Article
Cell Biology
Francesco Paduano, Elisabetta Aiello, Paul Roy Cooper, Benedetta Marrelli, Irina Makeeva, Mohammad Islam, Gianrico Spagnuolo, Davide Maged, Danila De Vito, Marco Tatullo
Summary: Dental stem cells have the potential to differentiate into various cell types and can revert back to a stem-like state after osteogenic differentiation, showing a greater potential for further differentiation. The ability to control dedifferentiation of DSCs under physiological conditions could enhance their therapeutic potential in bone tissue engineering.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Tianyu Chen, Dexuan Xiao, Yanjing Li, Sirong Shi, Xiao Yang, Shuanglin Peng, Bin Guo, Xiaoxiao Cai
Summary: Osteoporosis has a severe impact on the health of elderly individuals, but autologous stem-cell transplantation can help improve bone density and fracture healing abilities. However, osteoporosis can negatively affect the osteogenesis and proliferation abilities of autologous adipose-derived stem cells. A study found that tetrahedral framework nucleic acid can effectively promote the recovery of osteogenic and proliferative potential in osteoporotic ASCs and enhance their bone formation and proliferation.
CHINESE CHEMICAL LETTERS
(2022)
Article
Biotechnology & Applied Microbiology
Hyun-Ho Yun, Seong-Gon Kim, Se-Il Park, Woori Jo, Kyung-Ku Kang, Eun-Joo Lee, Dong-Kyu Kim, Hoe-Su Jung, Ji-Yoon Son, Jae-Min Park, Hyun-Sook Park, Sunray Lee, Hong-In Shin, Il-Hwa Hong, Kyu-Shik Jeong
Summary: Bone regeneration is a complex process influenced by factors like inflammation, tissue interactions, and progenitor cells. Bone grafting is currently the most effective treatment, but it has drawbacks. Adipose-derived stem cells show promise in accelerating bone regeneration.
BIOENGINEERING-BASEL
(2023)
Article
Cell & Tissue Engineering
Wei Ge, Yuan-Chao Sun, Tian Qiao, Hai-Xia Liu, Tao-Ran He, Jun-Jie Wang, Chun-Lei Chen, Shun-Feng Cheng, Paul W. Dyce, Massimo De Felici, Wei Shen
Summary: Based on single-cell RNA sequencing analysis, this study identified multipotent papillary dermal fibroblast progenitors as the main component of skin-derived stem cells (SDSCs). It was found that epidermal growth factor (EGF) signaling plays a crucial role in the proliferation and colony formation of these progenitors. Furthermore, the study optimized a protocol to efficiently induce SDSCs to differentiate into male germline cells, which showed meiotic potential when cultured with testicular organoids.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Cell & Tissue Engineering
Bo-Yoon Park, Dirong Wu, Kyoo-Ri Kwon, Mi-Jin Kim, Tae-Gon Kim, Jun-Ho Lee, Do Young Park, Il-Kug Kim
Summary: This study investigated the inhibitory role of adipose-derived stem cells (ASCs) on capsule formation in a mouse model of peri-implant fibrosis. It was found that injected ASCs can distribute within the peri-implant capsule, reduce capsule thickness, decrease the number of myofibroblasts and macrophages, and lower the mRNA level of fibrogenic genes. Additionally, ASCs can trans-differentiate into vascular endothelial cells, promoting angiogenesis and relieving tissue hypoxia. These findings highlight the potential clinical application of ASCs in preventing capsular contracture after implant-based reconstruction surgery.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Biochemistry & Molecular Biology
Saideh Hemati, Ashrafalsadat Hatamian-Zarmi, Raheleh Halabian, Mohsen Ghiasi, Ali Salimi
Summary: The research indicates that SPG can effectively promote osteogenic differentiation of hADMSCs, enhance osteogenic markers and mineralization, and upregulate the expression of bone-specific genes.
MOLECULAR BIOLOGY REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Aleksandra Kostina, Arseniy Lobov, Daria Semenova, Artem Kiselev, Polina Klausen, Anna Malashicheva
Summary: Despite significant progress in bone tissue regeneration, the early mechanisms of osteogenic differentiation remain poorly understood. Various cells can undergo osteogenic transformation, with Notch signaling playing a key role in this process. Research demonstrates differences in the ability of different cell types to undergo Notch-dependent osteogenic differentiation.
