Article
Plant Sciences
Magda F. El-Adl, Taha M. El-Katony, Reham M. Nada
Summary: The study found that the specific ion effects of Na+ and K+ were different on the growth of Ulva lactuca. Ulva lactuca showed better growth with high concentrations of NaCl, while KCl might have adverse effects on photosynthesis and cell wall. Transcription of PM H+-ATPase was induced specifically by high concentrations of Na+, while the induction of PM-Na+/K+-ATPase-like alone could not improve the growth of KCl-treated Ulva lactuca.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Article
Plant Sciences
Qi Chen, Kai Xu, Yan Xu, Dehua Ji, Changsheng Chen, Chaotian Xie, Wenlei Wang
Summary: This study analyzed the function of Na+/K+-ATPase in the response of Neoporphyra haitanensis to salt stress and found that it played a crucial role in maintaining the K+/Na+ homeostasis in the algae. The inhibitor ouabain caused Na+ accumulation and K+ leakage in the cells, disrupting the K+/Na+ balance and leading to decreased photosynthesis and oxidative damage in the algae.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Marlene U. Fruergaard, Ingrid Dach, Jacob L. Andersen, Mette Ozol, Azadeh Shahsavar, Esben M. Quistgaard, Hanne Poulsen, Natalya U. Fedosova, Poul Nissen
Summary: In this study, the crystal structure and functional properties of the E2-BeFx complex of pig kidney Na+,K+-ATPase are investigated. The complex resembles the E2P intermediate and is able to bind ADP/ATP and Na+. The results suggest a relation between E2-BeFx and the E*P intermediate in the functional cycle of Na+, K+-ATPase.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Santiago E. Faraj, Wanda M. Valsecchi, Mariela Ferreira-Gomes, Mercedes Centeno, Elina Malen Saint Martin, Natalya U. Fedosova, Juan Pablo FC. Rossi, Monica R. Montes, Rolando C. Rossi
Summary: The Na+/K+-ATPase is a plasma membrane glycoprotein that exchanges intracellular Na+ for extracellular K+ by hydrolyzing ATP. The asymmetric distribution of Na+ and K+ is essential for cellular life. Current research indicates that Na+ becomes spontaneously occluded in the E1 dephosphorylated form of the Na+/K+-ATPase, showing positive interactions between binding sites. Adding ATP does not increase Na+ occlusion.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Review
Microbiology
Cassiano Felippe Goncalves-de-Albuquerque, Carolina Medina Coeli da Cunha, Leo Victor Grimaldi de Castro, Caroline de Azevedo Martins, Marcos Roberto Colombo Barnese, Patricia Burth, Mauricio Younes-Ibrahim
Summary: In 1915, Inada and Ido identified Leptospira sp. as the pathogen responsible for Weil's Disease, which was later confirmed to cause leptospirosis. The molecular mechanisms and interactions between bacterial components and the host, as well as the systemic dysfunctions, still need to be fully understood. Leptospirosis affects all animal species and involves a cellular mechanism common to all eukaryotes. Na/K-ATPase is targeted by the leptospiral endotoxin, leading to dysfunctions and organ disorders. Additionally, metabolic disorders and inflammatory responses are triggered during leptospirosis, involving various molecular mechanisms. Na/K-ATPase plays a critical role in the disease and can be a potential therapeutic target or an indicator of disease severity.
Article
Cell Biology
Bruno Sterlini, Alessandra Romei, Chiara Parodi, Davide Aprile, Michele Oneto, Anita Aperia, Pierluigi Valente, Flavia Valtorta, Anna Fassio, Pietro Baldelli, Fabio Benfenati, Anna Corradi
Summary: This study reveals that PRRT2 deficiency affects the function of Na+/K+ ATPase (NKA) in neurons, leading to increased clustering of NKA on the plasma membrane, which can be reversed by re-expression of PRRT2. Additionally, PRRT2 deficiency also reduces the NKA-dependent afterhyperpolarization in neurons following high-frequency firing.
