Review
Biotechnology & Applied Microbiology
Xiaojing Li, Xin Liang, Yanhui Wang, Dashan Wang, Minhua Teng, Hao Xu, Baodong Zhao, Lei Han
Summary: This article reviews the applications of nanomaterials, particularly graphene and its derivatives, in the dental field. It discusses the synthesis methods, structures, and properties of graphene-based materials, and provides an overview of their current dental applications. The challenges and future prospects of graphene-based nanomaterials in dental applications are also discussed.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Dentistry, Oral Surgery & Medicine
Thibault Canceill, Paul Monsarrat, Edouard Faure-Clement, Marie Tohme, Jean-Noel Vergnes, Brigitte Grosgogeat
Summary: The dental practice-based research networks (D-PBRN) play an important role in structuring clinical research in private practices and increasing research scope. The majority of scientific production is from the USA, and D-PBRN helps in studying a higher number of diverse centers, bridging the gap between private practice and research.
JOURNAL OF DENTISTRY
(2021)
Review
Cell Biology
Elisabetta Xue, Antonina Minniti, Tobias Alexander, Nicoletta Del Papa, Raffaella Greco
Summary: Systemic sclerosis is a disease characterized by autoimmune responses, vasculopathy, and tissue fibrosis. Currently available drugs have limited effects, so cellular therapies have become an important area of research.
Review
Medicine, General & Internal
Mehmet Murat Seval, Kazibe Koyuncu
Summary: Stem cells have the potential to be used in regenerative medicine for treating lower urinary tract dysfunction and stress urinary incontinence. However, further research and validation are needed for clinical applications of stem cell therapy.
FRONTIERS IN MEDICINE
(2022)
Article
Cell & Tissue Engineering
John F. Ouyang, Sonia Chothani, Owen J. L. Rackham
Summary: Deep learning plays an important role in the future of stem cell research and requires suitable data generation, close collaboration between experimental and computational researchers, and overcoming challenges of fairness and effectiveness. Only by achieving these conditions can deep learning models be both biologically meaningful and computationally tractable.
Review
Biochemistry & Molecular Biology
Igor Smojver, Ivan Katalinic, Roko Bjelica, Dragana Gabric, Vid Matisic, Vilim Molnar, Dragan Primorac
Summary: The application of mesenchymal stem cells (MSC) in regenerative dentistry is gaining importance. Dental tissue-derived MSCs have characteristics such as multidifferentiation potential, self-renewal capacity, and easy accessibility, making them key players in stem cell-based therapy. Different types of dental stem cells have been discovered and their potential usage is found in various branches of dentistry as well as other medical fields. This review focuses on dental MSC sources and their treatment modalities, with a specific emphasis on temporomandibular joint osteoarthritis (TMJ OA).
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Nela Pilbauerova, Jan Schmidt, Tomas Soukup, Tomas Prat, Kristina Nesporova, Vladimir Velebny, Jakub Suchanek
Summary: This study investigated the use of high-molecular-hyaluronic acid (HMW-HA) as a cryoprotectant for human mesenchymal stem cells (hMSCs) to reduce DMSO concentration in the cryopreservation medium. The results showed that HMW-HA combined with lower DMSO concentrations improved total cell count, viability, and proliferation potential of hMSCs post-cryopreservation, without affecting their differentiation ability.
Review
Gastroenterology & Hepatology
Ensieh Zahmatkesh, Niloofar Khoshdel Rad, Nikoo Hossein-Khannazer, Mehdi Mohamadnejad, Roberto Gramignoli, Mustapha Najimi, Reza Malekzadeh, Moustapha Hassan, Massoud Vosough
Summary: According to the recent updates from the World Health Organization, liver diseases rank as the 12th most common cause of mortality. Orthotopic liver transplantation (OLT) is currently the most effective treatment for end-stage liver diseases. However, due to limitations such as limited organ donors, lifelong immunosuppression, and procedure complexity, cell and cell-derivative therapies have emerged as potential alternatives for liver diseases.
EXPERT REVIEW OF GASTROENTEROLOGY & HEPATOLOGY
(2023)
Article
Dentistry, Oral Surgery & Medicine
Joana Cunha-Cruz, Gregg H. Gilbert, Veerasathpurush Allareddy, David L. Cochran, Jeffrey Fellows, Dorota T. Kopycka-Kedzierawski, MaryAnn Mcburnie, Cyril Meyerowitz, Rahma Mungia, D. Brad Rindal, Valeria V. Gordan
Summary: This study describes the characteristics of dentists in the US National Dental PBRN and their frequency of providing specific dental procedures. The results show that participating dentists have similarities with dentists in general and the network represents a wide range of dentists, patient populations, and treatments offered.
JOURNAL OF DENTISTRY
(2023)
Review
Polymer Science
Andrea Kowalska, Jerzy Sokolowski, Kinga Bociong
Summary: This paper discusses the current knowledge of commercial and alternative photoinitiator systems in dentistry, focusing on mechanisms of polymerization, measurement methods, and factors affecting hardness of dental resins. Despite no adequate replacement for camphorquinone, alternative photosensitizers used in combination with it can improve aesthetic properties and degree of conversion of dental resin.