Article
Biotechnology & Applied Microbiology
Min Wang, Yi Guo, Zexing Deng, Peng Xu
Summary: In this paper, a novel organic-inorganic composite hydrogel was designed, which exhibited excellent mechanical properties and promoted stem cell osteogenic differentiation, showing potential application in bone tissue engineering.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Cell Biology
Jingsi Yang, Nan Ding, Dandan Zhao, Yunsheng Yu, Chunlai Shao, Xuan Ni, Zhen-Ao Zhao, Zhen Li, Jianquan Chen, Zheng Ying, Miao Yu, Wei Lei, Shijun Hu
Summary: The study developed a new strategy of using EBSS treatment to mimic in vivo starvation event, promoting the maturation of hESC-CMs. Intermittent starvation through EBSS significantly enhanced structural, metabolic, and electrophysiological maturity of hESC-CMs.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemical Research Methods
Jiekai Yin, Tianyu F. Qi, Yen-Yu Yang, Madeline Vera-Colon, Nicole I. zur Nieden, Yinsheng Wang
Summary: RNA modifications and their associated proteins play a regulatory role in osteogenesis, and this study provides comprehensive quantitative assessment of these proteins during osteogenic differentiation. The down-regulation of certain RNA modification proteins, mainly involved in methylation and pseudouridylation, was found. Protein-protein interaction analysis revealed their significant associations with osteogenesis-related proteins, and gene set enrichment analysis suggested a potential role of METTL1 in osteogenesis through the cytokine network.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Cell & Tissue Engineering
Daniella Da Silva, Anine Crous, Heidi Abrahamse
Summary: Osteoporosis is a common degenerative bone condition, and stem cell regenerative therapy and photobiomodulation have shown promise in treating it. This study found that a combination of photobiomodulation and differentiation inducers stimulated the early osteogenic differentiation of stem cells. However, further research is needed to establish the effectiveness of this novel wavelength combination.
REGENERATIVE THERAPY
(2023)
Article
Chemistry, Physical
Oleg Prymak, Lida E. Vagiaki, Ales Buyakov, Sergei Kulkov, Matthias Epple, Maria Chatzinikolaidou
Summary: The study synthesized porous zirconia and magnesia ceramics with varying compositions through sintering, and investigated the incorporation of Mg atoms into the zirconia lattice. Results showed that increasing magnesia content led to higher Young modulus and osteogenic activity, with ceramics exhibiting higher calcium mineralization values.
Article
Cell Biology
Seung-Cheol Choi, Ha-Rim Seo, Long-Hui Cui, Myeong-Hwa Song, Ji-Min Noh, Kyung-Seob Kim, Ji-Hyun Choi, Jong-Ho Kim, Chi-Yeon Park, Hyung Joon Joo, Soon Jun Hong, Tae Hee Ko, Jong-Il Choi, Hyo Jin Kim, Jong-Hoon Kim, Se-Hwan Paek, Ji-Na Park, Dong-Hyung Kim, Yongjun Jang, Yongdoo Park, Do-Sun Lim
Summary: The study demonstrates that FGF4 and ascorbic acid can induce differentiation of human embryonic stem cell-cardiogenic mesoderm cells into mature cardiomyocytes, providing a model for adult-onset cardiac disease and drug discovery. These mature cardiomyocytes release acute myocardial infarction biomarkers in response to hypoxic injury, with potential for diagnosis and prognosis in coronary artery diseases based on transcriptome analyses.
Article
Materials Science, Biomaterials
Shuang Wu, Zhao Chen, Xi Yu, Xin Duan, Jialei Chen, Guoming Liu, Min Gong, Fei Xing, Jiachen Sun, Shishu Huang, Zhou Xiang
Summary: Cell-based tissue engineering offers a promising approach for the treatment of bone defects. Urine-derived stem cells (USCs) have been identified as a potential seed cell for bone regeneration. This study successfully developed an injectable BMP2-CSM/Col I hydrogel and demonstrated its ability to promote osteogenic differentiation of USCs in vitro and enhance bone regeneration in vivo.
