapsulation. Structural, morphological, and spectrophotometric analyses showed that CQDs and the enzyme were physically entrapped within the three-dimensional structures of ZIF-8.
MATERIALS TODAY COMMUNICATIONS
(2022)
Article
Chemistry, Physical
Julio Cesar Mendez, Ulises Arellano, Silvia Solis, Jin An Wang, Lifang Chen
Summary: This study investigated the immobilization of Candida Rugosa lipase (CRL) on Ca doped Kit-6 solid through adsorption. The effects of various experimental parameters on the adsorption of CRL were studied. It was found that 0.5Ca/Kit-6 showed the highest CRL immobilization capacity and stability. In the transesterification of coconut oil to biodiesel, the CRL/0.5Ca/Kit-6 catalyst exhibited better catalytic performance under weak basic conditions.
MOLECULAR CATALYSIS
(2022)
Article
Chemistry, Physical
Nurfadhila Nasya Ramlee, Rosli Md Illias, Roshanida A. Rahman, Susilawati Toemen, Rangabhashiyam Selvasembian, Rabi'atul Adawiyah Ahmad, Nor Hasmaliana Abdul Manas, Nur Izyan Wan Azelee
Summary: Enzyme immobilization on inorganic materials is gaining more attention due to the potential characteristics of high surface area-to-volume ratios, increasing enzyme loading efficiency. In this study, Candida rugosa lipase was successfully immobilized on a metal oxide hybrid support, showing high thermal stability and wide pH stability range. The immobilized enzyme also demonstrated high tolerance in solvents compared to free enzyme.
Article
Biochemistry & Molecular Biology
Otavio Domingues, Daniela Remonatto, Leticia Karen dos Santos, Julian Paul Martinez Galan, Danilo Luiz Flumignan, Ariela Veloso de Paula
Summary: This study successfully prepared functionalized maghemite nanoparticles for immobilization of Candida rugosa lipase (CRL) and evaluated its potential in the hydrolysis of waste cooking oil (WCO) and biodiesel production. The immobilized derivative showed high catalytic activity, and after immobilization, the nanoparticles became more dispersed, which is advantageous for catalytic reactions.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2022)
Article
Biology
Prasanna J. Patil, Subodh A. Kamble, Maruti J. Dhanavade, Xin Liang, Chengnan Zhang, Xiuting Li
Summary: This study used molecular modeling approaches to investigate the interaction between CRL and the specific MOF ZIF-8. The results of molecular docking analysis showed that ZIF-8 was located at the active site pocket of CRL and formed hydrogen bonds with specific amino acids. The MD simulation analysis demonstrated the stability of the CRL-ZIF-8 docked complex and the maintenance of all interactions throughout the simulation. These findings contribute to the understanding of the molecular mechanisms and potential applications of immobilized CRL for various industries.
Article
Chemistry, Physical
Xiaoxu Yang, Yan Zhang, Huimin Pang, Sheng Yuan, Xuxia Wang, Zhiming Hu, Qinghua Zhou, Yaojia He, Yunjun Yan, Li Xu
Summary: The study successfully produced biodiesel from tallow seed oil by codisplaying CRL1 and ROL on the cell surfaces of P. pastoris whole-cell catalysts. The activity of codisplaying strains was significantly higher than that of single-displayed enzymes, and using codisplayed enzymes for catalysis can increase the production of methyl esters.
Article
Biochemical Research Methods
Mehdi Mohammadi, Mansour Shahedi, Faezeh Ahrari, Mostafa Mostafavi, Zohreh Habibi, Maryam Yousefi
Summary: The authors propose a simple and cost-efficient approach for the covalent coupling or intermolecular cross-linking of enzymes. This method is versatile and offers operational simplicity, high efficiency, and a notable reduction in time compared to alternative strategies.
Article
Chemistry, Physical
Chaonan Jin, Ning Li, En Lin, Xuepeng Chen, Ting Wang, Yan Wang, Mingfang Yang, Wansheng Liu, Jiangyue Yu, Zhenjie Zhang, Yao Chen
Summary: This study developed a photoenzymatic platform for asymmetric catalysis by immobilizing wheat germ lipase (WGL) onto rationally designed photocatalytic porphyrinic covalent organic frameworks (COFs). The WGL@COFs photobiocatalysts demonstrated high enzymatic activity and good operational stability, and exhibited excellent performance for an enantioselective Mannich reaction under visible light irradiation. This work provides a general and efficient strategy for fabricating photobiocatalysts that enable highly efficient photoenzymatic asymmetric catalysis using COFs as photocatalytic platforms for enzyme immobilization.