Review
Cell & Tissue Engineering
Hua-Nien Lee, Cheng Liang, Li Liao, Wei-Dong Tian
Summary: The trend of using biological therapy to regenerate dental pulp and the multi-differentiation potential of dental pulp stem cells have sparked interest in clinical research. Stem cell-based pulp regeneration shows promising potential in clinical therapy.
TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2021)
Review
Cell Biology
Vanessa Barbaro, Filippo Bonelli, Stefano Ferrari, Giulia La Vella, Enzo Di Iorio
Summary: EEC syndrome is caused by mutations in the p63 gene, leading to limbal stem cell failure and visual loss. There are currently no definitive cures, but therapeutic strategies such as gene therapy, cell therapy, and drug therapy are being explored. Further studies are needed to confirm their effectiveness and safety.
Review
Biochemistry & Molecular Biology
Behrouz Mollashahi, Hamid Latifi-Navid, Iman Owliaee, Sara Shamdani, Georges Uzan, Saleh Jamehdor, Sina Naserian
Summary: CRISPR is the most widely used genome editing toolkit that allows for the replacement and modification of DNA and RNA nucleotides. It has been used by researchers to investigate the function of stem cell genes and various gene editing techniques such as knock-out, knock-in, gene activation, and inhibition. This review article discusses recent developments and applications of CRISPR in stem cell research and clinical purposes, as well as different delivery methods and off-target detecting strategies.
Review
Biochemistry & Molecular Biology
Alexandra Roi, Ciprian Roi, Meda Lavinia Negrutiu, Laura Cristina Rusu, Mircea Rivis
Summary: Dental stem cells, such as human periapical cyst mesenchymal stem cells (hPCy-MSCs), derived from the oral cavity, possess multilineage differentiation potential, immunomodulatory activity, and repair capacity. These cells exhibit similar characteristics to mesenchymal stem cells (MSCs) and hold great significance for the development of clinical applications in dentistry.
Review
Materials Science, Multidisciplinary
Asanah Radhi, Dasmawati Mohamad, Fatimah Suhaily Abdul Rahman, Abdul Manaf Abdullah, Habsah Hasan
Summary: Graphene-based nanomaterials (GBNs) have excellent antimicrobial properties by disrupting bacterial cell membrane integrity and generating reactive oxygen species (ROS). The antimicrobial effects of GBNs are influenced by factors such as microbial cell morphology, GBNs characteristics, and concentration. Potential clinical applications of GBNs in dentistry include denture fabrication, restorative treatment, orthodontic treatment, and dental implant treatment.
JOURNAL OF MATERIALS RESEARCH AND TECHNOLOGY-JMR&T
(2021)
Article
Biochemistry & Molecular Biology
Pierfrancesco Pagella, Cesar Nombela-Arrieta, Thimios A. Mitsiadis
Summary: Specific stem cell populations within dental mesenchymal tissues ensure tooth homeostasis and regeneration, with the decision between renewal and differentiation influenced by interactions with stromal cells and extracellular matrix molecules. The Cxcl12 chemokine, a general marker of stromal cells, plays crucial roles in stem cell maintenance, mobilization, and migration. Using Cxcl12-GFP transgenic mice, this study revealed widespread expression of Cxcl12 in intact and injured teeth, highlighting its significance in various dental mesenchymal stem cell niches.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Pierfrancesco Pagella, Laura de Vargas Roditi, Bernd Stadlinger, Andreas E. Moor, Thimios A. Mitsiadis
Summary: This study mapped the transcriptional landscape of various cell populations in human teeth at single-cell resolution, analyzing their stem cell populations and microenvironment in detail. The research revealed significant cellular heterogeneity in dental pulp and periodontium, with similar molecular signatures of stem cell populations but divergent microenvironments. The findings suggest that microenvironmental specificity could be a key factor in functional differences between highly similar stem cells, potentially influencing cell-based dental therapeutic approaches.
Article
Cell & Tissue Engineering
Pierfrancesco Pagella, Laura de Vargas Roditi, Bernd Stadlinger, Andreas E. Moor, Thimios A. Mitsiadis
Summary: The Notch signaling pathway plays a crucial role in mediating interactions between perivascular stem cells and their niches in human dental mesenchymal tissues, influencing the response to traumatic injuries in vivo. These stem cells can be modulated by cues from the microenvironment, highlighting the pivotal importance of Notch signaling in tissue homeostasis and regeneration.