REGENERATIVE BIOMATERIALS
(2022)
Article
Biochemistry & Molecular Biology
Giovannamaria Petrocelli, Provvidenza Maria Abruzzo, Luca Pampanella, Riccardo Tassinari, Serena Marini, Elena Zamagni, Carlo Ventura, Federica Facchin, Silvia Canaider
Summary: This study investigated the effects of oxytocin on the proliferation, migratory ability, senescence, and autophagy of human adipose-derived stem cells (hASCs). The results showed that oxytocin did not affect hASC proliferation and migratory ability, but promoted autophagy and osteogenic differentiation. This suggests that oxytocin may promote osteogenesis in hASCs by modulating the autophagic process.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Food Science & Technology
Harish K. Handral, Shi Hua Tay, Weng Wan Chan, Deepak Choudhury
Summary: 3D printing technology has significant benefits for the scalable and reproducible production of cultured meat products, addressing issues such as protein, fat, and nutritional content regulation, as well as realistic texture.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2022)
Article
Cell & Tissue Engineering
Marcus Lee, Sathya Kannan, Giridharan Muniraj, Vinicius Rosa, Wen Feng Lu, Jerry Y. H. Fuh, Gopu Sriram, Tong Cao
Summary: The role of angiogenesis in health and disease is gaining attention. Two-photon excitation fluorescence microscopy provides high-quality imaging for studying the molecular mechanisms of angiogenesis. Understanding angiogenesis is important for studying disease responses, tissue model development, and therapeutic strategies.
TISSUE ENGINEERING PART B-REVIEWS
(2022)
Article
Dentistry, Oral Surgery & Medicine
Mi-Ah Kim, Vinicius Rosa, Kyung-San Min
Summary: This study investigated the antibacterial efficacy and cytotoxicity of two graphene derivatives - graphene oxide (GO) and reduced graphene oxide (RGO) - against Enterococcus faecalis in biofilms formed on hydroxyapatite discs. GO and RGO had similar antibacterial effects on E. faecalis biofilms. SEM images showed the ability of GO and RGO to disrupt E. faecalis, while GO had significantly higher cytotoxicity than RGO. The results suggest that RGO has the potential to be used as a more effective disinfecting agent than GO.
DENTAL MATERIALS JOURNAL
(2023)
Article
Engineering, Biomedical
Hardik Makkar, Ying Zhou, Kai Soo Tan, Chwee Teck Lim, Gopu Sriram
Summary: Gingival crevice and gingival crevicular fluid (GCF) flow are crucial for maintaining gingival health and preventing periodontal disease. However, current in vitro models lack the dynamic nature of the gingival crevicular microenvironment, hindering the development of periodontal therapeutics. This study describes a microfluidic platform that mimics GCF flow to culture gingival connective tissue. The platform successfully replicated microbial colonization, biofilm formation, and early gingival inflammation in response to oral symbiont and periodontal pathogen exposure. The induction of flow attenuated the secretion of inflammatory mediators, demonstrating the protective effect of GCF flow.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Pharmacology & Pharmacy
Yub Raj Neupane, Harish K. Handral, Syed Abdullah Alkaff, Wei Heng Chng, Gopalakrishnan Venkatesan, Chenyuan Huang, Choon Keong Lee, Jiong-Wei Wang, Gopu Sriram, Rhonnie Austria Dienzo, Wen Feng Lu, Yusuf Ali, Bertrand Czarny, Giorgia Pastorin
Summary: This study found that biomimetic cell-derived nanovesicles (CDNs) can effectively promote wound healing and tissue regeneration by mimicking extracellular vesicles (EVs). The results showed that CDNs can enhance cell proliferation, cell migration, secretion of growth factors and extracellular matrix proteins, as well as angiogenesis, accelerating wound healing.
ACTA PHARMACEUTICA SINICA B
(2023)
Review
Polymer Science
Harish K. Handral, Tom Adam Wyrobnik, Alan Tin-Lun Lam
Summary: Microcarriers (MCs) are versatile therapeutic tools used in regenerative medicine and drug delivery. They can expand therapeutic cells, serve as tissue engineering scaffolds, and carry drugs and therapeutic compounds. Biodegradable MCs offer solutions to production challenges and can deliver cells at target sites without compromising quality or yield.
Article
Biochemical Research Methods
Toan V. Phan, Yamin Oo, Khurshid Ahmed, Teerapat Rodboon, Vinicius Rosa, Supansa Yodmuang, Joao N. Ferreira
Summary: Hyposalivation and severe dry mouth syndrome are common complications in HNC patients after radiation therapy. Conventional treatment with sialogogues is limited due to the destruction of the salivary gland's secretory parenchyma and reduced regenerative potential. Adipose mesenchymal stem cells (AdMSC) show promise in addressing this issue.
Article
Dentistry, Oral Surgery & Medicine
Arief Cahyanto, Marcos Vinicius Surmani Martins, Otavio Bianchi, Deepa Perumbodathu Sudhakaran, Nikolaos Sililkas, Sergio Graniero Echeverrigaray, Vinicius Rosa
Summary: This study investigated the effects of graphene oxide (GO) on the reliability and lifetime of PMMA. The results showed that GO improved the fatigue resistance and predicted lifetime of PMMA, but had no significant effect on its Weibull parameters.