CELL DEATH & DISEASE
(2021)
Article
Cell Biology
Rawad Hodeify, Sawsan Kreydiyyeh, Leen Mohammad Jamal Zaid
Summary: The sodium-potassium pump (NKA) consumes a significant amount of energy in animal cells for creating electrochemical gradients of sodium and potassium ions. The regulation of its activity, including its expression, localization, enzymatic activity, and interactions with other proteins, is crucial. However, the mechanisms controlling the trafficking of NKA between the cell membrane and intracellular compartments are poorly understood. This review summarizes previous studies on the regulation of NKA trafficking and its potential endocytic signals, as well as highlights recent research on NKA trafficking and modulation in various cells.
MOLECULAR AND CELLULAR BIOCHEMISTRY
(2023)
Article
Parasitology
Xiao-Fei Shang, Xiao-Lou Miao, Li-Xia Dai, Yu Wang, Bing Li, Hu Pan, Ji-Yu Zhang
Summary: This study confirmed the strong acaricidal activity and inhibitory effect on Na+-K+-ATPase of the novel strophanthidin derivative QXG-1. By chemically modifying specific positions of the strophanthidin molecule, its toxicity against mites can be controlled. The safety of QXG-1 in vitro and in vivo also suggests its potential as a promising acaricidal agent.
VETERINARY PARASITOLOGY
(2021)
Article
Immunology
Jin Zheng, Ping Lan, Meihe Li, Min-chao Kang, Meng Xun, Xiangyun Ma, Mengyao Yan, Dan Sun, Yu Shen, Xinyi Fu, Xiaoming Ding, Xiaofei Yan, Wu-jun Xue
Summary: Reduced Na+/K+-ATPase (NKA) activity and NKA alpha 1 expression are implicated in the pathogenesis of renal diseases. NKA alpha 1 deficiency exacerbates tubulointerstitial lesions and fibrosis in a mouse model of UUO. Activation of NKA alpha 1 by an antibody against its extracellular DR region (DRm217) prevents UUO-induced tubulointerstitial lesions, preserves kidney function, and reduces renal fibrosis.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Chemistry, Medicinal
Lucy Kate Ladefoged, Birgit Schiott, Natalya U. Fedosova
Summary: The study reveals significant differences in the binding properties of different subfamilies of cardiotonic steroids with the Na+,K+-ATPase. While bufadienolides show beneficial effects with K+, they differ from cardenolides which have an antagonistic relationship with K+. Through simulations, it was found that the binding of bufadienolides is influenced by amino acid coordination patterns and the presence of cations, illustrating the complex interplay of factors in these interactions.
JOURNAL OF CHEMICAL INFORMATION AND MODELING
(2021)
Article
Biochemistry & Molecular Biology
Aline Yammine, Lizette Auezova, Gerard Lizard, Helene Greige-Gerges
Summary: The aim of this study was to investigate whether quercetin, resveratrol, or caffeic acid, at nano-and low micromolar concentrations, can improve the activity of Na+/K+- and Ca2+-ATPases in human erythrocyte membranes with excess cholesterol. These polyphenols belong to different chemical classes and are widely present in plant foods.
Article
Food Science & Technology
Zena Wehbe, Sawsan Kreydiyyeh
Summary: The increase in bovine dairy consumption has been associated with the development of inflammatory diseases. Milk exosomes, which contain cytokines elevated in inflammatory bowel disease, are potential causative agents. This study investigates the relationship between exosomes and Na+/K+ ATPase using Caco-2 cells. The exosomes were found to dose and time-dependently increase ATPase activity, mediated by PGE2 and involving EP3 and EP4 receptors. The activation of ATPase was influenced by PKA and affected by EP3 and EP4 action.