STEM CELLS TRANSLATIONAL MEDICINE
(2021)
Review
Biochemistry & Molecular Biology
Guya Diletta Marconi, Cristina Porcheri, Oriana Trubiani, Thimios A. Mitsiadis
Summary: Three-dimensional culture systems have revolutionized the study of tissue and organ biology, disease modeling, and drug screening. The Notch signaling pathway plays a crucial role in cell fate determination, tissue definition, and maintenance, making 3D cultures a valuable platform to study its activation and modulation. Advances in various 3D culture systems, such as spheroids, organoids, and organ-on-a-chip models, have greatly contributed to understanding the role of Notch signaling in tissue homeostasis, pathology, and regeneration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Oncology
Cristina Porcheri, Thimios A. Mitsiadis
Summary: Head and neck squamous cell carcinoma (HNSCC) is a common type of cancer with a high fatality rate. HNSCC is primarily linked to factors like tobacco use, areca nut/betel quid consumption, alcohol intake, and HPV infection. The high intratumor heterogeneity in HNSCC and other head and neck cancers makes treatment challenging and contributes to drug resistance, emphasizing the need for new therapeutic approaches and drug testing models.
Article
Cell Biology
Chai Foong Lai, Juliet Shen, Anamaria Balic, Pierfrancesco Pagella, Martin E. Schwab, Thimios A. Mitsiadis
Summary: The study identified the widespread distribution of Nogo-A in human teeth, and clarified its significant regulatory role in the development of dental pulp stem cells, indicating its potential application in clinics.
Article
Multidisciplinary Sciences
Thimios A. Mitsiadis, Lucia -Rojo, Anamaria Balic, Silvio Weber, Paul Saftig, Pierfrancesco Pagella
Summary: The Adam10/Notch axis controls dental epithelial cell boundaries, cell fate switch, and proper enamel formation in continuously growing incisors.
Article
Biochemistry & Molecular Biology
Thimios A. Mitsiadis, Pierfrancesco Pagella, Terence D. Capellini, Moya Meredith Smith
Summary: The Notch pathway is involved in cell fate specification and embryonic development. The Jagged2 gene, which encodes a ligand for Notch receptors, is expressed early in tooth development. Mutations in Jagged2 result in abnormal tooth morphology and impaired enamel deposition. The Notch signaling cascade is disrupted in Jagged2 mutant teeth, leading to dental structures more reminiscent of fish enamel. We propose that the increased number of Notch homologues in metazoa enabled the formation and maintenance of distinctive cell fates within organs and tissues during evolution.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Cell Biology
Thimios A. Mitsiadis, Pierfrancesco Pagella, Helder Gomes Rodrigues, Alexander Tsouknidas, Liza L. Ramenzoni, Freddy Radtke, Albert Mehl, Laurent Viriot
Summary: Evolutionary changes in vertebrates are often accompanied by genetic alterations that affect tooth crown shape. The Notch pathway, a highly conserved signaling pathway, plays a crucial role in morphogenetic processes, including tooth development. Experimental studies on developing mouse molars showed that the loss of the Notch-ligand Jagged1 leads to minor modifications in tooth crown shape, similar to those observed during the evolution of Muridae species. RNA sequencing analysis revealed that these alterations are caused by changes in the expression of over 2000 genes, and that Notch signaling interacts with other important morphogenetic networks, such as Wnts and Fibroblast Growth Factors. The findings from this study provide insights into the role of Notch/Jagged1-mediated signaling in dental variations during evolution.
Article
Dentistry, Oral Surgery & Medicine
Anamaria Balic, Dilara Perver, Pierfrancesco Pagella, Hubert Rehrauer, Bernd Stadlinger, Andreas E. Moor, Viola Vogel, Thimios A. Mitsiadis
Summary: In this study, a comprehensive single-cell atlas of carious human teeth was generated using single-cell RNA sequencing analysis and immunostaining. The study found modifications in various cell clusters within the carious dental pulp, including immune cells, mesenchymal stem cells, and fibroblasts. These modifications led to an increase in extracellular matrix components and myofibroblast enrichment. The study also revealed molecular, cellular, and biomechanical alterations in the carious dental pulp, indicative of extensive extracellular matrix remodeling.
INTERNATIONAL JOURNAL OF ORAL SCIENCE
(2023)
Article
Biochemical Research Methods
Pierfrancesco Pagella, Bernd Stadlinger, Thimios A. Mitsiadis
Summary: Teeth and surrounding periodontal tissues are often affected by pathologies, compromising their integrity and life quality. The study of dental pulp and periodontium is challenging due to their associations with highly mineralized tissues, but can be facilitated by single-cell RNA sequencing analysis.
Article
Cell & Tissue Engineering
P. Pagella, A. Cordiale, G. D. Marconi, O. Trubiani, M. Rasponi, T. A. Mitsiadis
Summary: Various factors contribute to dental pathologies, and current clinical approaches using inert materials for tooth restoration do not fully restore physiological function. Although there have been attempts to use dental mesenchymal stem cells for tooth tissue regeneration, the lack of suitable platforms that mimic the complexity of human dental tissues has hindered clinical applications. Efforts have been made to develop innovative systems for in vitro modeling of human tooth physiology.
EUROPEAN CELLS & MATERIALS
(2021)