Article
Dentistry, Oral Surgery & Medicine
A. Cahyanto, P. Rath, T. X. Teo, S. S. Tong, R. Malhotra, B. N. Cavalcanti, L. Z. Lim, K. S. Min, D. Ho, W. F. Lu, V. Rosa
Summary: This study used Taguchi's methods and genetic algorithms to optimize the properties of calcium silicate cements, achieving personalized treatment for patients' specific needs. By simultaneously analyzing multiple input factors, the study successfully designed cement formulations with predicted properties, which were experimentally validated. The study also demonstrated the bioactivity of the cement in dental pulp stem cells.
JOURNAL OF DENTAL RESEARCH
(2023)
Article
Engineering, Biomedical
Hardik Makkar, Chwee Teck Lim, Kai Soo Tan, Gopu Sriram
Summary: Gingival connective tissue and its vasculature are important for the immune response against oral bacteria and act as a connection between the oral and systemic environments. However, there's a lack of models that mimic the complex features of vascularized gingival connective tissue and its interaction with oral bacteria, limiting our understanding of periodontal health and disease. In this study, the researchers developed vascularized gingival tissue models to study the interactions between oral bacteria and gingival tissues in different states. They found that different oral bacteria had different effects on the tissue, with some leading to disease symptoms. The models also showed potential for studying periodontitis and vascular diseases. Overall, the vascularized gingival tissue models provide an in vitro platform for studying host-microbiome interactions and immune response in periodontal health and disease, and may contribute to the development of new periodontal therapeutics.
Article
Engineering, Biomedical
Giridharan Muniraj, Rachel Hui Shuen Tan, Yichen Dai, Ruige Wu, Massimo Alberti, Gopu Sriram
Summary: Researchers have developed an in vitro microphysiological platform to mimic the complex microenvironment of gingiva. The platform allows for the biofabrication of stable gingival equivalents under long-term and air-liquid interface culture with flow conditions. The platform also models a diseased state with disrupted barrier function and assesses the transmucosal permeation of oral-care formulations. The platform provides a miniaturized and integrated platform for host-material and host-microbiome applications in gingival and oral mucosa research.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Dentistry, Oral Surgery & Medicine
Shiyao Liu, Ahmed El-Angbawi, Ruidong Ji, Vinicius Rosa, Nick Silikas
Summary: The objective of this study was to investigate the effects of adding graphene to orthodontic fixed retainer adhesives on colour and optical transmission. The results showed that adding graphene significantly darkened the adhesive colour and reduced translucency, but had no adverse effect on degree of conversion. The optical transmission of the adhesive decreases with increasing graphene concentration.
EUROPEAN JOURNAL OF ORAL SCIENCES
(2023)
Article
Materials Science, Biomaterials
Apurva Mishra, Ren Kai, Srividya Atkuru, Yichen Dai, Filippo Piccinini, Philip M. Preshaw, Gopu Sriram
Summary: The study developed a spheroid-on-chip microfluidic perfusion platform to culture periodontal ligament stem cell (PDLSC) spheroids and investigated the effects of interstitial fluid flow on their morphogenesis, cellular viability, and osteogenic differentiation. The results showed that fluid flow conditions led to larger PDLSC spheroids with improved viability and enhanced osteodifferentiation. This study lays the foundation for future research on the interactions between PDLSC spheroids, microbial pathogens, and biomaterials, and provides insights for the development of innovative therapies, tissue engineering strategies, and regenerative approaches for periodontitis.
BIOMATERIALS SCIENCE
(2023)
Article
Materials Science, Biomaterials
Apurva Mishra, Ren Kai, Srividya Atkuru, Yichen Dai, Filippo Piccinini, Philip M. Preshaw, Gopu Sriram
Summary: In this study, the researchers integrated the spheroid culture of periodontal ligament stem cells (PDLSCs) with a microfluidic perfusion platform to investigate the influence of interstitial fluid flow on PDLSC spheroids. They found that modulation of fluid flow conditions led to larger PDLSC spheroids with improved cellular viability and enhanced osteogenic differentiation. High-flow conditions also resulted in extensive calcium deposition. These findings highlight the importance of fluid flow in shaping the morphological and functional properties of PDLSC spheroids and offer insights for periodontal research.
BIOMATERIALS SCIENCE
(2023)