Article
Multidisciplinary Sciences
Victoria C. Young, Hanayo Nakanishi, Dylan J. Meyer, Tomohiro Nishizawa, Atsunori Oshima, Pablo Artigas, Kazuhiro Abe
Summary: This study investigates ion transport mechanisms by introducing mutations to convert an electroneutral proton/potassium pump into a prototypical electrogenic sodium/potassium pump, explaining their selectivity and phosphorylation mechanisms.
NATURE COMMUNICATIONS
(2022)
Article
Biology
R. Meldrum Robertson, Christopher D. Moyes
Summary: The study shows that rapid cold hardening provides protection for locusts by activating Na+/K+-ATPase, enhancing in situ NKA activity in the nervous system, as well as affecting other neuronal properties that promote visual processing.
JOURNAL OF EXPERIMENTAL BIOLOGY
(2022)
Article
Ecology
Deena A. Hansen, Amanda S. Williard, Frederick S. Scharf
Summary: By studying the thermal sensitivity of red drum gill Na+/K++ ATPase, we gained insight into the potential temperature effects on osmoregulatory capabilities and overwinter survival of red drum.
JOURNAL OF EXPERIMENTAL MARINE BIOLOGY AND ECOLOGY
(2022)
Article
Toxicology
Giovanni Castaldo, Vyshal Delahaut, Bart Slootmaekers, Lieven Bervoets, Raewyn M. Town, Ronny Blust, Gudrun De Boeck
Summary: This study compared the effects of waterborne copper, zinc, and cadmium on common carp, revealing different toxicities and impacts on ion levels and oxidative stress in the fish organism.
JOURNAL OF APPLIED TOXICOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Prabesh Singh Kunwar, Kusum Parajuli, Samikshya Badu, Bhawani Sapkota, Amit Kumar Sinha, Gudrun De Boeck, Kumar Sapkota
Summary: The study found that the golden mahseer has higher toxicity to chlorpyrifos than dichlorvos, with their combined effect showing antagonism. Exposure to both pesticides led to reduced oxygen consumption and increased opercular movements, with chlorpyrifos treatments showing more persistent effects. Decreased feeding attempts were observed in chlorpyrifos and mixture treatments. The acute toxicity data can be used to assess the maximum tolerance level of golden mahseer to these pesticides, and the sub-lethal end point responses can be applied in monitoring the environmental risk posed by the pesticides to aquatic life.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2021)
Article
Fisheries
Suhaini Mohamad, Hon Jung Liew, Rabiatul Adawiyyah Zainuddin, Sharifah Rahmah, Khor Waiho, Mazlan Abd Ghaffar, Hua Thai Nhan, Jiun-Yan Loh, Leong-Seng Lim, Yumei Chang, Liqun Liang, Gudrun De Boeck
Summary: High temperature and low pH were found to cause severe changes in the gill structure of Hoven's carp, including modifications such as aneurysm, oedema, and hypertrophy. The fish also exhibited coping mechanisms such as epithelial lifting, hyperplasia, and lamellae fusion in response to the changing environment.
JOURNAL OF FISH BIOLOGY
(2021)
Article
Fisheries
Hayden Fischer, Nicholas Romano, Jacob Jones, John Howe, Nilima Renukdas, Amit Kumar Sinha
Summary: The study found that there were no significant differences in the survival, growth, feeding efficiencies, or condition of largemouth bass juveniles cultured in aquaponic systems or recirculating aquaculture systems, despite higher nitrate-N levels in RAS. The growth and root development of spring onion and lemongrass in the aquaponic system were significantly influenced by the presence of media.