Review
Chemistry, Multidisciplinary
Noelia Losada-Garcia, Ivan Rodriguez-Oliva, Milica Simovic, Dejan Bezbradica, Jose M. Palomo
Editorial Material
Chemistry, Physical
Jose M. Palomo
Article
Chemistry, Physical
Noelia Losada-Garcia, Alba Rodriguez-Otero, Jose M. Palomo
Article
Chemistry, Multidisciplinary
Caitlin McCarthy, Noelia Losada-Garcia, Jose M. Palomo
Article
Biochemistry & Molecular Biology
Jana Brabcova, Alicia Andreu, David Aguilera, Zaida Cabrera, Blanca de las Rivas, Rosario Munoz, Jose M. Palomo
Summary: In this study, materials with carboxylic groups were functionalized with geranyl-amine molecules using an EDC/NHS strategy, and applied for site-selective immobilization of proteins. Different strategies were explored, including reversible hydrophobic immobilization and covalent attachment, resulting in significant increases in activity and successful selective immobilization under various conditions.
Editorial Material
Chemistry, Physical
Cesar Mateo, Jose M. Palomo
Review
Chemistry, Multidisciplinary
Noelia Losada-Garcia, Carla Garcia-Sanz, Alicia Andreu, Trinidad Velasco-Torrijos, Jose M. Palomo
Summary: Viruses, highly infectious pathogens, cause a high death toll with a lack of effective drugs, necessitating swift and accurate diagnosis to prevent rapid spread. Nanotechnology-based strategies using glycans offer fast, precise virus identification and inhibition, enhancing patient care with minimal invasiveness and extreme sensitivity capabilities.
Article
Chemistry, Medicinal
David Lopez-Tejedor, Rafael Claveria-Gimeno, Adrian Velazquez-Campoy, Olga Abian, Jose M. Palomo
Summary: Tyrosinases extracted and purified from commercial Agaricus bisporus protein extract and white mushrooms demonstrated high antiviral activity against hepatitis C virus. The newly discovered 50 kDa isoform showed even greater antiviral capacity than the well-known 45 kDa isoform. This antiviral activity was directly related to the enzymatic activity of tyrosinases.
Editorial Material
Chemistry, Multidisciplinary
Jose M. Palomo, M. Carmen Galan, Jose Manuel Garcia-Fernandez
Article
Chemistry, Multidisciplinary
Noelia Losada-Garcia, Jannier Carranza, Jose M. M. Palomo
Summary: Cu2O NPs were created in situ on graphene functionalized with Thermomyces lanuginosus lipase (G@TLL), acting as a template and binder, producing a heterogeneous catalyst. The G@TLL-Cu2O hybrid exhibited high catalytic capacity in the degradation of toxic organic compounds such as trichloroethylene (TCE) and Rhodamine B (RhB). The hybrid showed improved catalytic efficiency compared to the unsupported counterpart and successfully achieved complete degradation of RhB in the presence of H2O2.
Article
Biochemistry & Molecular Biology
Clara Ortega-Nieto, Noelia Losada-Garcia, Benevides C. Pessela, Pilar Domingo-Calap, Jose M. Palomo
Summary: In this study, nanostructured copper materials were designed and synthesized to produce efficient and sustainable copper bionanohybrids with catalytical and antimicrobial properties. Different quantities of a reducing agent were added during the synthesis, resulting in copper bionanohybrids with various metallic species, nanoparticles sizes, and structures. The antimicrobial activity of the bionanohybrids was evaluated against different strains of bacteria, with varying efficiencies observed depending on the bacterial strain and the percentage of reduction in the synthesis. Notably, the antimicrobial activity against Mycobacterium smegmatis was better with bionanohybrids synthesized with a lower reduction step.
ACS BIO & MED CHEM AU
(2023)
Review
Chemistry, Multidisciplinary
Clara Ortega-Nieto, Noelia Losada-Garcia, Doina Prodan, Gabriel Furtos, Jose M. Palomo
Summary: Present difficulties in healthcare and the environment have led to the exploration of novel materials to address current challenges. Nanomaterials, particularly metal nanoparticles and carbon-based nanomaterials, show promise in combating bacterial infections and antibiotic resistance. In this review, recent developments in the design and synthesis of various nanomaterials, their applications in antimicrobial performance, and material protection have been highlighted.
Article
Chemistry, Multidisciplinary
Janne M. Naapuri, Noelia Losada-Garcia, Jan Deska, Jose M. Palomo
Summary: Novel hybrids containing silver or gold nanoparticles were synthesized using enzymes or tailor-made enzyme-polymer conjugates in aqueous media at room temperature. The choice of pH, protein, or bioconjugate strongly affected the formation of metallic nanoparticles. These nanobiohybrids exhibited dual enzymatic and metallic activities and showed high applicability for the design of novel reaction cascades.
Review
Chemistry, Physical
Noelia Losada-Garcia, Zaida Cabrera, Paulina Urrutia, Carla Garcia-Sanz, Alicia Andreu, Jose M. Palomo
Article
Chemistry, Physical
Noelia Losada-Garcia, Alba Rodriguez-Otero, Jose M. Palomo
CATALYSIS SCIENCE & TECHNOLOGY
(2020)