Article
Biochemistry & Molecular Biology
M. Pillet, G. Castaldo, E. M. Rodgers, V. Poleksic, B. Raskovic, L. Bervoets, R. Blust, G. De Boeck
Summary: The study found that exposure to an environmentally relevant metal mixture and different temperatures had an impact on the physiological performance of juvenile common carp, with higher temperatures leading to increased cadmium accumulation and the need for higher hematocrit levels to maintain aerobic performance.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2021)
Article
Physiology
Giovanni Castaldo, Marion Pillet, Leen Ameryckx, Lieven Bervoets, Raewyn M. Town, Ronny Blust, Gudrun De Boeck
Summary: The study investigated the effects of exposing common carp to a ternary metal mixture of Cu, Zn, and Cd at different temperatures. The results showed differences in metal accumulation in the tissues, as well as changes in gene expression related to ionoregulation and defensive responses. Additionally, analysis of electrolyte levels did not show significant losses, suggesting compensation through ion uptake from food.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Marine & Freshwater Biology
G. Castaldo, T. Nguyen, R. M. Town, L. Bervoets, R. Blust, G. De Boeck
Summary: In the mixture scenario, Zn inhibited the accumulation of Cd in the fish liver, but this inhibition was temporary in the gills. The delayed accumulation of Zn indicated efficient homeostasis of this essential metal. Compared to a single Zn exposure, the metal mixture resulted in reduced Zn accumulation.
AQUATIC TOXICOLOGY
(2021)
Article
Environmental Sciences
Lies Teunen, Claude Belpaire, Gudrun De Boeck, Ronny Blust, Lieven Bervoets
Summary: The study measured mercury accumulation concentrations in muscle and liver tissue of perch and European eel in Flemish waterbodies in Belgium, revealing influences of different environmental conditions on mercury accumulation in these species. The effects of size and weight on the accumulation and storage of pollutants, as well as differences in mercury concentrations between perch and eel, were also evaluated.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Evolutionary Biology
Federico C. F. Calboli, Vyshal Delahaut, Io Deflem, Pascal Hablutzel, Bart Hellemans, Anna Kordas, Joost A. M. Raeymaekers, Lieven Bervoets, Gudrun De Boeck, Filip A. M. Volckaert
Summary: Using the three-spined stickleback as a model, the study found that mercury pollution in Flanders causes a significant association with at least one locus on Chromosome 4 through sequencing and genome-wide association analysis.
EVOLUTIONARY APPLICATIONS
(2021)
Article
Fisheries
Ivan Loaiza, Gudrun De Boeck, Juan Alcazar, Diego Campos, Susana Cardenas-Alayza, Maria Ganoza, Muriel Gomez-Sanchez, Maria Miglio, Marleen De Troch
Summary: The study reveals a southward increased gradient of delta N-15 along the Peruvian coast and trophic magnification effects of As and Cd at certain locations. In the Peruvian marine food webs, both metal biomagnification and non-biomagnification effects were found, with A. purpuratus consistently fitting the trophic metal magnification or bio-dilution regression models as an intermediate consumer or prey.
JOURNAL OF THE WORLD AQUACULTURE SOCIETY
(2022)
Article
Biochemistry & Molecular Biology
Prabesh Singh Kunwar, Amit Kumar Sinha, Gudrun De Boeck, Kumar Sapkota
Summary: The study found that mixture pesticide treatment resulted in damage to the kidney and liver of juvenile golden mahseer. In this short exposure experiment, the kidney was more sensitive than the liver to pesticide toxicity. Most biomarkers were predominantly affected in the later exposure phase and gradually recovered during the depuration period.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2022)
Article
Fisheries
Shivendra Kumar, Zsuzsanna J. Sandor, Janka Biro, Gergo Gyalog, Amit Kumar Sinha, Gudrun De Boeck
Summary: This study aimed to improve the utilization of plant based feed in common carp by feeding them with plant based diet at an early life stage. The results showed that the nutritional history had a significant impact on nutrient utilization during later life stages, and nutritional programming at an early stage may be an effective strategy for completely replacing fish based diet with plant based diet in common carp.
Article
Ecology
Prabesh Singh Kunwar, Bhawani Sapkota, Samikshya Badu, Kusum Parajuli, Amit Kumar Sinha, Gudrun De Boeck, Kumar Sapkota
Summary: The toxicity of organophosphate pesticides to mrigal fish species was evaluated and it was found that the joint toxicity of these pesticides was less than additive. Behavioral changes were observed in individual and mixture pesticide treatments, which can be used as a non-invasive biomonitoring tool for assessing water quality impact on fish growth and development.
Article
Environmental Sciences
Bealemlay Abebe Melake, Bossissi Nkuba, Thimo Groffen, Gudrun De Boeck, Lieven Bervoets
Summary: This study investigates the distribution of metals and metalloids in water, sediment, and fish tissues of Lake Hawassa in Ethiopia and assesses the resulting health and ecological risks. The results show that the metal concentrations in water do not have a potential adverse effect on aquatic life, but certain metals exceed the probable effect concentration in sediment, suggesting potential toxicity to aquatic organisms. Fish store more metals in their liver than in their muscle, and different fish species have varying levels of metal accumulation. Positive correlations among metals in water and sediment indicate a potential common pollution source.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Biochemistry & Molecular Biology
Sang Hyuck Lee, Mi Ae Kim, Young Chang Sohn
Summary: This study characterized the CCAP signaling system in Pacific abalone and revealed its relationship with the calcium/PKC and cAMP/PKA signal transduction pathways. It provides new insights into the evolutionary origins of the NPS and OT/VP signaling systems in protostomes.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Hanna Scheuffele, Erica V. Todd, John A. Donald, Timothy D. Clark
Summary: Global warming is causing an increase in extreme weather events, affecting ecosystems and the behavior of ectotherms. More research is needed to understand the impact of diurnal thermal variability and improve climate change predictions.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Jaakko Haverinen, Ahmed Badr, Markus Eskelinen, Matti Vornanen
Summary: The acclimatization of certain ectothermic vertebrates to winter conditions involves reduced energy consumption, achieved through decreased movement activity, depression of cellular functions, or switching to anaerobic energy production. This study on crucian carp showed that winter-acclimatized fish exhibited lower metabolic rates and a shift to anaerobic energy production during anoxia. Winter dormancy in crucian carp is achieved through active reduction of energy consumption, a slowed metabolic rate, and a direct response to oxygen absence.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Maria Cruz Sueiro, Cynthia A. Awruch, Gustavo M. Somoza, Walter S. Svagelj, Maria G. Palacios
Summary: According to life-history theory, there might be different immunological strategies between reproductive and non-reproductive periods due to limited resources. Our study on two sympatric marine fish species, rockfish and sandperch, found that reproductive individuals showed lower immune function, but higher levels of natural antibodies in rockfish females, compared to their non-reproductive counterparts. On the other hand, reproductively active sandperch males showed lower levels of natural antibodies and higher neutrophil to lymphocyte ratio and spleen index compared to non-reproductive males. The study highlights the species-specific patterns of immunity and the potential influence of resource limitation and abiotic factors on immune trade-offs in fish.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
James B. Cummins, Dane A. Crossley II
Summary: This study focuses on cardiovascular development in altricial bird species, which has been less studied compared to precocial species. The researchers investigated the cholinergic and adrenergic receptor mediated control of arterial blood pressure and heart rate in the neotropic cormorant. The findings suggest that both cholinergic and adrenergic tone play a role in cardiovascular regulation in embryonic birds.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Ru Xia, Hong-Kang Liu, Xi-Feng Liu, Xin Deng, Chuan-Jie Qin, Yuan-Fa He, Shi-Mei Lin, Yong-Jun Chen
Summary: This study cloned the genes encoding glucokinase and glucose-6-phosphatase catalytic subunit in largemouth bass, revealing their distribution and transcriptional regulation. The results showed that the functions of G6pc and Gck in LMB were highly conserved in evolution, and the interconversion of glucose and G6P in the liver was well regulated at the transcript level under high starch diet, but a futile cycle was induced after a glucose load.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2024)